| Code | CSB-E11833h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human S100A8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 83-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-101 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 91-102 | |||||
| The recovery of human S100A8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-93 | ||||
| EDTA plasma (n=4) | 95 | 93-102 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human S100A8 ELISA Kit is engineered for accurate measurement of human S100A8 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the S100A8 content in the sample. The color intensity is positively correlated with S100A8 content in the sample. The S100A8 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.
S100A8 binds to cell surface receptors that trigger signaling pathways to participate in the inflammatory process and plays a key role in multiple cellular processes, including cell survival, cell cycle progression, differentiation, proliferation, and cell migration. High expression of S100A8 has been found in various inflammation-related diseases like inflammatory bowel disease and solid cancers like breast cancer. S100A8 contributes to tumor growth, metastasis development, angiogenesis, and immune escape in a wide variety of solid cancers.
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