ACTB Monoclonal Antibody

Datasheet
Code CSB-MA847175
Product Type Monoclonal Antibody
Size US$258
Immunogen
Raised in Mouse
Species Reactivity Human,Rat,Mouse,Monkey,Dog,Chk,Hamster,Rabbit,Pig,Sheep
Specificity Antibody detects endogenous β-actin protein.
Tested Applications ELISA,WB,IHC,IF;Recommended dilution:WB:1:500-1:5000,IHC:1:50-1:500,IF:1:50-1:200
Relevance β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.
Form Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antigen Affinity Purified
Clonality Monoclonal
Alias ACTB; BRWS1; PS1TP5BP1
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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