Mouse Beta-Endorphin,β-EP ELISA Kit

Code CSB-E06827m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation β-EP
Target Name Beta-Endorphin,β-EP
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 pg/mL-50 pg/mL
Sensitivity 0.195 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 86-92  
1:2 Average % 95  
Range % 91-98  
1:4 Average % 101  
Range % 97-104  
1:8 Average % 92  
Range % 89-95  
Recovery
The recovery of mouse β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 91-98  
EDTA plasma (n=4) 94 90-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
50 2.284 2.298 2.291 2.099  
25 1.782 1.788 1.785 1.593  
12.5 1.141 1.169 1.155 0.963  
6.25 0.638 0.601 0.620 0.428  
3.12 0.459 0.436 0.448 0.256  
1.56 0.369 0.342 0.356 0.164  
0.78 0.298 0.284 0.291 0.099  
0 0.199 0.185 0.192    
References
Still Have Questions? Leave a Message or Start an on-line Chat

Related Products

β-EP ELISA Kit

β-EP ELISA Kit for Homo sapiens (Human)

β-EP ELISA Kit for Rattus norvegicus (Rat)

β-EP ELISA Kit for Bos taurus (Bovine)

β-EP ELISA Kit for Canis lupus familiaris (Dog) (Canis familiaris)

β-EP ELISA Kit for Equus caballus (Horse)

β-EP ELISA Kit for Sus scrofa (Pig)

β-EP ELISA Kit for Oryctolagus cuniculus (Rabbit)

β-EP ELISA Kit for Capra hircus (Goat)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.