Immobilized Papain

Code CSB-NP005501Pl
Product Type Native Protein
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Relevance Papain is a nonspecific, cysteine-endopeptidase isolated from papaya latex that is used in a wide variety of applications. When IgG molecules are incubated with papain in the presence of cysteine, one or more peptide bonds in the hinge region are split, producing three fragments of similar size: two Fab fragments and one Fc fragment. The Fc fragments can be separated from the Fab fragments using immobilized Protein A or by ion exchange chromatography.
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Tested Applications FuncS
Form Liquid
Storage Buffer PBS, pH 7.4
Alias N/A
Biological_Activity Not test
Purity >95% (SDS-PAGE)
Sequence Full length protein
Research Area Cell Biology
Source Purified from Papaya latex
Protein Names Immobilized Papain
Storage Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
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