Vitamin B12-BSA conjugate

In Stock
Code CSB-MC00491b0105
Product Type Other Small Molecule Antigen
Size US$200
Lead Time 3-7 business days
Carrier Protein Bovine serum albumin (BSA)
Tested Applications ELISA, Immune colloidal gold, Immunochromatography
Storage Buffer 0.01mol/L, pH 7.4 PBS
Purification Method Dialysis
Alias Anacobin,berubigen,cobalin,cyanocobalamin,dobetin, vibicon
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Storage Short-term store at 4°C.Long-term store at -20°C. Avoid repeated freeze.
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Q&A and Customer Reviews

Q&A
Q:

I want to know which groups, the -CONH2 or –OH groups of B12 has been modified and linked to BSA. If it is from CONH2 group, we felt very interested in this kind of conjugate.

A:
Thanks for your inquiry. The group -OH is unmodified. The group -CONH2 on B12 is activated and then react with BSA directly.
Q:

Thanks for your reply and information. May I also confirm that the –CONH2 is located in the C ring of B12, like the picture located below?
Several patents and Abbott’s paper mentioned that the “e” –CONH2 modification is best for B12-Sepharose to catch the intrinsic factor or use for IVD purpose. So I assumed you also used this modified B12 conjugate to BSA to initialize the strongest immune response for AB production.

A:
Thanks for your response. We can not confirm whether the –CONH2 is located in the C ring of B12. We could only confirm that BSA is conjugated with -CONH2 at one or more positions on Vitamin B12.
Q:

I want to see if you could share any information about the conjugation chemistry used and if there is any spacer arm included for CSB-MC00491b0105 -Vitamin B12-BSA. We want to use NHS-biotin to label this item and want to make sure that the NHS chemistry should work with this protein after your conjugation and if we need to have a long spacer arm to avoid steric hinderance.

A:
Thanks for your email.
CSB-MC00491b0105 Vitamin B12-BSA conjugate
This product does not have any connecting arm. The principle of coupling is that NH2 on Vitamin B12 is combined with BSA.
Vitamin B12-BSA synthesized by this combination will affect the binding to NHS-biotin to some extent.
Any more questions, feel free to contact us. Nice day.
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