Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA. Immobilized CCL5 at 2 μg/ml can bind human GPD1L, the EC50 of human GPD1L protein is 47.34-66.33 μg/ml.

Target Names

GPD1L

Uniprot No.

Q8N335

Research Area

Cell Biology

Alternative Names

GPD1L; KIAA0089; Glycerol-3-phosphate dehydrogenase 1-like protein; GPD1-L; EC 1.1.1.8

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-351aa

Target Protein Sequence

MAAAPLKVCIVGSGNWGSAVAKIIGNNVKKLQKFASTVKMWVFEETVNGRKLTDIINNDHENVKYLPGHKLPENVVAMSNLSEAVQDADLLVFVIPHQFIHRICDEITGRVPKKALGITLIKGIDEGPEGLKLISDIIREKMGIDISVLMGANIANEVAAEKFCETTIGSKVMENGLLFKELLQTPNFRITVVDDADTVELCGALKNIVAVGAGFCDGLRCGDNTKAAVIRLGLMEMIAFARIFCKGQVSTATFLESCGVADLITTCYGGRNRRVAEAFARTGKTIEELEKEMLNGQKLQGPQTSAEVYRILKQKGLLDKFPLFTAVYQICYESRPVQEMLSCLQSHPEHT

Mol. Weight

65.4 kDa

Protein Length

Full Length

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

GPD1L plays a critical role in cardiac sodium channel regulation, with variants linked to Brugada syndrome and sudden cardiac death, making it a compelling target for functional and pharmacological studies. This full-length recombinant human GPD1L (aa 1–351) is expressed in *E. coli* with an N-terminal GST tag positioned to preserve active-site accessibility, and its binding activity has been validated by functional ELISA with an EC50 of 47.34–66.33 μg/ml against immobilized CCL5 at 2 μg/ml. This confirmed functional competence supports use as a positive control in enzyme-linked binding assays, inhibitor screening for IC50 determination, and substrate specificity profiling experiments. Purity exceeding 85% by SDS-PAGE provides a suitable basis for enzymatic activity assays and kinetic parameter analysis where accurate determination of Km and Vmax requires minimal contaminating protein background.

Target Background

Function

Plays a role in regulating cardiac sodium current; decreased enzymatic activity with resulting increased levels of glycerol 3-phosphate activating the DPD1L-dependent SCN5A phosphorylation pathway, may ultimately lead to decreased sodium current; cardiac sodium current may also be reduced due to alterations of NAD(H) balance induced by DPD1L.

Gene References into Functions

The elevated levels of both serum Shh and IL-6 were mainly observed in BC patients who had a significantly higher risk of early recurrence and bone metastasis, and associated with a worse survival for patients with progressive metastatic BC. PMID: 28496128
Then bioinformatic analysis identified potential target sites of the miR-181a located in the 3' untranslated region of GPD1L. Increased GPD1L and decreased miRNA-181a were observed in tissues from osteoarthritis patients. Our results demonstrated that miR-181a may play an important role in the pathogenesis of Osteoarthritis through targeting GPD1L and regulating chondrocyte apoptosis. PMID: 28280258
In a nonreferred nationwide Danish cohort of SIDS cases, up to 5/66 (7.5%) of SIDS cases can be explained by genetic variants in the sodium channel complex genes. PMID: 25757662
The results of real-time PCR showed that, compared with the paired normal tissues, mRNA levels of GPD1L were decreased significantly in head and neck squamous cell carcinoma. PMID: 24274692
Common variations in or near CASQ2, GPD1L, and NOS1AP are associated with increased risk of sudden cardiac death in patients with coronary artery disease PMID: 21685173
hypoxia-induced miR-210 represses GPD1L, contributing to suppression of prolyl hydroxylases activity, and increases of HIF-1alpha protein levels. PMID: 21555452
mutations in GPD1-L as a pathogenic cause for a small subset of sudden infant death syndrome via a secondary loss-of-function mechanism PMID: 17967976
A GPD1-L mutation decreases SCN5A surface membrane expression, reduces inward Na+ current, and causes Brugada syndrome PMID: 17967977
No non-synonymous mutations were found, indicating that GPD1L does not appear to be a major cause of Brugada syndrome in a Japanese population. PMID: 18762705
GPD1L links redox state to cardiac excitability by PKC-dependent phosphorylation of the sodium channel SCN5A. PMID: 19666841
Mutations of GPD1-L may downregulate Na(v)1.5 by altering the oxidized to reduced NAD(H) balance. PMID: 19745168

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Involvement in disease

Brugada syndrome 2 (BRGDA2); Sudden infant death syndrome (SIDS)

Subcellular Location

Cytoplasm. Note=Localized to the region of the plasma membrane.

Protein Families

NAD-dependent glycerol-3-phosphate dehydrogenase family

Tissue Specificity

Most highly expressed in heart tissue, with lower levels in the skeletal muscle, kidney, lung and other organs.

Database Links

HGNC: 28956

OMIM: 272120

KEGG: hsa:23171

STRING: 9606.ENSP00000282541

UniGene: Hs.82432

Code	CSB-EP854029HU
Abbreviation	Recombinant Human GPD1L protein (Active)
MSDS	MSDS Datasheet
Size	US$256
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Glycerol-3-phosphate dehydrogenase 1-like protein (GPD1L) (Active)

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