Recombinant Human Trefoil factor 1 protein(TFF1) (Active)

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Code CSB-AP000341HU
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Product Details

Purity >97% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 μg/ml, corresponding to a specific activity of >100 IU/mg.
Target Names TFF1
Uniprot No. P04155
Research Area Signal Transduction
Alternative Names BCEI; Breast cancer estrogen inducible protein; Breast cancer estrogen inducible sequence; Breast cancer estrogen-inducible protein; D21S21; Gastrointestinal trefoil protein; Gastrointestinal trefoil protein pS2; hP1.A; HP1A; HPS 2; HPS2; pNR 2; PNR-2; pNR2; Polypeptide P1.A; Protein pS2; PS 2; pS2; pS2 protein; TFF 1; TFF1; TFF1_HUMAN; Trefoil factor 1
Species Homo sapiens (Human)
Source E.coli
Expression Region 25-84aa
Mol. Weight 6.7 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.
Gene References into Functions
  1. Findings suggest that the hypoxic conditions, which can be induced by gastric injury, promote TFF1 up-regulation, strengthened by an auto-induction mechanism, and that the trefoil peptide takes part in the epithelial-mesenchymal transition events eventually triggered to repair the damage. PMID: 29997345
  2. The urine TFF1 and TFF3 levels significantly increased with the progression of chronic kidney disease stages, but not the urine TFF2 levels. PMID: 29850501
  3. Study shows that TFF1 expression is silenced in early phases of esophageal squamous cell carcinoma (ESCC) development, which seems to be mediated at least in part by promoter hypermethylation, and provides the basis for the use of TFF1 expression as a potential biomarker for early ESCC detection. PMID: 29296124
  4. these data suggest that TFF1 might help cells to counteract Helicobacter colonization and the development of a chronic inflammation. PMID: 29085807
  5. Co-expression of GKN2 and TFF1 massively suppressed the expression of positive cell cycle regulators and induced G1/S arrest, synergistically enhancing the inhibition of cell viability and proliferation of gastric cancer cells PMID: 28150071
  6. The data suggest that the tumor suppressor activity of both RUNX1t1 and TFF1 are mechanistically connected to CEBPB and that cross-regulation between CEBPB-RUNX1t1-TFF1 plays an important role in gastric carcinogenesis. PMID: 27522676
  7. Low TFF1 expression is associated with retinoblastoma. PMID: 28481041
  8. The results suggest that the analysis of expression of MUC5AC and TFF1 may be useful for differentiating sessile serrated adenomas/polyps (SSA/Ps) from hyperplastic polyps (HPs). PMID: 28430953
  9. TFF1 displays a distinct protein expression pattern in the developing of airway remodeling due to Sulfur mustard inhalation and plays an important role in maintaining the airway epithelium function PMID: 27921407
  10. we found TFF1 plays an oncogenic role in mucinous ovarian cancer PMID: 26617749
  11. These results show increased levels of TFF1 and TFF3 in Chronic Kidney Disease patients with a pronounced elevation of urinary TFF1 in lower Chronic Kidney Disease stages. PMID: 26390128
  12. Loss of TFF1 could be a critical step in promoting the H. pylori-mediated oncogenic activation of beta-catenin and gastric tumorigenesis. PMID: 25980439
  13. We have established a novel, three-protein biomarker panel that is able to detect patients with early-stage pancreatic cancer in urine specimens:LYVE-1, REG1A, and TFF1 were selected as candidate biomarkers PMID: 26240291
  14. These findings suggest that the rs184432 and rs225359 polymorphisms in TFF1 have protective effects for GC and contribute to the development of GC in Korean individuals. PMID: 26240479
  15. Findings underscore an important protective role of TFF1 in abrogating H. pylori-mediated inflammation, a crucial hallmark of gastric tumorigenesis. Loss of TFF1 expression could be an important step in H. pylori-mediated gastric carcinogenesis. PMID: 26372254
  16. This study provides a potent tool of rTFF1 production to be applied in gastric damage protection and wound healing. The protein sources from B. choshinensis were more efficient than rTFF1 produced by E. coli PMID: 25990322
  17. trefoil factor 1 demonstrated breast specificity but was inferior to mammaglobin and GATA protein binding 3 PMID: 26276775
  18. Data indicate that microRNA miR218-5p negatively regulates trefoil factor 1 (TFF1) in an extracellular signal-regulated kinases Erk1/2-dependent manner. PMID: 25652124
  19. Trefoil Factor 1 levels can influence copper excretion and copper related toxicity. PMID: 25486181
  20. data clearly show that the expression of TFF3 in retinoblastoma cell lines is epigenetically regulated, whereas the level of TFF1 and TFF2 seems to be regulated by other or additional mechanisms PMID: 25199519
