Recombinant Human Inositol 1,4,5-trisphosphate receptor-interacting protein-like 1 (ITPRIPL1), partial (Active)

In Stock
Code CSB-MP756966HU
Abbreviation Recombinant Human ITPRIPL1 protein, partial (Active)
MSDS
Size $138
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Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized Human ITPRIPL1 at 2 μg/ml can bind Anti-ITPRIPL1 recombinant antibody (CSB-RA756966MA1HU). The EC50 is 6.537-7.663 ng/mL. Biological Activity Assay
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Product Details

Purity
≥ 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human ITPRIPL1 at 2 μg/mL can bind Anti-ITPRIPL1 recombinant antibody (CSB-RA756966MA1HU). The EC50 is 6.537-7.663 ng/mL.
Target Names
Uniprot No.
Alternative Names
ITPRIPL1; KIAA1754L; Inositol 1,4,5-trisphosphate receptor-interacting protein-like 1
Species
Homo sapiens (Human)
Source
Mammalian cell
Expression Region
25-103aa
Target Protein Sequence
HPLMVSDRMDLDTLARSRQLEKRMSEEMRLLEMEFEERKRAAEQRQKAENFWTGDTSSDQLVLGKKDMGWPFQADGQEG
Mol. Weight
10.7 kDa
Protein Length
Partial
Tag Info
C-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

ITPRIPL1 participates in inositol trisphosphate receptor signaling pathways, yet functional reagents covering defined interaction domains remain limited for mechanistic studies. This mammalian-expressed fragment spanning residues 25–103 demonstrates quantifiable antibody-binding activity with an EC50 of 6.537–7.663 ng/mL in functional ELISA, providing a validated tool for epitope mapping studies and antibody specificity profiling against this receptor-interacting protein. The C-terminal 10xHis tag enables straightforward immobilization for surface plasmon resonance experiments and protein-protein interaction screens aimed at identifying novel ITPRIPL1 binding partners. With purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this preparation meets the quality thresholds commonly required for immunoassay development and supports use as a positive control in binding assays where reproducible antibody recognition is essential.

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Target Background

Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
ITPRIP family
Database Links

HGNC: 29371

KEGG: hsa:150771

STRING: 9606.ENSP00000355121

UniGene: Hs.65009

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
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