Guinea pig Interferon γ ,IFN-γ ELISA Kit

Code CSB-E06763Gu
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IFN-γ
Target Name Interferon γ ,IFN-γ
Species Guinea Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 81-92  
1:2 Average % 97  
Range % 92-103  
1:4 Average % 93  
Range % 88-108  
1:8 Average % 101  
Range % 96-105  
The recovery of pig IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 93-102  
EDTA plasma (n=4) 90 86-95  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1200 2.075 2.051 2.063 1.965  
600 1.465 1.441 1.453 1.355  
300 1.069 1.045 1.057 0.959  
150 0.655 0.622 0.639 0.541  
75 0.400 0.387 0.394 0.296  
37.5 0.320 0.295 0.308 0.210  
18.75 0.205 0.223 0.214 0.116  
0 0.101 0.095 0.098    
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