Sheep ghrelin/obestatin prepropeptide (GHRL) ELISA kit

Code CSB-EL009414SH
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
ghrelin/obestatin prepropeptide (GHRL)
Abbreviation
GHRL
Species
Ovis aries (Sheep)
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %93
Range %88-98
1:2Average %97
Range %92-102
1:4Average %100
Range %94-106
1:8Average %95
Range %89-100
Recovery
The recovery of sheep GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10195-105
EDTA plasma (n=4)9489-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40001.901 2.011 1.956 1.840
20001.456 1.580 1.518 1.402
10001.033 1.064 1.049 0.933
5000.722 0.753 0.738 0.622
2500.424 0.432 0.428 0.312
1250.302 0.321 0.312 0.196
62.50.207 0.201 0.204 0.088
00.112 0.120 0.116  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Sheep GHRL ELISA Kit was designed for the quantitative measurement of Sheep GHRL protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .

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