Sheep Insulin-like growth factor II(IGF2) ELISA kit

Code CSB-EL011088SH
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P10764
Lead Time 5-7 working days
Protein Biological Process 2 glyconeogenesis and glycometabolism
Abbreviation IGF2
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name insulin-like growth factor 2 (somatomedin A)
Alias C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974, OTTHUMP00000011018|OTTHUMP00000011157|insulin-like growth factor 2|insulin-like growth factor II|insulin-like growth factor type 2|putative insulin-like
Species Ovis aries (Sheep)
Protein Biological Process 3 Carbohydrate metabolism
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3 region and to the upstream INS gene at the 5 region. Alternatively spliced transcript variants, encoding either the same or different isoform, have been found for this gene.
HGNC 5466
RGD 2870
MGI 96434
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 86-99  
1:2 Average % 97  
Range % 91-104  
1:4 Average % 95  
Range % 92-98  
1:8 Average % 98  
Range % 91-105  
Recovery
The recovery of sheep IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-97  
EDTA plasma (n=4) 96 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
1000 2.902 2.954 2.928 2.751  
500 2.031 2.164 2.098 1.921  
250 1.089 1.075 1.082 0.905  
125 0.592 0.553 0.573 0.396  
62.5 0.362 0.359 0.361 0.184  
31.25 0.278 0.298 0.288 0.111  
15.6 0.235 0.213 0.224 0.047  
0 0.176 0.177 0.177    
References
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Function The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF-II is influenced by placental lactogen. Also involved in tissue differentiation. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver.
Subcellular Location Secreted
Protein Families Insulin family
Database Links

KEGG: oas:443325

UniGene: Oar.376

Pathway MAPK signaling pathway
PI3K-Akt signaling pathway
Ras signaling pathway

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