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| Code | CSB-PA020641HA01HU |
| See More Details |  |
| Size | US$299 |
| Uniprot No. | P05109 |
| Image |
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| Protocols | ELISA Protocol Immunohistochemistry (IHC) Protocol |
| Immunogen | Recombinant Human Protein S100-A8 protein (1-93AA) |
| Raised in | Rabbit |
| Species Reactivity | Human |
| Tested Applications | ELISA, IHC; Recommended dilution: IHC:1:200-1:500 |
| Relevance | S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. |
| Form | Liquid |
| Conjugate | Non-conjugated |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method | >95%, Protein G purified |
| Isotype | IgG |
| Clonality | Polyclonal |
| Alias | Protein S100-A8 (Calgranulin-A) (Calprotectin L1L subunit) (Cystic fibrosis antigen) (CFAG) (Leukocyte L1 complex light chain) (Migration inhibitory factor-related protein 8) (MRP-8) (p8) (S100 calcium-binding protein A8) (Urinary stone protein band A), S100A8, CAGA CFAG MRP8 |
| Immunogen Species | Human |
| Research Area | Neuroscience |
| Target Names | S100A8 |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
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| Function | S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include |
| Subcellular Location | Secreted, Cytoplasm, Cytoplasm, cytoskeleton, Cell membrane, Peripheral membrane protein |
| Protein Families | S-100 family |
| Tissue Specificity | Calprotectin (S100A8/9) is predominantly expressed in myeloid cells. Except for inflammatory conditions, the expression is restricted to a specific stage of myeloid differentiation since both proteins are expressed in circulating neutrophils and monocytes |
| Database Links |
HGNC: 10498 OMIM: 123885 KEGG: hsa:6279 STRING: 9606.ENSP00000357721 UniGene: Hs.416073 |
| Pathway | IL-17 signaling pathway |
S100A8 Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-MA0206411A0m | S100A8 Monoclonal Antibody | Human | ELISA,IHC |
S100A8 Antibodies for Human
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA020641HB01HU | S100A8 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA020641HD01HU | S100A8 Antibody, Biotin conjugated | Human | ELISA |
| CSB-PA020641HC01HU | S100A8 Antibody, FITC conjugated | Human | ELISA |
S100A8 Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP020641HU CSB-EP020641HU CSB-BP020641HU CSB-MP020641HU |
Recombinant Human Protein S100-A8(S100A8) | Yeast E.coli Baculovirus Mammalian cell |
S100A8 ELISA Kit for Homo sapiens (Human)
| Code | Product Name | Sample Types | Sensitivity |
|---|---|---|---|
| CSB-E11833h | Human S100 calcium binding protein A8/calgranulin A,S100A8 ELISA Kit | serum, plasma, tissue homogenates | 0.31 ng/mL |
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