Recombinant Human C-C chemokine receptor type 2(CCR2) (Active)

Code CSB-CF004841HU
Size US$3503Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Activity Measured by its binding ability in a functional ELISA. Immobilized CCR2 at 1 μg/ml can bind human CCL2, the EC50 of human CCL2 protein is 41.51-83.15 μg/ml.
Target Names CCR2
Uniprot No. P41597
Research Area Cancer
Alternative Names C C chemokine receptor type 2; C C CKR 2; C-C chemokine receptor type 2; C-C CKR-2; CC chemokine receptor type 2; CC CKR 2; CC-CKR-2; CCCKR2; CCR 2; CCR-2; CCR1L; CCR2; CCR2_HUMAN; CCR2A; CCR2B; CCR5L; CD192; CD192 antigen ; Chemokine C C motif receptor 2; Chemokine CC Motif Receptor 2; CKR 2; CKR2; CKR2A; CKR2B; CMKBR2; MCP 1 R; MCP-1-R; MCP1 RECEPTOR; MCP1R; Monocyte chemoattractant protein 1 receptor; Monocyte Chemotactic Protein 1 Receptor ; Monocyte Chemotactic Protein 1 Receptor
Species Homo sapiens (Human)
Source in vitro E.coli expression system
Expression Region 1-374aa
Target Protein Sequence MLSTSRSRFIRNTNESGEEVTTFFDYDYGAPCHKFDVKQIGAQLLPPLYSLVFIFGFVGNMLVVLILINCKKLKCLTDIYLLNLAISDLLFLITLPLWAHSAANEWVFGNAMCKLFTGLYHIGYFGGIFFIILLTIDRYLAIVHAVFALKARTVTFGVVTSVITWLVAVFASVPGIIFTKCQKEDSVYVCGPYFPRGWNNFHTIMRNILGLVLPLLIMVICYSGILKTLLRCRNEKKRHRAVRVIFTIMIVYFLFWTPYNIVILLNTFQEFFGLSNCESTSQLDQATQVTETLGMTHCCINPIIYAFVGEKFRSLFHIALGCRIAPLQKPVCGGPGVRPGKNVKVTTQGLLDGRGKGKSIGRAPEASLQDKEGA
Mol. Weight 45.4 kDa
Protein Length Full Length
Tag Info N-terminal 10xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Receptor for the CCL2, CCL7 and CCL13 chemokines
Gene References into Functions
  1. miR-135a-3p serves as a tumour suppressor gene in ovarian cancer by regulating CCR2. PMID: 30138893
  2. this study shows that CCR2 is upregulated on peripheral T cells in osteoarthritis PMID: 30403025
  3. The A allele of CCR2 rs1799864 was associated with a higher MCP-1 level in AD and MCI patients. PMID: 29352259
  4. Study results revealed that CCR2 promotes epithelialtomesenchymal transition through MMP2 in liver cancer. PMID: 30132565
  5. NOX4 is induced in early alcoholic liver injury and regulates CCR2/CCL2 mRNA stability thereby promoting recruitment of inflammatory cells and production of proinflammatory cytokines. PMID: 28383062
  6. T cell-stimulated CLL cells actively recruited monocytes; recruitment critically depended on the C-C-motif-chemokine-receptor-2 axis. PMID: 28971904
  7. Study found that the activation of CCR2 by its ligand CCL2 increased the expression of SMO and Gli-1, resulting in Hh pathway activation, epithelial-mesenchymal transition and hepatocellular carcinoma cell invasion. PMID: 29115520
  8. results confirmed that the CCR2 3'UTR acts as a metastasis suppressor by acting as a ceRNA for STARD13 and thus inhibiting RhoA-ROCK1-MLC-F-actin pathway in breast cancer cells. PMID: 28818997
  9. It can be concluded that MCP-1 and CCR2 polymorphisms are not associated with AgP in Turkish population. PMID: 28458180
  10. beta-arrestin2/AP-1-dependent beta2AR signaling has a critical role in the regulation of CCR2 expression and recruitment of leukocytes to the heart following injury PMID: 27956622
  11. Results indicated that MCP-1 and CCR2 polymorphisms may influence the progression of IgAN, but not increase/decrease its susceptibility PMID: 27788494
  12. High CCR2 expression is associated gastric cancer. PMID: 26992207
  13. Our data strongly support the use of CCR2 and CD180 mRNAs as whole blood pharmacodynamic (PD)biomarkers for BRD4 inhibitors, especially in situations where paired tumor biopsies are unavailable. In addition, they can be used as tumor-based PD biomarkers for hematologic tumors. PMID: 28073847
  14. Through experiments with chimeras of MCP-1 and MCP-3, we identified the chemokine amino-terminal region as being the primary determinant of both the binding and signaling selectivity of these two chemokines at CCR2. PMID: 28536301
