Pig Ghrelin (GHRL) ELISA kit

Instructions
Code CSB-E09437p
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Ghrelin
Alternative Names GHRLAppetite-regulating hormone ELISA Kit; Growth hormone secretagogue ELISA Kit; Growth hormone-releasing peptide ELISA Kit; Motilin-related peptide) [Cleaved into: Ghrelin; Obestatin] ELISA Kit
Abbreviation GHRL
Uniprot No. Q9GKY5
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %89-97
1:2Average %99
Range %94-103
1:4Average %106
Range %100-114
1:8Average %95
Range %90-100
Recovery
The recovery of pig GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8480-88
EDTA plasma (n=4)8884-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1OD2AverageCorrected
40002.481 2.583 2.532 2.382
20001.873 1.904 1.889 1.739
10001.293 1.221 1.257 1.107
5000.734 0.708 0.721 0.571
2500.427 0.462 0.445 0.295
1250.322 0.302 0.312 0.162
62.50.250 0.259 0.255 0.105
00.154 0.146 0.150
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation (By similarity).
Gene References into Functions
  1. These expression data may indicate that factors other than ghrelin and leptin are more involved in the regulation of feed intake in pigs apparently acclimated to heat stress. PMID: 27264891
  2. slc30A8 colocalizes with ghrelin and motilin in the gastrointestinal tract of pigs PMID: 26388512
  3. Studied an alternative vascular approach to the modulation of gastric ghrelin levels.. PMID: 25294792
  4. Obestatin enhances proliferation and differentiation of preadipocytes promoting PPARgamma and C/EBPa expression, and inhibiting preadipocyte apoptosis by decreasing expression of Caspase-3, Caspase-7 and Caspase-9. PMID: 24534601
  5. The expression of ghrelin in the digestive tract of low birth weight and normal weight piglets is reported. PMID: 24237675
  6. Data suggest that a dietary factor (here, high levels of dietary copper) is able to up-regulate expression of ghrelin in the fundic gland of growing pigs; the diets tested in this study also increase feed intake and promote weight gain. PMID: 22911428
  7. Ghrelin immunolocalization in the gastrointestinal tract of pigs at different ages were studied and it was concluded that gastric ghrelin expression is not related merely to age but could also potentially be influenced by food intake. PMID: 22281862
  8. BsrI polymorphism at the ghrelin gene locus is potentially associated with carcass and meat quality traits. PMID: 21669497
  9. Plasma concentrations of ghrelin were greater before feeding and decreased after feeding in pigs fed once per day; there was no consistent pattern to ghrelin secretion in the ad libitum fed group. PMID: 20399063
  10. glucose output by primary porcine hepatocytes in suspension culture, after incubation with acylated ghrelin (AG), unacylated ghrelin (UAG), and hexarelin (HEX). PMID: 15536157
  11. Immunization against ghrelin was associated with increased antibody titers, decreased voluntary food consumption, and decreased body weight gain by pigs. PMID: 16793235
  12. ghrelin mRNA expression is up-regulated 10 days after weaning in the gastric fundus of piglets, coinciding with the increase of H(+)-K(+)-ATPase mRNA expression and activity PMID: 16920167
  13. growth hormone stimulated both ghrelin synthesis and secretion in the ovarian follicles PMID: 17951088
  14. Ghrelin requires activation of the NOS/NO route, and its subsequent GC/cGMP signal transduction pathway, as necessary steps to induce GH secretion from somatotrophs. PMID: 18208548
  15. Expression of ghrelin mRNA is affected by estrus in the hypothalamus, pituitary gland and ovary. PMID: 18295988
  16. circulating total plasma ghrelin concentrations in the pig appear to be influenced by chronic changes in energy balance rather than the feeding pattern per se. PMID: 18434347
  17. provides novel evidence that ghrelin has a modulatory effect in the ovary PMID: 19212011

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Subcellular Location Secreted
Protein Families Motilin family
Database Links

KEGG: ssc:396728

STRING: 9823.ENSSSCP00000012327

UniGene: Ssc.440

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