ITPRIPL1 Recombinant Monoclonal Antibody

ITPRIPL1, or inositol 1,4,5-trisphosphate receptor-interacting protein-like 1, represents an emerging target in cell signaling research. As a member of the IP3 receptor-interacting protein family, ITPRIPL1 is implicated in calcium signaling pathways that regulate diverse cellular processes. Understanding its function and expression patterns offers researchers valuable insights into fundamental cell biology and potential therapeutic targets.

This recombinant monoclonal antibody against ITPRIPL1 provides the consistency and reliability that demanding research applications require. Produced using recombinant technology with a defined sequence, this clone 6D11 antibody eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies. The human IgG4 isotype with the S228P stabilizing mutation ensures structural integrity, while affinity chromatography purification delivers high purity suitable for sensitive detection methods.

Functional ELISA validation demonstrates exceptional binding characteristics across multiple species. Against human ITPRIPL1, the antibody achieves an EC50 of 6.5–7.7 ng/mL, while binding to Macaca fascicularis ITPRIPL1 shows comparable sensitivity with an EC50 of 3.8–4.4 ng/mL. Notably, the antibody exhibits particularly strong affinity for mouse Itpripl1, with an EC50 of 0.75–0.99 ng/mL. This cross-species reactivity spanning human, cynomolgus macaque, and mouse systems provides flexibility for researchers working across translational models.

The recommended ELISA dilution range of 1:5,000 to 1:50,000 reflects the antibody's high sensitivity, allowing researchers to optimize conditions for their specific experimental systems. For investigators studying calcium signaling mechanisms, receptor interactions, or exploring ITPRIPL1 as a potential biomarker, this antibody offers a dependable tool for quantitative detection across key research species.