  15. Comprehensive Computational Analysis of GWAS Loci Identifies CCR2 as a Candidate Gene for Celiac Disease Pathogenesis PMID: 28059456
  16. Studies indicate manipulating CCL2-CCR2 interaction as a potential approach for combating metastatic disease. PMID: 26885690
  17. Interruption of either CCL2-CCR2 signaling or cathepsin B function significantly impaired perineural invasion (PNI). PMID: 28951461
  18. No statistically significant correlation was found between CRC and the 190 G/A CCR2 polymorphism with colorectal cancer prevalence PMID: 29154245
  19. rs1799864 polymorphism significantly associated with occurrence risk of psoriasis vulgaris in Chinese population PMID: 29145242
  20. CCR2 genetic variants were not associated with risk of atherosclerotic coronary heart disease and glucometabolic traits. PMID: 27013693
  21. These data suggest that CCR1/CCR2B could be involved in clearing EBV-infected latency III B cells in immunocompetent individuals via directing the migration of these cells and attracting the chemokines-expressing immune cells. PMID: 28892735
  22. systemic mRNA expression of CCR2 strongly differentiated children who failed to achieve asthma control with triamcinolone administration PMID: 27665382
  23. Basophil migration into skin lesions of Systemic Lupus Erythematosus patients were observed, but not in normal skin tissue. This migration was related to the upregulation of chemokine receptors CCR1 and CCR2 on basophils. PMID: 28954264
  24. The CCR2 (+190 G/A) GG genotype frequencies for patients were significantly higher in the stage III-IV cancer group ( p = 0.036), and A allele carriers were significantly higher in the stage I-II ovarian cancer group. PMID: 28650671
  25. tumor-promoting role for CCL2 acting through CCR2 on the tumor microenvironment PMID: 27820834
  26. The findings of the work indicate a role for Galphaq and/or Galpha14 and in CCR2a/CCR2b-stimulated Rho A GTPase-mediated serum response factor activation. PMID: 26823487
  27. Endogenous TRAIL/TRAIL-R-mediated CCL2 secretion promotes accumulation of tumor-supportive immune cells in the cancer microenvironment. PMID: 28212753
  28. CCL2/CCR2 axis is expressed in the majority of monocytoid Acute myeloid leukemia blasts PMID: 28045930
  29. CCR2B and beta2AR signals to FLNa to stimulate its endocytosis and recycling to the plasma membrane. PMID: 27909248
  30. this study shows that CYTL1 possesses chemotactic activity and demonstrates that its functional receptor is CCR2B PMID: 27084102
  31. PCSK9 regulates monocyte CCR2 expression and chemotaxis. PMID: 28232185
  32. CCR2-targeted therapy with PF-04136309 in combination with FOLFIRINOX is safe and tolerable PMID: 27055731
  33. Binding of J113863 to CCR2 or CCR5 also induced the recruitment of beta-arrestin 2, whereas UCB35625 did not. UCB35625 induced the chemotaxis of L1.2 cells expressing CCR2 or CCR5. In contrast, J113863 induced the migration of L1.2-CCR2 cells but antagonized the chemokine-induced migration of L1.2-CCR5 cells. PMID: 27895119
  34. CCL2 suppressed HDL internalization and cholesterol efflux via CCR2 induction and p42/44 MAPK activation in ECs. CCL2 induction may contribute to impair HDL function and form atherosclerosis in coronary artery disease. PMID: 27458015
  35. High levels of TRAIL-R3 and CCR-2 expression in tumor epithelial cells identified patients with early breast cancer with poor outcomes. PMID: 28420351
  36. X ray crystal structure of CC chemokine receptor 2 with orthosteric and allosteric antagonists PMID: 27926736
  37. Ingestion of an amino acid supplement immediately post-exercise appears to help maintain plasma MCP-1 concentrations and augment CCR2 expression in resistance trained men. PMID: 27384580
