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Rabbit intercellular adhesion molecule 1,ICAM-1/CD54 ELISA Kit

Instructions
Code CSB-E06974Rb
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name intercellular adhesion molecule 1,ICAM-1/CD54
Abbreviation ICAM-1/CD54
Uniprot No. U3KPG6
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ICAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:5Average %94
Range %91-98
1:10Average %99
Range %91-106
1:20Average %87
Range %82-94
1:40Average %94
Range %86-98
Recovery
The recovery of rabbit ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-96
EDTA plasma (n=4)10096-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
402.694 2.594 2.644 2.501
202.144 2.044 2.094 1.951
101.324 1.284 1.304 1.161
50.754 0.734 0.744 0.601
2.50.536 0.546 0.541 0.398
1.250.351 0.361 0.356 0.213
0.6250.220 0.230 0.225 0.082
00.142 0.143 0.143 　
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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