Human orosomucoid 2,ORM2 ELISA Kit

Product Details

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Target Background

Product Details

Target Name

orosomucoid 2

Alternative Names

ORM2 ELISA Kit; AGP2Alpha-1-acid glycoprotein 2 ELISA Kit; AGP 2 ELISA Kit; Orosomucoid-2 ELISA Kit; OMD 2 ELISA Kit

Abbreviation

ORM2

Uniprot No.

P19652

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

78 ng/mL-5000 ng/mL

Sensitivity

19.5 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ORM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	98
	Range %	90-102
1:200	Average %	89
	Range %	83-94
1:400	Average %	101
	Range %	93-105
1:800	Average %	105
	Range %	97-110

Recovery

The recovery of human ORM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-95
EDTA plasma (n=4)	94	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
5000	0.131	0.135	0.133
2500	0.389	0.401	0.395
1250	0.750	0.732	0.741
625	1.042	1.020	1.031
312	1.441	1.454	1.448
156	1.650	1.666	1.658
78	1.806	1.767	1.787
0	2.001	2.078	2.040

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human ORM2 ELISA Kit was designed for the quantitative measurement of Human ORM2 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 78 ng/mL-5000 ng/mL and the sensitivity is 19.5 ng/mL.

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Target Background

Function
(From Uniprot)

Functions as transport protein in the blood stream. Binds various hydrophobic ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability. Appears to function in modulating the activity of the immune system during the acute-phase reaction.

Gene References into Functions

the glycosylation change of alpha-1-acid glycoprotein (AGP) in hepatocellular carcinoma (HCC), cirrhosis and controls PMID: 28621608
Neural cell interactions are important for brain physiology and pathology. Particularly, the interaction between non-neuronal cells plays a central role in regulating brain inflammation, which is closely linked to many brain disorders. Here, we newly identified orosomucoid-2 (ORM2) as an endogenous protein that mediates such non-neuronal glial cell interactions PMID: 28193696
Low ORM2 expression is associated with metastasis in hepatocellular carcinoma. PMID: 25965830
Data suggest that human serum albumin (HSA) might serve as a carrier in delivering chitooligomers to target tissues than alpha-1-glycoprotein (AGP) which has pharmacological importance. PMID: 24359035
Alteration in expression of ORM2 suggests that ORM2 could be used as a potential biomarker in the diagnosis of colorectal cancer. PMID: 22363757
the sera of patients with acute inflammation demonstrated increased numbers of bi-antennary and alpha1,3-fucosylated N-glycan structures at each glycosylation site PMID: 15863355
The binding of coumarin enantiomers to ORM2 is studied. PMID: 16290938
Results describe the thermal unfolding and reversibility of temperature-induced changes in alpha(1)-acid glycoprotein. PMID: 16331959
The results indicate that, in accordance with prior expectations, the ORM2 variant is responsible for the acute-phase property of alpha-1 acid glycoprotein. PMID: 19018521
structure of the alpha1-acid glycoprotein (AGP), or orosomucoid (ORM), gene was investigated in a Ghanaian mother and her child, who shared an unusual variant, ORM1 S2(C), found by isoelectric focusing PMID: 11587070

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Subcellular Location

Secreted.

Protein Families

Calycin superfamily, Lipocalin family

Tissue Specificity

Expressed by the liver and secreted in plasma.

Database Links

HGNC: 8499

OMIM: 138610

KEGG: hsa:5005

STRING: 9606.ENSP00000394936

UniGene: Hs.719954

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Code	CSB-E11821h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.