Human Noradrenaline,NA ELISA Kit

Instructions
Code CSB-E07021h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Noradrenaline,NA
Alternative Names N/A
Abbreviation NA
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.15 pg/mL-600 pg/mL
Sensitivity 0.15 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human NA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-98  
1:2 Average % 85  
Range % 81-89  
1:4 Average % 92  
Range % 88-96  
1:8 Average % 87  
Range % 83-90  
Recovery
The recovery of human NA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 91-99  
EDTA plasma (n=4) 98 95-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
600 0.270 0.278 0.274    
150 0.358 0.368 0.363    
37.5 0.536 0.520 0.528    
9.4 0.687 0.654 0.671    
2.3 1.068 1.066 1.067    
0.59 1.484 1.454 1.469    
0.15 1.598 1.583 1.591    
0 1.730 1.768 1.749    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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