Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human SIGLEC9 at 2 μg/mL can bind Anti-SIGLEC9 recombinant antibody (CSB-RA021303MA1HU). The EC50 is 1.033-1.429 ng/mL.

Target Names

SIGLEC9

Uniprot No.

Q9Y336

Alternative Names

CDw329; Protein FOAP-9; CD329

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

18-348aa

Target Protein Sequence

QTSKLLTMQSSVTVQEGLCVHVPCSFSYPSHGWIYPGPVVHGYWFREGANTDQDAPVATNNPARAVWEETRDRFHLLGDPHTKNCTLSIRDARRSDAGRYFFRMEKGSIKWNYKHHRLSVNVTALTHRPNILIPGTLESGCPQNLTCSVPWACEQGTPPMISWIGTSVSPLDPSTTRSSVLTLIPQPQDHGTSLTCQVTFPGASVTTNKTVHLNVSYPPQNLTMTVFQGDGTVSTVLGNGSSLSLPEGQSLRLVCAVDAVDSNPPARLSLSWRGLTLCPSQPSNPGVLELPWVHLRDAAEFTCRAQNPLGSQQVYLNVSLQSKATSGVTQG

Mol. Weight

37.5 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

SIGLEC9 mediates immune inhibitory signaling through recognition of sialylated glycans, making it a critical checkpoint in myeloid cell regulation and tumor immune evasion. This recombinant extracellular domain (aa 18–348) demonstrates quantifiable antibody-binding activity with an EC50 of 1.033–1.429 ng/mL in functional ELISA, providing a validated reagent for therapeutic antibody epitope mapping, competitive inhibition screening, and blocking antibody development targeting SIGLEC9-sialoglycan interactions. Mammalian expression preserves the native glycosylation and disulfide architecture essential for authentic ligand recognition, supporting high-confidence data in surface plasmon resonance affinity characterization, biolayer interferometry kinetic studies, and small-molecule inhibitor screening campaigns. The protein meets the purity and endotoxin criteria typical for cell-based assays (>95% pure, <1.0 EU/μg), enabling its use as a positive control in binding platforms and in immune checkpoint research focused on myeloid immunomodulation.

Code	CSB-MP021303HU
Abbreviation	Recombinant Human SIGLEC9 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$138
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human SIGLEC9 at 2 μg/ml can bind Anti-SIGLEC9 recombinant antibody (CSB-RA021303MA1HU). The EC₅₀ is 1.033-1.429 ng/mL. Biological Activity Assay
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Recombinant Human Sialic acid-binding Ig-like lectin 9 (SIGLEC9), partial (Active)

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