Rabbit Collagen Type Ⅱ,Col Ⅱ ELISA Kit

Code CSB-E06856Rb
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rabbit Col ELISA Kit was designed for the quantitative measurement of Rabbit Col protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.

Target Name Collagen Type Ⅱ,Col
Alternative Names Collagen Type 2,Col 2,Collagen Type II,Col II
Abbreviation Col
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 90-102  
1:2 Average % 107  
Range % 97-111  
1:4 Average % 88  
Range % 82-92  
1:8 Average % 98  
Range % 91-102  
Recovery
The recovery of rabbit Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 82-94  
EDTA plasma (n=4) 102 92-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
400 2.576 2.528 2.552 2.457  
200 1.949 1.986 1.968 1.873  
100 1.421 1.366 1.394 1.299  
50 0.827 0.811 0.819 0.724  
25 0.434 0.417 0.426 0.331  
12.5 0.314 0.302 0.308 0.213  
6.25 0.234 0.221 0.228 0.133  
0 0.093 0.097 0.095    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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