Recombinant Human Interleukin-15 & Interleukin-15 receptor subunit alpha (IL15 & IL15RA)(N120D) Heterodimer Protein (Active)

The N120D superagonist mutation in this IL-15/IL-15Rα heterodimer enhances trans-presentation signaling, making it a powerful tool for studying JAK/STAT5 pathway activation and NK/T cell biology in immuno-oncology research. Expressed in mammalian cells as a C-terminal Fc-tagged heterodimer (IL-15 aa 31–96 and IL-15Rα aa 49–162), this protein demonstrates an ED50 of 5–20 ng/mL in CTLL-2 cytotoxic T cell proliferation assays, confirming robust bioactivity suitable for cell-based functional studies including primary T cell expansion, PBMC activation, and differentiation assays. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound immune cell readouts, supporting use in sensitive in vivo tumor models and antibody development workflows such as ELISA standard curves or immunogen applications. Purity exceeding 95% by SDS-PAGE, combined with this stringent endotoxin control, provides a suitable basis for reference standard use in cytokine detection assays and NF-κB signaling studies.