Target Name
pig matrix metalloproteinase 9,MMP-9
Species
Cavia porcellus (Guinea pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 ng/mL-200 ng/ mL
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<10% |
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Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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1:1 |
Average % |
93 |
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Range % |
88-98 |
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1:2 |
Average % |
101 |
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Range % |
91-104 |
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1:4 |
Average % |
90 |
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Range % |
85-93 |
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1:8 |
Average % |
98 |
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Range % |
91-102 |
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Recovery
The recovery of guinea pig MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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Sample Type |
Average % Recovery |
Range |
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Serum (n=5) |
94 |
87-97 |
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EDTA plasma (n=4) |
87 |
81-92 |
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Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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200 |
2.252 |
2.188 |
2.220 |
2.071 |
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100 |
1.635 |
1.676 |
1.656 |
1.507 |
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50 |
0.947 |
0.931 |
0.939 |
0.790 |
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25 |
0.619 |
0.639 |
0.629 |
0.480 |
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12.5 |
0.341 |
0.338 |
0.340 |
0.191 |
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6.25 |
0.236 |
0.231 |
0.234 |
0.085 |
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3.12 |
0.185 |
0.179 |
0.182 |
0.033 |
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0 |
0.150 |
0.148 |
0.149 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx