Rabbit Leptin,LEP ELISA Kit

Code CSB-E06971Rb
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Leptin,LEP
Abbreviation LEP
Uniprot No. B0KZL0
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 96  
Range % 91-99  
1:200 Average % 93  
Range % 88-97  
1:400 Average % 85  
Range % 80-89  
1:800 Average % 90  
Range % 84-96  
The recovery of rabbit LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 99 94-105  
EDTA plasma (n=4) 87 83-97  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
1000 0.097 0.098 0.098    
500 0.267 0.271 0.269    
250 0.477 0.466 0.472    
125 0.703 0.691 0.697    
62.5 1.104 1.091 1.098    
31.25 1.412 1.429 1.421    
15.6 1.606 1.705 1.656    
0 1.813 1.915 1.864    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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