Recombinant Human Beta-defensin 104 protein(DEFB104A) (Active)

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Code CSB-AP003061HU
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Product Details

Purity >98% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0 ng/ml.
Target Names DEFB104A
Uniprot No. Q8WTQ1
Research Area Microbiology
Alternative Names BD 4; BD-4; BD4; beta 104; beta 4 Defensin; Beta defensin 4; Beta-defensin 104; Beta-defensin 4; D104A_HUMAN; DEFB-4; DEFB104B; Defensin; Defensin beta 104; Defensin beta 4 precursor; hBD 4; hBD-4; hBD4
Species Homo sapiens (Human)
Source E.coli
Expression Region 23-72aa
Complete Sequence EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP
Mol. Weight 6.0 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 130 mM NaCl
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 5-10 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
(From Uniprot)
Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103.
Gene References into Functions
  1. Beta-defensin 104 displays antimicrobial activity against S. carnosus, E. coli, S. cerevisiae, and P. aeruginosa (strong). PMID: 11481241
  2. TFF3 activated the epithelial cells in culture to produce beta defensins 2 (hBD2) and beta defensins 4 PMID: 23198942
  3. Data suggest an activation of b-defensin-4 induction, in human knee meniscus by the osteoarthritis inflammatory process as a result of an endogenous antibiotic defense mechanism accompanied by an intrinsic effort of tissue remodeling. PMID: 21879330
  4. Our results suggest that copy number variation of DEFB4 may not contribute to the pathogenesis of Behcet's disease. PMID: 21385545
  5. Analysis of the DEFB4 promoter region shows remarkably high density of sequence variabilities. PMID: 20445567
  6. The variations in the genes encoding human beta-defensin-1 and -2 may be associated with the risk of severe acute pancreatitis. PMID: 20720450
  7. Data show that beta-defensin cluster (DEFB4, DEFB103 and DEFB104) varied between 2 and 9 copies per genome, and high copy numbers (>4) were underrepresented among patients, suggesting that increased copy numbers could protect from CD. PMID: 20483368
  8. We report here the application of small ubiquitin-related modifier (SUMO) fusion technology to the expression and purification of cationic antibacterial peptide hBD4. PMID: 20526896
  9. This study demonstrates, the involvement of NOD1 and DEFB4 in direct killing of Helicobacter pylori bacteria by epithelial cells and confirms the importance of NOD1 in host defence mechanisms against cagPAI(+)Helicobacter pylori infection. PMID: 20039881
  10. The mRNA level of the beta defensin 4 did not differ significantly between the two subgroups. PMID: 20128731
  11. elevated DEFB4 copy number is a risk factor for Crohn's disease PMID: 19809410
  12. There is an association of four common DEFB104 haplotypes with the risk of prostate cancer in two independent patient cohorts. PMID: 18515986

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Subcellular Location Secreted
Protein Families Beta-defensin family
Tissue Specificity High expression in the testis. Gastric antrum exhibited relatively high levels. A lower expression is observed in uterus and neutrophils thyroid gland, lung, and kidney. No detectable expression in other tissues tested.
Database Links

HGNC: 18115

KEGG: hsa:107984148

STRING: 9606.ENSP00000322191

UniGene: Hs.559507

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