Human S100 calcium binding protein A8/calgranulin A,S100A8 ELISA Kit

CloseMessage

Product Details

Citations

Related Products

Target Data

Most popular with customers

CloseMessage

Product Details

Target Name

S100 calcium binding protein A8

Alternative Names

60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8, OTTHUMP00000015330|S100 calcium-binding protein A8|S100 calcium-binding protein A8 (calgranulin A)|calgranulin A|cystic fibrosis ant

Abbreviation

S100A8

Uniprot No.

P05109

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.25 ng/mL-80 ng/mL

Sensitivity

0.31 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human S100A8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-95
1:2	Average %	95
	Range %	91-99
1:4	Average %	95
	Range %	88-101
1:8	Average %	98
	Range %	91-102

Recovery

The recovery of human S100A8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-93
EDTA plasma (n=4)	95	93-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
80	2.945	2.962	2.954	2.800
40	2.567	2.504	2.536	2.382
20	1.676	1.799	1.738	1.584
10	0.849	0.887	0.868	0.714
5	0.441	0.411	0.426	0.272
2.5	0.263	0.251	0.257	0.103
1.25	0.201	0.211	0.206	0.052
0	0.155	0.152	0.154

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Still Have Questions?

Leave a Message or Start an on-line Chat

Target Data

Function	S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include
Subcellular Location	Secreted, Cytoplasm, Cytoplasm, cytoskeleton, Cell membrane, Peripheral membrane protein
Protein Families	S-100 family
Tissue Specificity	Calprotectin (S100A8/9) is predominantly expressed in myeloid cells. Except for inflammatory conditions, the expression is restricted to a specific stage of myeloid differentiation since both proteins are expressed in circulating neutrophils and monocytes
Database Links	HGNC: 10498 OMIM: 123885 KEGG: hsa:6279 STRING: 9606.ENSP00000357721 UniGene: Hs.416073

Product >> Product Name：
Product >> Code：
Product >> Size：
Name： *
Email： *
Phone：
We recommend you to leave your phone number because occasionally our emails get flagged by spam filters. We would not disclose any of your private information to any third party.
Question/Comment： *
Your Region： *
Country State：
I'd like to receive email newsletters from Cusabio

Yes

No
How did you know this promotion?

Email

Distributor

Search engine

Advertisement

Others
Check Code：

Code	CSB-E11833h
Size	96T,5×96T,10×96T
See More Details

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.