Recombinant Human Glycerol-3-phosphate dehydrogenase 1-like protein (GPD1L) (Active)

Code CSB-EP854029HU
Size US$256
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Measured by its binding ability in a functional ELISA. Immobilized CCL5 at 2 μg/ml can bind human GPD1L, the EC50 of human GPD1L protein is 47.34-66.33 μg/ml.
Target Names
GPD1L
Uniprot No.
Research Area
Cell Biology
Alternative Names
GPD1L; KIAA0089; Glycerol-3-phosphate dehydrogenase 1-like protein; GPD1-L; EC 1.1.1.8
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-351aa
Target Protein Sequence
MAAAPLKVCIVGSGNWGSAVAKIIGNNVKKLQKFASTVKMWVFEETVNGRKLTDIINNDHENVKYLPGHKLPENVVAMSNLSEAVQDADLLVFVIPHQFIHRICDEITGRVPKKALGITLIKGIDEGPEGLKLISDIIREKMGIDISVLMGANIANEVAAEKFCETTIGSKVMENGLLFKELLQTPNFRITVVDDADTVELCGALKNIVAVGAGFCDGLRCGDNTKAAVIRLGLMEMIAFARIFCKGQVSTATFLESCGVADLITTCYGGRNRRVAEAFARTGKTIEELEKEMLNGQKLQGPQTSAEVYRILKQKGLLDKFPLFTAVYQICYESRPVQEMLSCLQSHPEHT
Mol. Weight
65.4 kDa
Protein Length
Full Length
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The product CSB-EP854029HU is an active recombinant human Glycerol-3-phosphate dehydrogenase 1-like protein (GPD1L). It is produced through several steps: clone the gene encoding full-length human GPD1L (1-351aa) and an N-terminal GST-tag into a plasmid, transfect the recombinant plasmid into the E.coli for protein expression, select the transfected cells by adding the specific antibiotic to the culture medium, harvest the transfected cells and lyse them to release cellular content, including the recombinant human GPD1L, and isolate and purify the recombinant protein. Its purity reaches up to 85%. In a functional ELISA, its activity is measured by binding to the CCL5, with the EC50 of 47.34-66.33 μg/ml.

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Target Background

Function
Plays a role in regulating cardiac sodium current; decreased enzymatic activity with resulting increased levels of glycerol 3-phosphate activating the DPD1L-dependent SCN5A phosphorylation pathway, may ultimately lead to decreased sodium current; cardiac sodium current may also be reduced due to alterations of NAD(H) balance induced by DPD1L.
Gene References into Functions
  1. The elevated levels of both serum Shh and IL-6 were mainly observed in BC patients who had a significantly higher risk of early recurrence and bone metastasis, and associated with a worse survival for patients with progressive metastatic BC. PMID: 28496128
  2. Then bioinformatic analysis identified potential target sites of the miR-181a located in the 3' untranslated region of GPD1L. Increased GPD1L and decreased miRNA-181a were observed in tissues from osteoarthritis patients. Our results demonstrated that miR-181a may play an important role in the pathogenesis of Osteoarthritis through targeting GPD1L and regulating chondrocyte apoptosis. PMID: 28280258
  3. In a nonreferred nationwide Danish cohort of SIDS cases, up to 5/66 (7.5%) of SIDS cases can be explained by genetic variants in the sodium channel complex genes. PMID: 25757662
  4. The results of real-time PCR showed that, compared with the paired normal tissues, mRNA levels of GPD1L were decreased significantly in head and neck squamous cell carcinoma. PMID: 24274692
  5. Common variations in or near CASQ2, GPD1L, and NOS1AP are associated with increased risk of sudden cardiac death in patients with coronary artery disease PMID: 21685173
  6. hypoxia-induced miR-210 represses GPD1L, contributing to suppression of prolyl hydroxylases activity, and increases of HIF-1alpha protein levels. PMID: 21555452
  7. mutations in GPD1-L as a pathogenic cause for a small subset of sudden infant death syndrome via a secondary loss-of-function mechanism PMID: 17967976
  8. A GPD1-L mutation decreases SCN5A surface membrane expression, reduces inward Na+ current, and causes Brugada syndrome PMID: 17967977
  9. No non-synonymous mutations were found, indicating that GPD1L does not appear to be a major cause of Brugada syndrome in a Japanese population. PMID: 18762705
  10. GPD1L links redox state to cardiac excitability by PKC-dependent phosphorylation of the sodium channel SCN5A. PMID: 19666841
  11. Mutations of GPD1-L may downregulate Na(v)1.5 by altering the oxidized to reduced NAD(H) balance. PMID: 19745168

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Involvement in disease
Brugada syndrome 2 (BRGDA2); Sudden infant death syndrome (SIDS)
Subcellular Location
Cytoplasm. Note=Localized to the region of the plasma membrane.
Protein Families
NAD-dependent glycerol-3-phosphate dehydrogenase family
Tissue Specificity
Most highly expressed in heart tissue, with lower levels in the skeletal muscle, kidney, lung and other organs.
Database Links

HGNC: 28956

OMIM: 272120

KEGG: hsa:23171

STRING: 9606.ENSP00000282541

UniGene: Hs.82432

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