  38. HUMSCs expressing ISZ-sTRAIL might be an efficient therapeutic approach against lung cancer and MCP-1/CCR2 axis is essential for the tumor tropism of HUMSCs PMID: 26733169
  39. The authors demonstrate a positive correlation between CCR2 receptor gene polymorphism and a subgroup of breast cancer showing poor prognosis, which deserves further investigation in larger samples for validation. PMID: 25716470
  40. Results of this study indicated that the CCL2-CCR2 axis was involved in the pathogenesis of oral lichen planus and was positively correlated with disease activity. PMID: 25893607
  41. sCCL2 production possibly will be associated with the adiposity and polymorphic phenotypes of CCL2 and CCR2, in Mexican-Mestizos with insulin resistance PMID: 26839895
  42. Increased CCR2 expression is associated with bladder cancer metastasis. PMID: 26556868
  43. These findings suggested that the CCL2/CCR2 chemokine signaling may regulate the epithelial-mesenchymal transition. PMID: 26648448
  44. CCR2 gene polymorphism (64I allele carriage) is a predisposing factor for allergy. PMID: 27534043
  45. data suggest that the CCR2-64I polymorphism is associated with the protective effect to development of cervical lesions as well as in the protection to HPV 16 infection. PMID: 26982176
  46. Ovarian cancer cells express both Hp and its receptor CCR2 but only after exposure to ascitic fluid, inducing moderated cell migration; results suggest that Hp-induced changes in the cell morphology, actin cytoskeleton structure, and migration ability of tumor cells, is possibly "preparing" these cells for the potential induction of the metastatic phenotype. PMID: 26211665
  47. The frequencies of the rare alleles of CCR2, ITGB3, and 3'UTR of c-fms in the Old Believers are lower than in the sample of Novosibirsk Russians, and the rare allele of DBH is more frequent PMID: 27239844
  48. Among HIV-seropositive samples 3.4% were homozygous CCR2-64I mutants, 17.8%) were heterozygous,78.8% were CCR2 wild type. PMID: 26300579
  49. results suggest that senescent dermal fibroblasts recruit stem cells by secreting various factors and that the CCL2/CCR2 axis is one of the mechanisms underlying this phenomenon PMID: 25808810
  50. Association of hydrogen sulfide with alterations of monocyte chemokine receptors, CCR2 and CX3CR1 in patients with coronary artery disease PMID: 26123579
  51. A polymorphism in MCP-1 may be correlated with the development of non-small cell clung cancer (NSCLC) in the Han nationality of Northern China. However, the polymorphism in CCR-2 is not involved in NSCLC. PMID: 25966143
  52. Results show that nerve-released CCL2 supports prostate cancer migration and perineural invasion though CCR2-mediated signaling. PMID: 25312961
  53. Single nucleotide polymorphism is associated with Non-Small Cell Lung Cancer in Tunisian patients PMID: 25797207
  54. Study shows the increase of CCR2 gene expression in monocytes of severe obstructive sleep apnea patients and that monocytic CCR2 gene expression can be induced under intermittent hypoxia which contributes to the chemotaxis and adhesion of monocytes. PMID: 25411969
  55. The CCR-2 gene polymorphisms may not be genetic risk factors for Parkinson disease in the Han Chinese population, and they do not appear to influence cognitive function and depression in PD patients. PMID: 25370917
  56. The study found a significant positive correlation between CCR2 and CX3CR1 expression on CD8+ cells (r = 0.727, p = 0.0001)in patients with age-related macular degeneration. PMID: 25503251
  57. Data suggest that CCR2 +190 G/A polymorphism may increase the susceptibility to MS, but its action seems to be restricted to individuals who do not possess the major risk allele HLA-DRB1*15:01. PMID: 25604634
