Recombinant Human Carbonic anhydrase 5B, mitochondrial (CA5B) (Active)

Mitochondrial carbonic anhydrase 5B plays a specialized role in bicarbonate supply for ureagenesis and gluconeogenesis, making it a distinctive target for metabolic enzyme research. This recombinant human CA5B, spanning residues 34–317 of the mature protein, demonstrates validated esterase activity of 233 pmol/min/μg, providing a reliable foundation for kinetic parameter analysis (Km, Vmax, kcat), substrate specificity profiling, and inhibitor screening with IC50 determination. The C-terminal 6xHis tag preserves active-site accessibility, which supports accurate specific activity determination and drug candidate evaluation without steric interference at the catalytic zinc center. Purity exceeding 95% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typically required for precise kinetic measurements, positive controls in enzyme-linked assays, and structural studies such as crystallography.