Sheep Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Instructions
Code CSB-E13161Sh
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name prolactin
Alternative Names PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
Abbreviation PRL
Uniprot No. P01240
Species Ovis aries (Sheep)
Sample Types serum, plasma, tissue homogenates
Detection Range 5 μIU/mL-2000 μIU/mL
Sensitivity 5 μIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %88-99
1:2Average %94
Range %84-101
1:4Average %93
Range %85-103
1:8Average %90
Range %87-101
Recovery
The recovery of sheep PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9785-104
EDTA plasma (n=4)9588-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/mlOD1OD2Average
20000.154 0.149 0.152
3750.338 0.351 0.345
62.50.542 0.572 0.557
17.50.983 0.959 0.971
51.515 1.529 1.522
02.164 2.077 2.121
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Prolactin acts primarily on the mammary gland by promoting lactation, mammogenesis, mitogenesis and osmoregulation.
Gene References into Functions
  1. These results indicate that PRL, EGF and glucocorticoids have distinct roles in the establishment of beta-casein secretion pathway. PMID: 27836773
  2. Prolactin induces apoptosis of lactotropes PMID: 24859278
  3. Prolactin and epidermal growth factor stimulate adipophilin synthesis in HC11 mouse mammary epithelial cells via the PI3-kinase/Akt/mTOR pathway. PMID: 22426621
  4. Differences in accessibility of the rat arcuate nucleus to sheep prolactin, together with intrinsic differences in neuroendocrine dopaminergic neurons, facilitate homeostatic feedback regulation of sheep prolactin release in the rat hypothalamus PMID: 21953590
  5. conclude that PRL is an important part of the regulation of the pituitary-gonadal system in moderately seasonal DLS rams; it seems to impair release of LH from gonadotrophs, help maintain functional integrity of Leydig cells, & promote testicular growth PMID: 19755483
  6. increased PRL concentration within the central nervous system (CNS) for a few days before oncoming ovulation has no inhibitory effect on tonic LH secretion PMID: 15127327
  7. findings provide strong evidence that the circannual regulation of prolactin secretion, and its associated biology, derive from a pituitary-based timing mechanism PMID: 17185605
  8. Prolactin expressed in adult and fetal sheep brain could be involved in neurodevelopment and/or modulation of the neuroendocrine stress axis. PMID: 18223310
  9. These results demonstrate that TGF-beta abrogates prolactin-stimulated beta-casein gene expression in mammary epithelial cells through, at least in part, a Smad3-dependent mechanism. PMID: 18335503
  10. circulating prolactin decreased (P<0.001) in response to leptin during long days. PMID: 18451065
  11. DNA polymorphism of 5' flanking region of prolactin gene and its association with litter size in sheep. PMID: 19207932
  12. PRL exerts protective effects on adult neurogenesis in the dentate gyrus under chronic stress conditions in mice. PMID: 19211889

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Subcellular Location Secreted
Protein Families Somatotropin/prolactin family
Database Links

KEGG: oas:443317

UniGene: Oar.390

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