S100A8 Antibody

Code CSB-PA020641HA01HU
Size US$299
Image
  • IHC image of CSB-PA020641HA01HU diluted at 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) S100A8 Polyclonal antibody
Uniprot No. P05109
Target Names S100A8
Alternative Names 60B8Ag antibody; AI323541 antibody; B8Ag antibody; BEE11 antibody; CAGA antibody; Calgranulin-A antibody; Calprotectin L1L subunit antibody; Calprotectin; included antibody; CFAG antibody; CGLA antibody; Chemotactic cytokine CP-10 antibody; CP-10 antibody; Cystic fibrosis antigen antibody; L1Ag antibody; Leukocyte L1 complex light chain antibody; MA387 antibody; MIF antibody; Migration inhibitory factor-related protein 8 antibody; MRP-8 antibody; Myeloid-related protein 8 antibody; Neutrophil cytosolic 7 kDa protein antibody; NIF antibody; p8 antibody; Pro-inflammatory S100 cytokine antibody; Protein S100-A8 antibody; S100 calcium binding protein A8 (calgranulin A) antibody; S100 calcium binding protein A8 antibody; S100 calcium-binding protein A8 antibody; S100A8 antibody; S100A8/S100A9 complex; included antibody; S10A8_HUMAN antibody; Urinary stone protein band A antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Protein S100-A8 protein (1-93AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:200-1:500
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include
Gene References into Functions
  1. Study found that that the co-stimulation with both advanced glycation end-products and Porphyromonas gingivalis LPS increased S100A8 and S100A9 mRNA expression in gingival epithelial cells. These results demonstrated that the upregulation of calprotectin in gingival epithelial cells may primarily function intracellularly in this tissue and may contribute to the pathological process of periodontitis. PMID: 28771806
  2. Our results showed that S100A8/A9, absent or present at very low level in skin biopsies from healthy subjects, is dramatically upregulated in each epidermal layer from psoriatic patients. PMID: 29605872
  3. The results suggest that the calcium-binding proteins S100A8 and S100A9 in myeloid cells have an immune regulatory effect. PMID: 29933628
  4. Median MRP8/14 levels were higher in patients with Takayasu arteritis. PMID: 29196802
  5. S100A8 gene knockdown reduced cell proliferation in the HEC-1A cells compared with control cells, induced cell apoptosis, inhibited the phosphorylation of protein kinase B (Akt), and induced the expression of pro-apoptotic genes. PMID: 29595187
  6. Wide intra- and interindividual differences in calprotectin concentrations in the meconium may reflect intestinal inflammation associated with the fetal adaptation to life outside the uterus. PMID: 28463850
  7. Salivary calprotectin levels were significantly higher in RAS [Recurrent aphthous stomatitis] group (23.72 +/- 4.28 mg/L) compared to the HC [Healthy Controls] (21.59 +/- 4.27 mg/L) (P = 0.013). No significant relationship was found between calprotectin levels and age or gender in both groups (P >0.05). PMID: 29519972
  8. the levels of S100A8 and S100A9 in the sera, and calprotectin levels in the sera and urines of bladder cancer patients, were determined. PMID: 28609200
  9. MRP-8/14 concentration increases in coronary artery blood in association with thrombus formation in acute coronary syndrome. PMID: 28986057
  10. ANGPTL6, S100A8 and S100A9 are overexpressed in skin of psoriasis patients versus zero expression in controls. PMID: 27698489
  11. Elevated breast cancer cell and stromal S1008 protein expression are significant indicators of poorer outcomes in early stage breast cancer patients. Evaluation of S100A8 protein expression may provide additional prognostic information beyond traditional breast cancer prognostic biomarkers. PMID: 28717852
  12. S100A8 was required for laser-induced new collagen synthesis in skin cells. PMID: 29270708
  13. S100A8 methylation has a role in diagnosis and prognosis of hepatocellular carcinoma PMID: 27462864
