Product Details

Purity

≥ 95% as determined by SDS-PAGE.
≥ 95% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human IL1RAP at 2 μg/mL can bind Anti-IL1RAP recombinant antibody (CSB-RA878844MA1HU). The EC50 is 0.9667-1.477 ng/mL.

Target Names

IL1RAP

Uniprot No.

Q9NPH3

Alternative Names

C3orf13, IL1R3

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

21-359aa

Target Protein Sequence

SERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKFNYSTAHSAGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNSPMKLPVHKLYIEYGIQRITCPNVDGYFPSSVKPTITWYMGCYKIQNFNNVIPEGMNLSFLIALISNNGNYTCVVTYPENGRTFHLTRTLTVKVVGSPKNAVPPVIHSPNDHVVYEKEPGEELLIPCTVYFSFLMDSRNEVWWTIDGKKPDDITIDVTINESISHSRTEDETRTQILSIKKVTSEDLKRSYVCHARSAKGEVAKAAKVKQKVPAPRYTVE

Mol. Weight

40.5 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

IL1RAP functions as an essential co-receptor in the IL-1 signaling complex, where it stabilizes ligand binding and amplifies inflammatory responses—making it a target of interest in autoimmune disease research and therapeutic antibody development. This mammalian-expressed construct spanning residues 21–359 captures the complete extracellular domain responsible for receptor assembly and demonstrates quantifiable binding activity with an EC50 of 0.97–1.48 ng/mL against an anti-IL1RAP recombinant antibody in functional ELISA, confirming native-like conformational integrity. The validated binding performance supports its use in competitive inhibition assays for blocking antibody screening, therapeutic antibody epitope mapping, and ligand-receptor interaction studies via ELISA, surface plasmon resonance, or biolayer interferometry. Purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg align with standards expected in cell-based functional assays and antibody affinity characterization workflows.

Target Background

Function

Coreceptor for IL1RL2 in the IL-36 signaling system. Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Coreceptor for IL1RL1 in the IL-33 signaling system. Can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD. May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (Probable).; Associates with secreted ligand-bound IL1R2 and increases the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Enhances the ability of secreted IL1R1 to inhibit IL-33 signaling.; Unable to mediate canonical IL-1 signaling. Required for Src phosphorylation by IL1B. May be involved in IL1B-potentiated NMDA-induced calcium influx in neurons.

Gene References into Functions

Expression level of sIL1RAP may become one of the potential indexes for determining the prognosis of low-grade gliomas PMID: 29238884
These results provide novel insights into the role of IL1RAP in CML and a strong rationale for the development of an IL1RAP antibody therapy to target residual CML stem cells. PMID: 27621309
single nucleotide polymorphism A471T in the Toll-interleukin 1 receptor domain (TIR) of the IL-1Rrp2 that is present in approximately 2% of the human population, down-regulated IL-36R signaling by a decrease of interaction with IL-1RAcP. PMID: 27307043
The findings of this study support IL1RAP as a novel potential Alzheimer's disease target and highlight the use of amyloid PET as a valuable Alzheimer's disease endophenotype, particularly in a longitudinal framework. PMID: 26268530
The SNP rs4624606 in IL-1RAcP was nominally associated with CAD risk. PMID: 25517029
Data suggest serum levels of soluble IL1RAP (from alternative splicing) are down-regulated in endometriosis throughout menstrual cycle; IL1RAP levels (which peak in proliferative phase in fertile women) exhibit minor variations in endometriosis. PMID: 24935223
Plasma sIL1RAP levels are reduced in obesity and can potentially act as biomarkers of obesity. PMID: 24915116
Combined crystallography and small-angle X-ray-scattering studies reveal that ST2 possesses hinge flexibility between the D3 domain and D1D2 module, whereas IL-1RAcP exhibits a rigid conformation in the unbound state in solution. PMID: 23980170
Knockdown of IL1RAP decreased clonogenicity and increased cell death of AML cells. PMID: 22723552
Interleukin-1R3 mediates interleukin-1-induced potassium current increase through fast activation of Akt kinase. PMID: 22778412
decreased protein levels in the peritoneal fluid of women with endometriosis PMID: 21958553
Studies indicate that new targets have recently been investigated as potential modulators in myeloid leukemia pathogenesis, including miR-328, BMI1, FOXOs and IL1RAP. PMID: 21373837
This study showed a significant downregulation of soluble interleukin-1 receptor accessory protein expression in the endometrium of women with endometriosis. PMID: 21272866
identifies IL1RAP as a unique cell surface biomarker distinguishing Ph(+) from Ph(-) candidate chronic myeloid leukemia (CML) stem cells and opens up a previously unexplored avenue for therapy of CML PMID: 20805474
Identification of essential regions in the cytoplasmic tail of interleukin-1 receptor accessory protein critical for interleukin-1 signaling PMID: 11880380
soluble form of IL-1R AcP contributes to the antagonism of IL-1 action by the type II decoy receptor PMID: 12530978
The dramatic changes in levels of IL-1RAcP mRNAs suggest important functions in regulating sensitivity to IL-1 during stress and may play a role in oncogenic processes that are engaged during chronic inflammation. PMID: 12781872
IL-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP in Jurkat cells PMID: 14734551
Specific destabilization of membrane-bound IL-1 receptor accessory protein mRNA in HepG2 hepatoma cells is mediated by its 2.8-kilobase 3'-untranslated region. PMID: 15528363
Analysis of a three-dimensional docking model of the TIR-TIR interaction between MyD88 and IL1RAcP PMID: 15849357
IL-1ra is associated with preserved beta-cell capacity in type 1 diabetes. PMID: 18299313
an isoform of the IL-1 receptor accessory protein (termed AcPb) was identified that is expressed exclusively in the CNS. PMID: 19481478

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Subcellular Location

[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.; [Isoform 3]: Secreted.

Protein Families

Interleukin-1 receptor family

Tissue Specificity

Detected in liver, skin, placenta, thymus and lung. Isoform 4 is predominantly expressed in brain. Overexpressed on candidate chronic myeloid leukemia (CML) stem cells, hematopoietic stem cells and mononuclear cells of patients with acute myeloid leukemia

Database Links

HGNC: 5995

OMIM: 602626

KEGG: hsa:3556

UniGene: Hs.478673

Code	CSB-MP878844HU
Abbreviation	Recombinant Human IL1RAP protein, partial (Active)
MSDS	MSDS Datasheet
Size	$186
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human IL1RAP at 2 μg/ml can bind Anti-IL1RAP recombinant antibody (CSB-RA878844MA1HU). The EC₅₀ is 0.9667-1.477 ng/mL. Biological Activity Assay The purity of IL1RAP was greater than 95% as determined by SEC-HPLC
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Recombinant Human Interleukin-1 receptor accessory protein (IL1RAP), partial (Active)

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