EGFP/EYFP Monoclonal Antibody

Datasheet
Code CSB-MA017267
Product Type Monoclonal Antibody
Former Code(s) CSB-MA014303,CSB-MA296408
Size US$384
Immunogen Purified recombinant EYFP.tag full length expressed in E.coli.
Raised in Mouse
Species Reactivity Bacillus cereus
Specificity Transfected
Tested Applications ELISA,WB;Recommended dilution:WB:1:500-1:5000
Relevance

 The green fluorescent protein (GFP) is a protein isolated from the jellyfish Aequorea victoria composed of 238 amino acid residues that exhibits bright green fluorescence. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations. Due to the potential for widespread usage and the evolving needs of researchers, many different mutants of GFP have been engineered. For example, EGFP contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr which result in  brighter green fluorescence. EYFP contains the four amino acid substitutions of Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and Thr-203 to Tyr. EYFP emits yellow fluorescence excited by green light.

Form Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Purification Method Affinity purified
Isotype IgG1
Clonality Monoclonal
Alias GFP; Green Fluorescent Protein; enhanced Green Fluorescent Protein;
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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