  21. Western blot analysis data demonstrated that miR-504 abrogates TFF1-induced p53 protein expression and activity. PMID: 25015107
  22. overexpressed in chronic rhinosinusitis without nasal polyps PMID: 24733740
  23. Immunohistochemical expression of ERalpha, ERbeta, and TFF1 in type I and II ovarian tumors. PMID: 24162262
  24. Determination of TFF1 status might identify patients at different risk for relapse and help in making decision on administering adjuvant therapy for early breast cancer patients during the first 3 years of follow-up. PMID: 24843314
  25. Retinoblastoma cell lines with high TFF1 expression levels exhibited a selective down-regulation of cyclin-dependent kinase (CDK) 6, whereas CDK4 and CDK2 seem to be unaffected by TFF1 expression. PMID: 22983508
  26. Piezo1 is a novel TFF1 binding protein that is important for TFF1-mediated cell migration in gastric cancer cells. PMID: 24798994
  27. the C-terminus of TFF1, involved in the binding of copper, is also able to selectively bind H. pylori RF-LPS. PMID: 24236136
  28. High TFF1 expression is associated with doxorubicininduced apoptosis resistance. PMID: 23836323
  29. miRNA-423-5p negatively regulated the expression of TFF1 by binding to its 3'UTR and participated in proliferation/invasion-related processes via a TFF1-dependent manner in gastric cancer cells PMID: 24486742
  30. TFF1 missense mutations seem to be a rare event in colorectal carcinogenesis. However, serum TFF1 levels were significantly elevated in patients with colorectal carcinoma. Serum TFF1 may be a potential useful marker for patients with colorectal carcinoma. PMID: 24503794
  31. Analysis of TFF1 levels dynamics revealed that TFF1 levels kept steady state during the 5-day period. TFF1 levels were similar in patients with SIRS, sepsis and severe sepsis PMID: 23627615
  32. MSK1 and MSK2 are required for maximal TFF 1 induction. PMID: 23675462
  33. downregulation of TFF1 expression during gastric neoplastic transformation is associated with activation of the NF-kappaB pathway PMID: 23942618
  34. TFF1 expression is silenced by DNA methylation and is associated with tumor invasion and a poor survival in gastric cancer patients. PMID: 23291975
  35. Reduced expression of TFF1 and increased expression of TFF3 may play a role in the carcinogenesis of gastric cancer. PMID: 23329884
  36. There is no direct molecular interaction between GKN1 and TFF1 in protein level in gastric cancer. PMID: 22842654
  37. The capacity of TFF1 to enhance prostate cancer cell migration/invasion is mediated by transcriptional repression of E-CADHERIN. PMID: 23266572
  38. The expression of TFF1 protein increases in gastric ulcer but decreases in gastric ulcerocancer. PMID: 22944057
  39. Report TFF1 expression in normal/diseased pancreas. PMID: 22286382
  40. Over-expression of TFF1 could promote apoptosis of three cell lines and inhibit proliferation but had no pronounced effect on cell cycle PMID: 22642590
  41. gastrointestinal tract specific gene GDDR might inhibit gastric cancer growth in a TFF1 dependent manner. PMID: 21870107
  42. PIMT may act as a co-activator in ERalpha-mediated transcription of TFF1 through its recruitment to the promoter via interacting with ERalpha. PMID: 22382029
  43. TFF1 promoter methylation is identified as a potential early marker of esophageal squamous cell carcinoma (ESCC). PMID: 21946330
  44. We found a statistically significant increased risk of gastric cancer associated with -394 TT genotypes PMID: 22093877
  45. documented the extent and timing of TFF1 expression in pancreatic ductal adenocarcinoma PMID: 21747314
  46. expression of TFF1 stimulated the migration and invasion in the four cell lines. Forced TFF1 expression in MCF10A, MDA-MB-231 and MCF7 cells did not modify anchorage-dependent or -independent cell proliferation PMID: 21358676
  47. The TFF1 promoter is activated by serum depletion according to promoter reporter assays in HEK 293 cells. PMID: 20494980
  48. TFF mRNA/protein expression is indicative of cholangiocarcinoma progression, but not predictive for histological sub-type or survival time. PMID: 21472131
  49. Data show that genes overexpressed in breast carcinoma including TFF1, TFF3, FOXA1 and CA12. PMID: 20132413
  50. loss of TFF1 leads to activation of IKK complex-regulated NF-kappaB transcription factors and is an important event in shaping the NF-kappaB-mediated inflammatory response during the progression to gastric tumorigenesis PMID: 21490402
  51. histone modifications governing TFF1 transcription are mediated by estrogen receptor PMID: 21378170
  52. TFF1 as a soluble factor designed to act mainly to antagonize the OIS process to accelerate tumorigenesis. PMID: 21451135