  14. This study demonstrated that S100A8 was detected in the serums of Alzheimer disease patients PMID: 27911324
  15. Studies indicate S100A8 as the most overexpressed gene, and the cell cycle pathway as the most promising biomarker and therapeutic target for relapsed childhood B-acute lymphoblastic leukemia (B-ALL). PMID: 28183295
  16. Low S100A8 expression is associated with head and neck neoplasms. PMID: 26883112
  17. Report role of fecal calprotectin assay in the diagnosis and management of inflammatory bowel disease. PMID: 28735301
  18. S100A8 and S100A9 aggravate Coxsackievirus B3-induced myocarditis and might serve as therapeutic targets in inflammatory cardiomyopathies. PMID: 29158436
  19. Data suggest that fecal calprotectin may be a potential biomarker to identify patients with ankylosing spondylitis (AS) at risk of developing inflammatory bowel disease (IBD). PMID: 28148281
  20. Elevated S100A8 and S100A9 gene expression in SP-infected HMEECs and in the middle ear mucosa of OM, minor co-localized with neutrophil markers suggests that middle ear epithelial cell secretion of S100A8 and S100A9 may play a role in the pathogenesis of recurrent and chronic OM PMID: 28964306
  21. results of this study support an additional role of calprotectin in assessing inflammatory activity in patients with RA PMID: 28832684
  22. Data show that E3 ubiquitin ligase HRD1 (HRD1) decreased the protein level of S100A8 through ubiquitination. PMID: 28423597
  23. High S100A8 expression is associated with glioblastoma. PMID: 27006175
  24. This study implicates S100A8 and S100A9 as important mediators of tumor cell aggressiveness, and highlights the therapeutic potential of S100A8 and S100A9 for interference of metastasis. PMID: 27086923
  25. Data indicate a statistical correlation between fecal calprotectin (FC) and gastrointestinal graft-versus-host disease (GvHD). PMID: 28293713
  26. Perinatal alarmins S100A8 and S100A9 specifically altered MyD88-dependent proinflammatory gene programs. S100 programming prevented hyperinflammatory responses without impairing pathogen defense. TRIF-adaptor-dependent regulatory genes remained unaffected by perinatal S100 programming and responded strongly to lipopolysaccharide, but were barely expressed. PMID: 28459433
  27. Expression of S100A8, S100A9 and S100A12 is modulated by eicosapentaenoic acid and docosahexaenoic acid during Inflammation in adipose tissue and mononuclear cells. PMID: 28125622
  28. High S100A8 expression is associated with lung metastasis in malignant melanoma. PMID: 27151304
  29. Data show that the standardized nerve growth factor (NGF) concentration was negatively correlated with continuous pain, neuropathic pain and total score, and the standardized S100 proteins S100A8/A9 concentration was positively correlated with present pain intensity and continuous pain. PMID: 27936243
  30. S100A8 and S100A9 proteins in concentrations secreted by CD34(-)/CD31(+) circulating angiogenic cells (CACs) with impaired function reduce endothelial cell capillary-like network formation. These effects appear to be mediated by Toll-like receptor 4 and are absent with S100A8 and S100A9 in concentrations secreted by healthy CD34(-)/CD31(+) CACs. PMID: 27793853
  31. MRP8 elevated serum levels in patients with Behcet's disease indicate MRP8 role in the pathogenetic mechanisms of Behcet's disease. PMID: 25471894
  32. High baseline serum MRP8/14 levels are associated with lower survival in Chinese peritoneal dialysis patients. PMID: 22699162
  33. In acute coronary syndromes, MRP8 and MRP8/14 complex are specific ligands of TLR4, which induce the release of TNFalpha and probably other pro-inflammatory agents from monocytes PMID: 21782178
  34. study revealed a novel role of MRP8/14 in the autophagy-mediated elimination of intracellular BCG by promoting reactive oxygen species generation PMID: 25312864
  35. MRP8 has a crucial role in stimulating IL-6 expression by rheumatoid arthritis fibroblast-like synoviocytes. PMID: 23619188
