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Recombinant Human Beta-defensin 104 protein (DEFB104A) (Active)

Recombinant Human Beta-defensin 104 protein (DEFB104A) (Active)

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Code	CSB-AP003061HU
Abbreviation	Recombinant Human DEFB104A protein (Active)
MSDS	MSDS Datasheet
Size	$142
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Product Details
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Target Background

Product Details

Purity

≥98% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0 ng/ml.

Target Names

DEFB104A

Uniprot No.

Q8WTQ1

Research Area

Microbiology

Alternative Names

BD 4; BD-4; BD4; beta 104; beta 4 Defensin; Beta defensin 4; Beta-defensin 104; Beta-defensin 4; D104A_HUMAN; DEFB-4; DEFB104B; Defensin; Defensin beta 104; Defensin beta 4 precursor; hBD 4; hBD-4; hBD4

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

23-72aa

Complete Sequence

EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP

Mol. Weight

6.0 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 130 mM NaCl

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Human beta-defensin 4 (DEFB104A) plays a critical role in innate mucosal immunity, exhibiting broad-spectrum antimicrobial activity against both bacterial and fungal pathogens. This tag-free recombinant protein, spanning residues 23–72 of the mature sequence, demonstrates confirmed biological activity in a monocyte chemotaxis bioassay at concentrations of 0.1–100.0 ng/ml, supporting its use in innate immunity and host defense research as well as membrane disruption mechanistic studies. The endotoxin level of less than 1.0 EU/μg—verified by LAL method—provides the stringent LPS control essential for MIC determination and bactericidal/fungicidal activity assays, where even trace endotoxin contamination can confound antimicrobial readouts. Purity exceeding 98% by SDS-PAGE, combined with this low endotoxin profile, satisfies the quality thresholds commonly required for drug resistance studies and quantitative dose-response experiments in microbiology.

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Target Background

Function

Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103.

Gene References into Functions

Beta-defensin 104 displays antimicrobial activity against S. carnosus, E. coli, S. cerevisiae, and P. aeruginosa (strong). PMID: 11481241
TFF3 activated the epithelial cells in culture to produce beta defensins 2 (hBD2) and beta defensins 4 PMID: 23198942
Data suggest an activation of b-defensin-4 induction, in human knee meniscus by the osteoarthritis inflammatory process as a result of an endogenous antibiotic defense mechanism accompanied by an intrinsic effort of tissue remodeling. PMID: 21879330
Our results suggest that copy number variation of DEFB4 may not contribute to the pathogenesis of Behcet's disease. PMID: 21385545
Analysis of the DEFB4 promoter region shows remarkably high density of sequence variabilities. PMID: 20445567
The variations in the genes encoding human beta-defensin-1 and -2 may be associated with the risk of severe acute pancreatitis. PMID: 20720450
Data show that beta-defensin cluster (DEFB4, DEFB103 and DEFB104) varied between 2 and 9 copies per genome, and high copy numbers (>4) were underrepresented among patients, suggesting that increased copy numbers could protect from CD. PMID: 20483368
We report here the application of small ubiquitin-related modifier (SUMO) fusion technology to the expression and purification of cationic antibacterial peptide hBD4. PMID: 20526896
This study demonstrates, the involvement of NOD1 and DEFB4 in direct killing of Helicobacter pylori bacteria by epithelial cells and confirms the importance of NOD1 in host defence mechanisms against cagPAI(+)Helicobacter pylori infection. PMID: 20039881
The mRNA level of the beta defensin 4 did not differ significantly between the two subgroups. PMID: 20128731
elevated DEFB4 copy number is a risk factor for Crohn's disease PMID: 19809410
There is an association of four common DEFB104 haplotypes with the risk of prostate cancer in two independent patient cohorts. PMID: 18515986

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Subcellular Location

Secreted.

Protein Families

Beta-defensin family

Tissue Specificity

High expression in the testis. Gastric antrum exhibited relatively high levels. A lower expression is observed in uterus and neutrophils thyroid gland, lung, and kidney. No detectable expression in other tissues tested.

Database Links

HGNC: 18115

KEGG: hsa:107984148

STRING: 9606.ENSP00000322191

UniGene: Hs.559507

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