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| Code | CSB-MC00491b0105 |
| Product Type | Other Small Molecule Antigen |
| Size | 200 |
| Lead Time | 3-7 business days |
| Carrier Protein | Bovine serum albumin (BSA) |
| Tested Applications | For research use only |
| Storage Buffer | 0.01mol/L, pH 7.4 PBS |
| Purification Method | Dialysis |
| Alias | Anacobin,berubigen,cobalin,cyanocobalamin,dobetin, vibicon |
| Protocol |
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
|
| Storage | Short-term store at 4°C.Long-term store at -20°C. Avoid repeated freeze. |
| Intended Use | ELISA, Immune colloidal gold, Immunochromatography |
| Still Have Questions? | Leave a Message or Start an on-line Chat |
I want to know which groups, the -CONH2 or –OH groups of B12 has been modified and linked to BSA. If it is from CONH2 group, we felt very interested in this kind of conjugate.
Thanks for your reply and information. May I also confirm that the –CONH2 is located in the C ring of B12, like the picture located below?
Several patents and Abbott’s paper mentioned that the “e” –CONH2 modification is best for B12-Sepharose to catch the intrinsic factor or use for IVD purpose. So I assumed you also used this modified B12 conjugate to BSA to initialize the strongest immune response for AB production.
I want to see if you could share any information about the conjugation chemistry used and if there is any spacer arm included for CSB-MC00491b0105 -Vitamin B12-BSA. We want to use NHS-biotin to label this item and want to make sure that the NHS chemistry should work with this protein after your conjugation and if we need to have a long spacer arm to avoid steric hinderance.