  36. decreased pretreatment levels of myeloid-related protein-8/14 and C-reactive protein may have a role in poor adherence to treatment with tumor necrosis factor inhibitors in juvenile idiopathic arthritis PMID: 27562034
  37. MRP8/14 can activate cells by binding to TLR4, leading to the release of proinflammatory cytokines. These mediators can lead to increased disease activity in enthesitis-related arthritis. PMID: 24231443
  38. Fibronectin adhesive capacity and integrin expression of monocytes of type 1 and type 2 diabetic patients is related to the subjects' serum levels of MRP-8. PMID: 15277376
  39. Excessive release of cytotoxic MRP8/MRP14 by activated phagocytes might therefore present an important molecular pathomechanism contributing to endothelial damage during vasculitis and other inflammatory diseases PMID: 17095618
  40. MRP8 expression is related to the pathological stage of laryngeal squamous cell carcinoma. PMID: 22256739
  41. MRP8/14 in ankylosing spondylitis is associated with peripheral arthritis and active disease PMID: 27738838
  42. Circulating MRP-8/14 is associated with TX-dependent platelet activation in ACS, even during low-dose aspirin treatment, suggesting a contribution of residual TX to MRP-8/14 shedding, which may further amplify platelet activation. PMID: 25037196
  43. Phosphorylation inhibits tubulin polymerization in microtubules. PMID: 15331440
  44. Data suggest that tissue infiltrating macrophages secreting MRP8/14 may contribute to myositis, by driving the local production of cytokines directly from muscle. PMID: 24286299
  45. calprotectin induces IL-6 and MCP-1 production in Human Gingival Fibroblasts via TLR4 signaling that involves MAPK and NF-kappaB, resulting in the progression of periodontitis. PMID: 27925202
  46. Findings suggest that S100A8/A9 may be involved in the inflammatory response with induction of proinflammatory cytokines and may serve as a clinicopathological marker for disease activity in AOSD. PMID: 27537874
  47. Peripheral blood levels of S100A8 and TRAF6 in SAE patients were elevated and might be related to the severity of SAE and predict the outcome of SAE. PMID: 27411454
  48. Low S100A8/A9 levels may contribute to poor wound healing in colonized chronic wounds with striking difference between diabetic foot ulcers and venous leg ulcers. PMID: 27084691
  49. Suggest that, in individuals with lichen planus, S100A8 may exert distinct immunomodulatory and cytotoxicity functions. PMID: 26632637
  50. Serum calprotectin was significantly higher in rheumatoid arthritis patients than controls, and was correlated with disease activity. It was higher in seropositive than in seronegative patients. PMID: 26767827
  51. S100A8, S100A9 and S100A8/A9 protein levels were higher in chronic rhinosinusitis (CRS) with nasal polyps (NP) patients, showing increased deposition on extracellular matrix (ECM) structures in contrast to CRS without NP and controls. PMID: 26493794
  52. MRP8/14 is a good marker of disease activity in rheumatoid arthritis, and higher levels predict response to methotrexate. PMID: 26834220
  53. Blood expression of matrix metalloproteinases 8 and 9 and of their inducers S100A8 and S100A9 supports diagnosis and prognosis of PDAC-associated diabetes mellitus PMID: 26923392
  54. Findings suggest that S100 proteins S100A12 and S100A8/A9 are secreted independently either via distinct mechanisms of secretion or following the activation of different signal transduction pathways. PMID: 27057553
  55. Data suggest up-regulation of pro-inflammatory molecules TLR9, S100A8, and IL-8 plus lower levels of CD11b+ CD33+ myeloid cells may be involved in impairment of wound healing in type 2 diabetes leading to chronic ulcers, such as diabetic foot ulcers. PMID: 26525587
  56. Elevated expression of MRP8/14 are associated with poor prognosis in lupus nephritis. PMID: 26223295
