Code | CSB-MA221818 |
Size | US$166 |
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Application | Recommended Dilution |
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IHC | 1:20-1:200 |
The monoclonal antibody against human EPCAM was produced using hybridoma technology. The immunogen used for immunizing the mice was a synthesized peptide derived from human EPCAM. B cells were then isolated from the spleen of the immunized mouse and fused with myeloma cells to create hybridomas. The hybridomas were then screened to identify those that produced the EPCAM antibodies and then cultured in the mouse abdominal cavity. The resulting monoclonal antibody was purified from mouse ascites using affinity chromatography with a specific immunogen. This EPCAM monoclonal antibody specifically reacts with human KRT14 protein and has been validated for use in ELISA and IHC applications.
EPCAM is a transmembrane glycoprotein that is expressed in many epithelial tissues. Its main function is to mediate cell adhesion and signaling events between adjacent epithelial cells. EPCAM also plays a role in cell proliferation, differentiation, migration, and apoptosis. In addition, EPCAM is frequently used as a diagnostic marker for epithelial tumors such as breast, colon, and ovarian cancers.
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