  58. CCR2 is directly involved in the detrimental infiltration of neutrophils into the joints in patients with rheumatoid arthritis (RA), showing a new inflammatory role of CCR2 during RA flares or active disease. PMID: 25779331
  59. The CCR2 mRNA were significantly increased in demyelinated Multiple Sclerosis hippocampus. PMID: 25149422
  60. There is no association between CCR2 gene rs1799864 polymorphism and the risk for hemophagocytic lymphohistiocytosis(HLH) in children. PMID: 25760842
  61. The results confirm that variants of CCR5 and CCR2 genes and their haplotypes are associated with the severity but not with susceptibility to develop chagasic cardiomyopathy. PMID: 25312802
  62. HCMV infection is able to activate the CCL2-CCR2 interactions to further enhance HCMV infection and/or replication PMID: 25261725
  63. Data indicate that coupling selectivity of chemokine receptors CCR2, CCR5, and CCR7 with G protein subtypes was measured and compared. PMID: 25614627
  64. These findings suggest that CCR2, CX3CR1, RANTES and SDF1 gene variants seem to play an important role in the dynamics of HIV infection and could be used as drug or vaccine targets. PMID: 25313609
  65. A significantly enhanced risk for prostate cancer due to interactions between CCL2 and CCR2 gene variants. PMID: 25266801
  66. These results show that intermediate monocytes are increased in frequency soon after infection; express CCR2, which would promote their migration into the lesions; and, owing to their production of TNF, can enhance the inflammatory response. PMID: 25139016
  67. We noticed higher prevalence of CCR2 A allele as well as lower frequency of CCR5 d32 in chronic urticaria group in comparison to control group PMID: 23727176
  68. results support a model of CCR2 equilibrium: chemokine binding changes the conformational equilibrium of CCR2 toward the active state, and Pro-C switches its binding partner from the membrane to FROUNT PMID: 25283965
  69. Unique properties of human beta-defensin 6 (hBD6) and glycosaminoglycan complex: sandwich-like dimerization and competition with the chemokine receptor 2 (CCR2) binding site. PMID: 24970887
  70. IL-33 enhances proliferation and invasiveness of decidual stromal cells by up-regulation of CCL2/CCR2 via NF-kappaB and ERK1/2 signaling. PMID: 24344240
  71. This brief review summarizes our current understanding of the physiologic and pathologic roles of CCR2, focusing on its involvement in CNS inflammatory diseases. PMID: 24984897
  72. genetic polymorphism is not associated with ischemic stroke in Armenian population PMID: 24083412
  73. Findings suggests that CCR2 expression is crucial for CCL2-induced proliferation and invasion of PC-3M cells. PMID: 24406043
  74. In a population of women from Taiwan, an SNP in CCR-2 was not found to be associated with cervical cancer. PMID: 23427179
  75. CCR5-Delta32 allele is not responsible for HIV-1 resistance in this HESN group; however, the CCR2-I allele could be protective, while the 29G allele might increase the likelihood of acquiring HIV-1 infection PMID: 24098976
  76. cross-desensitization of CCR1 by FPR1 was associated with CCR1 phosphorylation and moderate reduction of CCR1 cell-surface expression. In contrast, CCR2 was not phosphorylated or internalized after FPR1 activation. PMID: 24778447
  77. Data suggest that CCL13 binds to several chemokine receptors (CCR1, CCR2, and CCR3), allowing CCL13 to elicit different effects on target cells of immune system. CCL13/CCR2 signal transduction induces dendritic cell maturation/activation. [REVIEW] PMID: 23846739
  78. No association was found between the CCR2V64Il polymorphism and susceptibility to migraine or headache severity in the Iranian population. PMID: 24453913