  57. BBP exposure also increased tumor infiltrating myeloid-derived suppressor cell (MDSC) secretion of S100A8/A9, thereby exacerbating the resistance of breast cancer to doxorubicin with cyclophosphamide PMID: 26397389
  58. The frequency of S100A8+ cells in del(5q) myelodysplastic syndrome human bone marrow positively correlates with disease severity. PMID: 26878232
  59. Treg cells suppress the activity of cytotoxic T cells, which kill malaria parasites; therefore, the up-regulation of S100A8 in malaria patients may contribute to pathogen immune escape or tolerance. PMID: 26438270
  60. Oxidative stress associated with inflammation in cystic fibrosis will stop metal sequestration by calprotectin. PMID: 26006104
  61. results demonstrate the functional and mechanistic interplays between CD69 and S100A8/S100A9 in supporting Treg-cell differentiation PMID: 26296369
  62. Serum S100A8 and S100A9 Enhance Innate Immune Responses in the Pathogenesis of Baker's Asthma PMID: 26745257
  63. High S100A8 expression is associated with Refractory Lupus Nephritis. PMID: 26110394
  64. Alarmins S100A8/S100A9 aggravate osteophyte formation in experimental osteoarthritis and predict osteophyte progression in early human symptomatic osteoarthritis. PMID: 25180294
  65. We found that tumor cell proliferation was diminished in the absence of S100A8/A9, leading to significant reduction of tumor size. PMID: 26404752
  66. Low S100A8 mRNA levels were associated with Prostate Cancer. PMID: 25348783
  67. We hypothesize that RAGE and S100A8/A9 contribute to the development of human SCC by modulating keratinocyte growth and migration. PMID: 25811984
  68. This is the first structure-based docking study for the selected protein targets and anticancer drug, and S100A8 and EGFR are attractive anticancer targets and midostaurin with effective drug properties for therapeutic intervention in kidney cancer PMID: 25789858
  69. Calprotectin levels correlated positively, though weakly, with platelet aggregation and activation. PMID: 25970343
  70. Increased soluble gp130 in older patients may inhibit IL-6 trans-signaling, impair barrier function, and decrease S100A8/ S100A9 production in elderly chronic rhinosinusitis patients with asthma and nasal polyps. PMID: 26266960
  71. Serum and mRNA expression levels of S100A8 and S100A9 were found to be upregulated in patients with renal cell carcinoma compared with the other three groups. PMID: 25673070
  72. Severe and prolonged left lower-abdominal pain seems to be the best symptom characterizing symptomatic uncomplicated colonic divertulosis and correlates with fecal calprotectin expression. PMID: 24583746
  73. IL-10 blockade and recombinant IFN-gamma treatment partially abrogated S100A8/A9 mRNA increases in endotoxin tolerance. PMID: 24956170
  74. The increased expression of S100A8 protein in poor prognosis chronic lymphocytic leukemia patients PMID: 24983324
  75. Association of increased S100A8 serum protein with early pregnancy loss PMID: 25252120
  76. Fecal calprotectin levels were significantly higher in patients with pouchitis than those without pouchitis after restorative proctocolectomy for ulcerative colitis. PMID: 25916224
  77. Fecal calprotectin concentrations were significantly higher in Crohn's disease patients with endoscopic recurrence after ileocolonic resection when compared with those in endoscopic remission. PMID: 25781366
  78. Lower fecal calprotectin was associated with mucosal healing in ulcerative colitis assessed by colonoscopy. PMID: 25823769
  79. S100A9 and S100A8 may be involved in inducing damage-associated molecular pattern (DAMP)-mediated inflammation in atopic dermatitis triggered by IL-17A and house-dust mites. PMID: 25308296
  80. Fecal calprotectin appears to be a reliable biomarker of intestinal inflammation and differentiates inflammatory from functional intestinal disorders. PMID: 25369978
  81. Data suggest that intracellular S100A8/A9 contributes to the cancer cell phenotype by modulating matrix metallopeptidase 2 (MMP-2) expression and activity to regulate cell migration and mobility. PMID: 25236491
