Code | CSB-CF004841HU |
MSDS | |
Size | Pls inquire |
Source | in vitro E.coli expression system |
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The recombinant human C-C chemokine receptor type 2 (CCR2) protein is a cell-free system in vitro E.coli expressed full-length protein with good activity. In cell-free systems, the plasmid vector containing the gene that encodes the full-length CCR2 protein (1-374aa) can be induced to express the protein in vitro using extracts of whole cells that are compatible with translation. The purity of this recombinant human CCR2 protein is greater than 85% as measured by SDS-PAGE. Its activity has been validated in a functional ELISA.
CCR2 is a protein found on certain immune cells like monocytes and macrophages, guiding them to areas of injury and inflammation [1]. It interacts with different proteins like CCL2, CCL7, CCL8, CCL12, and CCL13 [2]. When CCR2 is activated, it triggers signals inside cells that control how they stick to surfaces and move around, especially in macrophages [3]. This protein is crucial for bringing inflammatory cells to sites of inflammation and also plays a part in bone cell development [4][5]. It's involved in various health conditions, including cancer, kidney injuries, cognitive problems from head radiation, and heart failure [6][7][8][9]. Scientists have found that CCR2 might affect inflammation, brain cell growth, brain cell activity, and thinking abilities [6]. In breast cancer, CCR2 seems to help cancer cells grow and spread [10]. It also helps regulate levels of certain signaling molecules in the blood [11]. Some researchers think that targeting the CCL2–CCR2 pathway could be a way to treat traumatic brain injuries [12].
References:
[1] F. Kumase, K. Takeuchi, Y. Morizane, J. Suzuki, H. Matsumoto, K. Kataokaet al., Ampk-activated protein kinase suppresses ccr2 expression by inhibiting the nf-κb pathway in raw264.7 macrophages, Plos One, vol. 11, no. 1, p. e0147279, 2016. https://doi.org/10.1371/journal.pone.0147279
[2] L. Xu, Q. Fang, Y. Miao, M. Xu, Y. Wang, L. Sunet al., The role of ccr2 in prognosis of patients with endometrial cancer and tumor microenvironment remodeling, Bioengineered, vol. 12, no. 1, p. 3467-3484, 2021. https://doi.org/10.1080/21655979.2021.1947631
[3] W. Fang, I. Jokar, A. Zou, D. Lambert, P. Dendukuri, & N. Cheng, Ccl2/ccr2 chemokine signaling coordinates survival and motility of breast cancer cells through smad3 protein- and p42/44 mitogen-activated protein kinase (mapk)-dependent mechanisms, Journal of Biological Chemistry, vol. 287, no. 43, p. 36593-36608, 2012. https://doi.org/10.1074/jbc.m112.365999
[4] R. Cherney, R. Mo, M. Yang, Z. Xiao, Q. Zhao, S. Mandlekaret al., Alkylsulfone-containing trisubstituted cyclohexanes as potent and bioavailable chemokine receptor 2 (ccr2) antagonists, Bioorganic & Medicinal Chemistry Letters, vol. 24, no. 7, p. 1843-1845, 2014. https://doi.org/10.1016/j.bmcl.2014.02.013
[5] Z. Xing, C. Lu, D. Hu, Y. Yu, X. Wang, C. Colnotet al., Multiple roles for ccr2 during fracture healing, Disease Models & Mechanisms, vol. 3, no. 7-8, p. 451-458, 2010. https://doi.org/10.1242/dmm.003186
[6] K. Bélarbi, T. Jopson, C. Arellano, J. Fike, & S. Rosi, Ccr2 deficiency prevents neuronal dysfunction and cognitive impairments induced by cranial irradiation, Cancer Research, vol. 73, no. 3, p. 1201-1210, 2013. https://doi.org/10.1158/0008-5472.can-12-2989
[7] P. Mittal, L. Wang, T. Akimova, C. Leach, J. Clemente, M. Senderet al., The ccr2/mcp-1 chemokine pathway and lung adenocarcinoma, Cancers, vol. 12, no. 12, p. 3723, 2020. https://doi.org/10.3390/cancers12123723
[8] K. Furuichi, T. Wada, Y. Iwata, K. Kitagawa, K. Kobayashi, H. Hashimotoet al., Ccr2 signaling contributes to ischemia-reperfusion injury in kidney, Journal of the American Society of Nephrology, vol. 14, no. 10, p. 2503-2515, 2003. https://doi.org/10.1097/01.asn.0000089563.63641.a8
[9] G. Bajpai, C. Schneider, N. Wong, A. Bredemeyer, M. Hulsmans, M. Nahrendorfet al., The human heart contains distinct macrophage subsets with divergent origins and functions, Nature Medicine, vol. 24, no. 8, p. 1234-1245, 2018. https://doi.org/10.1038/s41591-018-0059-x
[10] G. Brummer, W. Fang, C. Smart, B. Zinda, N. Alissa, C. Berklandet al., Ccr2 signaling in breast carcinoma cells promotes tumor growth and invasion by promoting ccl2 and suppressing cd154 effects on the angiogenic and immune microenvironments, Oncogene, vol. 39, no. 11, p. 2275-2289, 2019. https://doi.org/10.1038/s41388-019-1141-7
[11] B. Zhao, J. Campbell, C. Salanga, L. Ertl, Y. Wang, S. Yauet al., Ccr2-mediated uptake of constitutively produced ccl2: a mechanism for regulating chemokine levels in the blood, The Journal of Immunology, vol. 203, no. 12, p. 3157-3165, 2019. https://doi.org/10.4049/jimmunol.1900961
[12] Y. Jiang, Y. Chen, C. Huang, A. Xia, G. Wang, & S. Liu, Hyperbaric oxygen therapy improves neurological function via the p38-mapk/ccl2 signaling pathway following traumatic brain injury, Neuroreport, vol. 32, no. 15, p. 1255-1262, 2021. https://doi.org/10.1097/wnr.0000000000001719
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