Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
| Code | CSB-E11761h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human PPARGC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 86-99 | |||||
| The recovery of human PPARGC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-100 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
This human PPARGC1A ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human PPARGC1A in different samples, including serum, plasma, cell culture supernates, or cell lysates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of PPARGC1A bound in the initial step.
PPARGC1A is involved in biological functions with implications for insulin action including protection against oxidative stress, formation of muscle fiber types as well as regulation of microvascular flow. It is a chief regulator of energy metabolism and mitochondrial biogenesis by integrating and coordinating the activity of other transcription factors such as Nrf1, nuclear factor 2, PPARα, and mitochondrial transcription A. PPARGC1A is also central to antioxidant defense and redox balance, by regulating the expression of factors such as Nrf2 and MnSOD and promoting NADPH generation, and thereby counters increased ROS levels in highly oxidative cells and protects against inflammation. It is involved in the progression of hormone-associated cancers.
There are currently no reviews for this product.