  79. CCL2-CCR2 axis regulates macrophage polarization by influencing the expression of functionally relevant and polarization-associated genes and downmodulating proinflammatory cytokine production. PMID: 24639350
  80. indicate that three genes, namely Chemokine (C-X-C motif) receptor 2 (CXCR2), C-C chemokine receptor type 2 (CCR2) and E1A-Binding Protein P400 (EP400), were able to identify HCC individually with accuracies of 82.4%, 78.4% and 65%, respectively. PMID: 24332572
  81. These data demonstrate a linkage disequilibrium between CCR2 and CCR5 at several loci. PMID: 24011637
  82. Data suggest signaling via CCL2 (chemokine [C-C motif] ligand 2) and CCR2 (CCL2 receptor) is involved in pathogenesis of neurodegenerative/neuroinflammatory disorders such as Alzheimer disease, multiple sclerosis, and ischemic brain injury. [REVIEW] PMID: 23771498
  83. The entry of HIV infected and uninfected CD14(+)CD16(+) monocytes into the brain was facilitated by significantly increased surface JAM-A, ALCAM, CD99, and PECAM-1, as compared to CD14(+) cells that are CD16 negative. PMID: 23922698
  84. This meta-analysis suggests that genetic polymorphisms of CCR2 V64I may influence the susceptibility of cancer in Asian countries.[meta-analysis] PMID: 23876399
  85. CCL2 produced by senescent biliary epithelial cells may promote infiltration of corresponding CCR2-expressing cells and further aggravate inflammation in bile duct lesion in primary biliary cirrhosis. PMID: 24185682
  86. Our data demonstrate that the monocyte-specific chemokine CCL7 and its receptor CCR2 are expressed in tumour cells of RCC PMID: 24327013
  87. A functional binding element in helix 8 of CCR2 is described for the cytosolic regulator FROUNT. PMID: 24128342
  88. Our data suggest the influence of CCR2-64I variant in the development of prostate cancer. PMID: 23632061
  89. CCL2 rs1024611 and CCR2 rs1799864 polymorphisms is not associated with myocardial infarction. PMID: 24078580
  90. MCP-1 -2518A/G and CCR2 190G/A polymorphisms are risk factors for renal cell carcinoma. PMID: 23657965
  91. No significant association has been found between MCP-1, CCR2 and CCR5 polymorphisms and atopic asthma in Tunisian patients. PMID: 23454776
  92. Structure prediction and molecular dynamics simulations of a G-protein coupled receptor: human CCR2 receptor PMID: 22909007
  93. CCR2-64I (A/A genotype) may account for the protection against HIV-1 infection in population from west region of Cameroon. PMID: 23880174
  94. CCR2 is absent from human adipose lineage cells. PMID: 23376609
  95. This work identifies a novel protective role for CCL2/CCR2 in the tumor microenvironment PMID: 23686489
  96. The proportion of CCR2+ monocytes and CCR2 MFI were correlated with body weight, body mass index, fat mass, insulin and HOMA-IR. Oxidative burst also correlated with anthropometric measures, fat mass and expression of CCR2. PMID: 23374597
  97. there were strong gene-gene interactions between the CCR5 promoter and CCR2 polymorphisms on the susceptibility of HIV-1 infection PMID: 23057571
  98. CCR2-V64I may contribute to an increased risk of bladder, cervical and oral cancer.[meta-analysis] PMID: 23612252
  99. that CCR2 expression is associated with lower MMSE scores in an older human population PMID: 22607625
  100. Two hundred and twenty-one samples from HIV-infected subjects in Thailand were genotyped for the CCR5, CCR2, and SDF1 mutation. Results demonstrated that there was a significant association between CD4+ T-cell numbers and CCR2 and SDF1 polymorphisms. PMID: 23325742

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Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Expressed by monocytes and IL2-activated NK cells.
Database Links

HGNC: 1603

OMIM: 601267

KEGG: hsa:729230

STRING: 9606.ENSP00000292301

UniGene: Hs.511794

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