Pig Ghrelin (GHRL) ELISA kit

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Target Background

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Product Details

Description

This Pig GHRL ELISA Kit was designed for the quantitative measurement of Pig GHRL protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

Target Name

Ghrelin

Alternative Names

GHRLAppetite-regulating hormone ELISA Kit; Growth hormone secretagogue ELISA Kit; Growth hormone-releasing peptide ELISA Kit; Motilin-related peptide) [Cleaved into: Ghrelin; Obestatin] ELISA Kit

Abbreviation

GHRL

Uniprot No.

Q9GKY5

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	99
	Range %	94-103
1:4	Average %	106
	Range %	100-114
1:8	Average %	95
	Range %	90-100

Recovery

The recovery of pig GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	88	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.481	2.583	2.532	2.382
2000	1.873	1.904	1.889	1.739
1000	1.293	1.221	1.257	1.107
500	0.734	0.708	0.721	0.571
250	0.427	0.462	0.445	0.295
125	0.322	0.302	0.312	0.162
62.5	0.250	0.259	0.255	0.105
0	0.154	0.146	0.150

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.; Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
Gene References into Functions	These expression data may indicate that factors other than ghrelin and leptin are more involved in the regulation of feed intake in pigs apparently acclimated to heat stress. PMID: 27264891 slc30A8 colocalizes with ghrelin and motilin in the gastrointestinal tract of pigs PMID: 26388512 Studied an alternative vascular approach to the modulation of gastric ghrelin levels.. PMID: 25294792 Obestatin enhances proliferation and differentiation of preadipocytes promoting PPARgamma and C/EBPa expression, and inhibiting preadipocyte apoptosis by decreasing expression of Caspase-3, Caspase-7 and Caspase-9. PMID: 24534601 The expression of ghrelin in the digestive tract of low birth weight and normal weight piglets is reported. PMID: 24237675 Data suggest that a dietary factor (here, high levels of dietary copper) is able to up-regulate expression of ghrelin in the fundic gland of growing pigs; the diets tested in this study also increase feed intake and promote weight gain. PMID: 22911428 Ghrelin immunolocalization in the gastrointestinal tract of pigs at different ages were studied and it was concluded that gastric ghrelin expression is not related merely to age but could also potentially be influenced by food intake. PMID: 22281862 BsrI polymorphism at the ghrelin gene locus is potentially associated with carcass and meat quality traits. PMID: 21669497 Plasma concentrations of ghrelin were greater before feeding and decreased after feeding in pigs fed once per day; there was no consistent pattern to ghrelin secretion in the ad libitum fed group. PMID: 20399063 glucose output by primary porcine hepatocytes in suspension culture, after incubation with acylated ghrelin (AG), unacylated ghrelin (UAG), and hexarelin (HEX). PMID: 15536157 Immunization against ghrelin was associated with increased antibody titers, decreased voluntary food consumption, and decreased body weight gain by pigs. PMID: 16793235 ghrelin mRNA expression is up-regulated 10 days after weaning in the gastric fundus of piglets, coinciding with the increase of H(+)-K(+)-ATPase mRNA expression and activity PMID: 16920167 growth hormone stimulated both ghrelin synthesis and secretion in the ovarian follicles PMID: 17951088 Ghrelin requires activation of the NOS/NO route, and its subsequent GC/cGMP signal transduction pathway, as necessary steps to induce GH secretion from somatotrophs. PMID: 18208548 Expression of ghrelin mRNA is affected by estrus in the hypothalamus, pituitary gland and ovary. PMID: 18295988 circulating total plasma ghrelin concentrations in the pig appear to be influenced by chronic changes in energy balance rather than the feeding pattern per se. PMID: 18434347 provides novel evidence that ghrelin has a modulatory effect in the ovary PMID: 19212011 Show More Hide All
Subcellular Location	Secreted.
Protein Families	Motilin family
Database Links	KEGG: ssc:396728 STRING: 9823.ENSSSCP00000012327 UniGene: Ssc.440

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Check Code：

Code	CSB-E09437p
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.