  82. S100A8 contributes to drug resistance by promoting autophagy in leukemia cells PMID: 24820971
  83. Fecal calprotectin measurement has sufficient sensitivity and NPV values to monitor for Crohn's disease recurrence after intestinal resection. PMID: 25620670
  84. The study showed a high incidence of strains with increased virulence, possessing cagA, vacAs1 and iceA1 genes in symptomatic children with H. pylori infection. PMID: 25228509
  85. S100A8/9 may be an upstream trigger for inflammation and other disease pathways in pterygium. PMID: 24825356
  86. Fecal calprotectin has good diagnostic accuracy in detecting mucosal inflammatory changes in inflammatory bowel disease patients in clinical remission. PMID: 25267955
  87. Circulating MRP8/MRP14 levels in blunt trauma nonsurvivors were significantly lower than those of survivors. Monocytic THP-1 cells stimulated with MRP8/MRP14 expressed the chemokine IFN-gamma inducible protein 10 (IP-10)/CXCL10. PMID: 25342131
  88. Worse overall survival of OPSCC patients with simultaneous reduction of S100A8 and S100A12 expression. PMID: 25302747
  89. These findings establish a novel role for S100A8 in the promoting and enhancing of anaplastic thyroid carcinoma progression PMID: 25423568
  90. TLR9 ligands induce S100A8 in macrophages via a STAT3-dependent pathway which requires IL-10 and PGE2. PMID: 25098409
  91. by independent functional experiments. Our data confirm the primary importance of the TLR4/MRP8 axis in the activation of human monocytes PMID: 25505274
  92. Results do not support an important role for serum S100A8/A9 levels as a biomarker for clinical and structural characteristics in established knee, hip, and hand osteoarthritis patients. PMID: 25179566
  93. these data reveal the capacity of these three S100 proteins to induce MUC5AC production in airway epithelial cells, suggesting that they all serve as key mediators linking neutrophil-dominant airway inflammation to mucin hyperproduction. PMID: 24975020
  94. Data indicate that the plasma levels of S100A9, S100A8, and S100A12 were elevated in acute pancreatitis but a definitive difference between the severe and mild forms of the disease. PMID: 24622086
  95. Glomerular MRP8 expression appears to be associated with progression of proteinuria in obese or type 2 diabetic patients PMID: 24558454
  96. Serum levels of S100A8/S100A9 reflect the degree of inflammation and disease activity in patients with psoriatic arthritis. PMID: 24955375
  97. Calcium dependency on formation of S100A8 or A9 dimers might be the prerequisite for altering monolayer permeability of human umbilical endothelial cells. PMID: 24595267
  98. The aim was to study fecal MMP-9 and HBD-2 in pediatric inflammatory bowel disease to compare their performance to calprotectin and to study whether they would provide additional value in categorizing patients according to their disease subtype. PMID: 24077667
  99. We focus on S100A8 and S100A9 which are involved in the regulation of neutrophil chemotaxis and inflammation related to ocular surface diseases such as dry eye, meibomian gland dysfunction, pterygium, and corneal neovascularization. PMID: 24439044
  100. S100A8/A9 was robustly found to be elevated in the circulation of systemic sclerosis patients. PMID: 24718960

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Subcellular Location Secreted, Cytoplasm, Cytoplasm, cytoskeleton, Cell membrane, Peripheral membrane protein
Protein Families S-100 family
Tissue Specificity Calprotectin (S100A8/9) is predominantly expressed in myeloid cells. Except for inflammatory conditions, the expression is restricted to a specific stage of myeloid differentiation since both proteins are expressed in circulating neutrophils and monocytes
Database Links

HGNC: 10498

OMIM: 123885

KEGG: hsa:6279

STRING: 9606.ENSP00000357721

UniGene: Hs.416073

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