Whitlow linker Monoclonal Antibody

Datasheet

Code	CSB-MA234468A0m
Size	US$329
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Image	The Binding Activity of Whitlow linker with Anti-Whitlow linker antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized scFv-based Anti-CD19 CAR recombinant antibody at 2 μg/mL can bind Anti-Whitlow linker antibody. The EC₅₀ is 5.147 to 5.761 ng/mL. Western Blot Positive WB detected in: 293F whole cell lysate, scFv-based Anti-CD19 CAR containing a Whitlow linker (293F/CD19 CAR) whole cell lysate All lanes: Whitlow linker antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 50-200 kDa Observed band size: 50-200 KDa Exposure time：5s Untransfected HEK-293F cells (green line) and transfected scFv-based Anti-CD19 CAR containing a Whitlow/218 linker stable cells (red line) were stained with anti-Whitlow linker antibody (2µg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.
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Product Details
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Product Details

Target Names

Whitlow linker

Alternative Names

Whitlow/218 Linker (E3U7Q); Whitlow linker

Raised in

Mouse

Species Reactivity

Homo sapiens (Human)

Immunogen

Whitlow linker-KLH

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG2b

Clone No.

18A3G5

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	WB：1:1000-1:5000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The production of the Whitlow linker monoclonal antibody includes the multiple injections of the Whitlow linker-KLH into a mouse, collection of the spleen of the immunized mouse and isolation of the B cell-containing splenocytes, fusion of the B cell-containing splenocytes and immortal myeloma cells to form the hybridoma, and culture of the whitlow linker antibody-producing hybridomas. The whitlow linker monoclonal antibody is purified from culture supernatant by Protein G affinity chromatography. Its purity is greater than 95%. In a functional ELISA, immobilized scFv-based anti-CD19 CAR recombinant antibody at 2 μg/mL can bind to this anti-Whitlow linker antibody, with the EC₅₀ 0f 5.147 to 5.761 ng/mL. Apart from ELISA, this antibody has also been successfully validated in WB and FC applications.

The Whitlow linker is widely used in constructing single-chain antibody variable region gene fragments (scFv) and chimeric antigen receptors (CAR). It links the heavy chain (VH) and light chain (VL) regions to ensure the two parts are effectively combined to form a complete antibody structure with antigen-binding ability. Whitlow linker also improves the ability of scFv to resist protease degradation, making it more stable in vivo and in vitro environments. So the whitlow linker monoclonal antibody can be used in preparation, detection, functionality test (ability to activate and kill target cells), and enrichment of CAR.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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