Product Details

Purity

≥ 95% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human TM4SF1 at 5 μg/mL can bind Anti-TM4SF1 recombinant antibody(CSB-RA023615MA1HU). The EC50 is 4.079-4.472 ng/mL.The VLPs (CSB-MP3838) is negative control.

Target Names

TM4SF1

Uniprot No.

P30408

Alternative Names

M3S1;TAAL6

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

1-202aa

Target Protein Sequence

MCYGKCARCIGHSLVGLALLCIAANILLYFPNGETKYASENHLSRFVWFFSGIVGGGLLMLLPAFVFIGLEQDDCCGCCGHENCGKRCAMLSSVLAALIGIAGSGYCVIVAALGLAEGPLCLDSLGQWNYTFASTEGQYLLDTSTWSECTEPKHIVEWNVSLFSILLALGGIEFILCLIQVINGVLGGICGFCCSHQQQYDC

Mol. Weight

23 kDa

Protein Length

Full Length

Tag Info

C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

TM4SF1, a tetraspanin family member implicated in tumor progression and metastasis, is presented here as a full-length construct (aa 1–202) displayed on virus-like particles to preserve native membrane topology and conformational epitopes. Functional ELISA validation demonstrates robust antibody binding with an EC50 of 4.079–4.472 ng/mL when immobilized at 5 μg/mL, confirming that the VLP format maintains immunologically relevant surface presentation. This quantitative binding profile supports use in antibody screening campaigns, epitope mapping studies, and as a positive control in immunoassays targeting TM4SF1, while matched negative control VLPs enable rigorous specificity testing. Endotoxin levels below 1.0 EU/μg align with standards expected in cell-based binding assays and immunization protocols where low pyrogen content is critical.

Target Background

Gene References into Functions

our study provides a novel regulatory pathway involving TM4SF1, DDR1, MMP2 and MMP9, which promotes the formation and function of invadopodia to support cell migration and invasion in pancreatic cancer. PMID: 28368050
Regulation of transmembrane-4-L-six-family-1 (TM4SF1) on bladder cancer cell could be induced by peroxisome proliferator-activated receptor gamma (PPARgamma)-sirtuin 1 (SIRT1) feedback loop. PMID: 29175458
TM4SF1 was recognized as a direct target for miR-520f in hepatocellular carcinoma (HCC) cells where its expression was found up-regulated and inversely correlated with that of mir-520f. PMID: 29505836
Results suggest that miR-30a is an important regulator of TM4SF1, VEGF, and E-cadherin for CRC lymph node metastasis, a potential new therapeutic target in CRC. PMID: 28528497
High TM4SF1 expression is associated with esophageal cancer. PMID: 27974706
Replacement of the transmembrane 4 L six family protein TM4SF1 or TM4SF4 C-terminus with that of TM4SF5 increased spheroids growth, transwell migration, and invasive dissemination from spheroids in 3D collagen gels. PMID: 28129652
Results indicate that the expression of transmembrane 4 L6 family member 1 (TM4SF1) is higher in pancreatic cancer tissues and pancreatic cancer cell lines than controls. PMID: 27459514
Results show that TM4SF1 expression is elevated in colorectal cancer (CRC), and associated with tumor stage and lymph node metastasis. Also, miR-9 directly targeted its binding site in the TM4SF1 3'-UTR, which has a critical role in regulating CRC cell migration. and invasion. Furthermore, miR-9 regulated cell motility via suppressing PMID: 26983891
The findings suggest that TM4SF1 is a surface membrane antigen that is highly expressed in pancreatic cancer cells and increases the chemoresistance to gemcitabine. TM4SF1 may be a promising target to overcome the chemoresistance of pancreatic cancer. PMID: 26709920
TM4SF1 overexpression significantly contributed MDA-MB-231 cell migration but decreased apoptotic cells PMID: 26464650
Study shows that TM4SF1 expression is associated with better prognosis in pancreatic cancer. Its loss contributes to the invasion and migration of pancreatic cancer cells. PMID: 26035794
We found that miR-203 was significantly downregulated in OSF tissues compared to that in normal buccal mucosa tissues, and that miR-203 negatively regulated secreted SFRP4 and positively regulated TM4SF1 PMID: 25872484
Transmembrane-4-L-six-family-1 is overexpressed in human gliomas in general and the precise level of expression might predict outcome and could be of clinical value. PMID: 25855954
These findings validate TM4SF1 as an attractive candidate for cancer therapy with antibody-bound toxins that have the capacity to react with either cytoplasmic or nuclear targets in tumor cells or tumor-associated vascular endothelium. PMID: 26241677
TM4SF1 is a small plasma membrane glycoprotein that regulates cell motility and proliferation, and possibly a new vascular therapeutic target in cancer PMID: 24986520
High TM4SF1 expression is associated with pancreatic cancer. PMID: 24285464
TM4SF1, like genuine tetraspanins, serves as a molecular organizer that interacts with membrane and cytoskeleton-associated proteins and uniquely initiates the formation of nanopodia and facilitates cell polarization and migration. PMID: 21626280
We provide evidence that ARHGDIA, COBLL1, and TM4SF1 are negative regulators of apoptosis in cultured tumor cells. PMID: 21569526
Inhibition of cell migration after targeted knockdown of TM4SF1 protein expression suggests its contribution to prostate cancer cell metastasis. PMID: 21656834
TM4SF1 can serve as a surface protein marker which singly identifies MSCs from diverse cell sources, in particular, fibroblast-rich connective tissues PMID: 20486778
TAL6 may play a role in cancer invasion and metastasis PMID: 12855661
These data suggest that L6-Ag influences cell motility via TERM by regulating the surface presentation and endocytosis of some of their components. PMID: 18270265
The gene ratio test with the TM4SF1 gene for survival of patients with malignant pleural mesothelioma has robust predictive value. PMID: 19401544

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Subcellular Location

Membrane; Multi-pass membrane protein.

Protein Families

L6 tetraspanin family

Tissue Specificity

Highly expressed in lung, breast, colon and ovarian carcinomas. It is also present on some normal cells, endothelial cells in particular.

Database Links

HGNC: 11853

OMIM: 191155

KEGG: hsa:4071

STRING: 9606.ENSP00000304277

UniGene: Hs.351316

Code	CSB-MP023615HU
Abbreviation	Recombinant Human TM4SF1 protein-VLPs (Active)
MSDS	MSDS Datasheet
Size	$558
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Image	CSB-MP023615HU is detected by Mouse anti-6*His monoclonal antibody.The two bands respectively correspond to monomer, Homodimer. Activity Measured by its binding ability in a functional ELISA. Immobilized Human TM4SF1 at 5μg/mL can bind Anti-TM4SF1 recombinant antibody(CSB-RA023615MA1HU)， the EC₅₀ is 4.079-4.472 ng/mL.VLPs (CSB-MP3838) is negative control. Biological Activity Assay The purity of VLPs was greater than 95% as determined by SEC-HPLC.
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Recombinant Human Transmembrane 4 L6 family member 1(TM4SF1)-VLPs (Active)

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Target Background

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