This recombinant human IL12RB1 protein is an active, high-quality product expressed in mammalian cells to preserve native conformation and post-translational modifications. Spanning amino acids 24 to 540 of the human IL12RB1 sequence, it is engineered with a C-terminal 10xHis tag for straightforward purification and detection. Supplied as a lyophilized powder, the recombinant IL12RB1 protein boasts a purity exceeding 95%, verified independently by both SDS-PAGE and SEC-HPLC. Endotoxin levels are tightly controlled, measuring under 1.0 EU/µg as confirmed by LAL testing. Functional performance was validated in ELISA assays, where the immobilized protein at 2 μg/mL effectively binds the anti-IL12RB1 recombinant antibody (CSB-RA011588MA1HU), with an EC50 between 1.578 and 2.143 ng/mL. These characteristics confirm the IL12RB1 protein's reliability and functionality in immunological or receptor-binding research applications.
IL12RB1 serves a pivotal function in the immune system by mediating the effects of IL-12, which is crucial for the differentiation of T cells into a type 1 helper (Th1) cell response. This receptor subunit, alongside its partner IL12RB2, forms a heterodimer that constitutes the high-affinity receptor for IL-12 on T and natural killer (NK) cells [1][2][3]. The binding of IL-12 to its receptor initiates a cascade of intracellular signals that are essential for producing the proinflammatory cytokine interferon-gamma (IFN-γ), thereby shaping the body's immune response towards a Th1 profile, which is essential for combating intracellular pathogens, such as Mycobacterium tuberculosis and Listeria monocytogenes [4][5].
Mutations or polymorphisms in the IL12RB1 gene can lead to significant immunological consequences. Homozygous recessive mutations obstruct the surface expression of the IL-12 receptor, which results in compromised IFN-γ secretion from T and NK cells, thereby impairing the immune response against various infections [1][6]. For instance, individuals with IL-12Rβ1 deficiency display an increased susceptibility to mycobacterial infections, underscoring the critical role of this receptor in protective immunity [7][6]. A study by Beaucoudrey et al. highlighted the association between gene mutations in IL12RB1 and increased vulnerability to non-tuberculous mycobacterial infections, illustrating the pivotal role of IL-12 signaling in host defense mechanisms [6].
The overall functionality of IL12RB1 is also highlighted in various genetic studies, indicating that different polymorphisms can be linked to autoimmune and infectious diseases, such as rheumatoid arthritis, tuberculosis, and even severe acute respiratory syndrome [2][8]. The relationship between IL-12 receptor signaling and Th1-mediated immune responses further emphasizes the importance of IL12RB1 in maintaining immune system balance and responding to infections effectively [3][9].
Furthermore, IL-12 not only stimulates cellular immunity but also has a role in linking innate and adaptive immune responses, making the IL-12/IL-12R axis fundamental in immunotherapy approaches as well as vaccine strategies. It has been shown that therapeutic applications leveraging the IL-12 pathway can enhance immune responses against various malignancies and infections, thereby highlighting the therapeutic potential of modulating this pathway [5][10].
References:
[1] K. Kusuhara, K. Yamamoto, K. Okada, Y. Mizuno, & T. Hara. Association of il12rb1 polymorphisms with susceptibility to and severity of tuberculosis in japanese: a gene‐based association analysis of 21 candidate genes. International Journal of Immunogenetics, vol. 34, no. 1, p. 35-44, 2007. https://doi.org/10.1111/j.1744-313x.2007.00653.x
[2] T. Tabone and G. Morahan. Definition of polymorphisms in the gene encoding the interleukin-12 receptor b1 subunit: testing linkage disequilibrium with type i diabetes susceptibility. Genes and Immunity, vol. 4, no. 3, p. 222-227, 2003. https://doi.org/10.1038/sj.gene.6363948
[3] B. Brattinga, A. Rutgers, et al. Preoperative inflammatory markers as a predictor of three-year overall survival in older cancer patients undergoing oncologic surgery. Cancers, vol. 13, no. 8, p. 1824, 2021. https://doi.org/10.3390/cancers13081824
[4] G. Orozco, M. González‐Gay, et al. Interleukin 12 (il12b) and interleukin 12 receptor (il12rb1) gene polymorphisms in rheumatoid arthritis. Human Immunology, vol. 66, no. 6, p. 710-714, 2005. https://doi.org/10.1016/j.humimm.2005.02.004
[5] G. Davis, L. Pfeiffer, D. Hemenway, & M. Rincón. Interleukin-12 is not essential for silicosis in mice. Particle and Fibre Toxicology, vol. 3, no. 1, 2006. https://doi.org/10.1186/1743-8977-3-2
[6] L. Beaucoudrey, A. Samarina, et al. Revisiting human il-12rβ1 deficiency Medicine, vol. 89, no. 6, p. 381-402, 2010. https://doi.org/10.1097/md.0b013e3181fdd832
[7] T. Sakai, M. Matsuoka, M. Aoki, K. Nosaka, & H. Mitsuya Missense mutation of the interleukin-12 receptor β1 chain–encoding gene is associated with impaired immunity againstmycobacterium avium complex infection. Blood, vol. 97, no. 9, p. 2688-2694, 2001. https://doi.org/10.1182/blood.v97.9.2688
[8] F. Tang, W. Liu, et al. Il-12 rb1 genetic variants contribute to human susceptibility to severe acute respiratory syndrome infection among chinese Plos One, vol. 3, no. 5, p. e2183, 2008. https://doi.org/10.1371/journal.pone.0002183
[9] L. Zhang, D. Prather, et al. Polymorphisms in genes of interleukin 12 and its receptors and their association with protection against severe malarial anaemia in children in western kenya. Malaria Journal, vol. 9, no. 1, 2010. https://doi.org/10.1186/1475-2875-9-87
[10] Y. Liu, N. Egilmez, & M. Russell. Enhancement of adaptive immunity to neisseria gonorrhoeae by local intravaginal administration of microencapsulated interleukin 12. The Journal of Infectious Diseases, vol. 208, no. 11, p. 1821-1829, 2013. https://doi.org/10.1093/infdis/jit354