  53. Initiation of antral gastric cancer is associated with progressive epigenetic silencing of TFF1, which can be suppressed by the hormone gastrin. PMID: 21111741
  54. TFF1, TFF2, and PDX1 were expressed only in lobular endocervical glandular hyperplasia. PMID: 21228366
  55. MBD2 and ERalpha drive opposite effects on pS2 expression, which are associated with specific steady state levels of histone H3 acetylation and methylation marks PMID: 20300195
  56. Novel mutation in HPS2 causing Hermansky-Pudlak Syndrome Type 2. PMID: 20981699
  57. Overexpression and promoter hypomethylation of TFF1 is associated with prostate cancer. PMID: 20112343
  58. Cys58 and at least three Glu surrounding residues surrounding it, are essential to efficiently bind copper. Moreover, copper binding promotes the TFF1 homodimerization, thus increasing its motogenic activity in in vitro wound healing assays. PMID: 20213275
  59. TFF1, TFF2, and TFF3 expression were localized systematically in surgical specimens from the urinary tract. PMID: 20063012
  60. Specific DNA-protein interaction may explain the TFF peptides' cell specific expression pattern and altered levels in pathological conditions. PMID: 9855711
  61. The trefoil factor 1 participates in gastrointestinal cell differentiation by delaying G1-S phase transition and reducing apoptosis. PMID: 12034770
  62. Loss of heterozygosity and promoter methylation, but not mutation, may underlie loss in gastric carcinoma PMID: 12379766
  63. Expressed in conjunctiva in goblet cells. Review. PMID: 12613926
  64. Decreased TFF1 expression may lead to increased cell proliferation and then to the invasive character of intrahepatic cholangiocarcinoma. PMID: 14563942
  65. TFF1 may play an as yet undetermined role in the tumorigenesis of colorectal carcinomas. PMID: 14756547
  66. increased TFF1 and 2 concentrations found in serum from inflammatory bowel disease patients PMID: 15115253
  67. H. pylori interacts avidly with the dimeric form of TFF1, and this interaction enables binding to gastric mucin, suggesting that TFF1 may act as a receptor for the organism in vivo PMID: 15123808
  68. TFF1 is associated with the cell membrane of MCF-7 cells and is not linked via a GPI anchor. PMID: 15177868
  69. Sp1 or Sp3 aid in recruitment of HDACs and histone acetyltransferases (HATs) to mediate dynamic acetylation of histones associated with the TFF1 promoter PMID: 15541729
  70. The group of trefoil factor peptides (TFF1-3) are part of the protective mechanism operating in the intestinal mucosa and play a fundamental role in epithelial protection, repair, and restitution. PMID: 15578191
  71. trefoil factor family protein 1(TFF1)is expressed in inflamed non-neoplastic gallbladder epithelium and in low stage and low grade gallbladder carcinomas PMID: 15821928
  72. Decreased expression of TFF1 in carcinoma and its relativity to the differentiation suggests that TFF1 is related to gland and cell destruction of carcinoma. PMID: 15849833
  73. This is the first demonstration that a member of the trefoil factor family of proteins, TFF1, is bound covalently to a brichos domain-containing protein, TFIZ1; this has profound implications for TFF1 function. PMID: 15924415
  74. TFF-1 is lost late in the progression from Barrett's Esophagus to adenocarcinoma, while CES-2 is upregulated PMID: 15967118
  75. MUC5AC and MUC2, TFF1 and TFF3, APC and p21 in subsets of colorectal polyps and cancers suggests a distinct pathway of pathogenesis of mucinous carcinoma of the colorectum PMID: 16142311
  76. TFF1, TFF2, TFF3 and MUC5AC may have roles in pathogenesis of pterygium goblet cells PMID: 16142316
  77. urinary TFF1 is a CaOx crystal growth inhibitor with a potential pathophysiological role in nephrolithiasis PMID: 16308573
  78. TFF1 showed a statistically significant difference between early-onset gastric cancers and conventional gastric cancers. PMID: 16474375
  79. TFF1 and Cdc25A-B cooperate with other dominant oncogenic pathways to induce the adenoma and adenocarcinoma transitions. PMID: 16715141
  80. reduced expression of TFF1 and TFF2 in precancerous conditions and gastric cancer may be associated with the proliferation and malignant transformation of gastric mucosa PMID: 16718800
  81. D21S1893-D21S1890 region may harbor candidate genes especially TFF (TFF1, TFF2, and TFF3) and serine protease family, which might be involved in tumor invasion and metastasis contributing to poor survival. PMID: 16830362
  82. SET-mediated promoter hypoacetylation is a prerequisite for coactivation of the estrogen-responsive pS2 gene by PRMT1 PMID: 16861234
  83. Weaker expression of TFF1 in poorly-differentiated carcinoma may be related to the destruction of glands and cells in cancer tissues and the decrease in secretion of TFF1. PMID: 17143957
  84. IGF-1 induces phosphorylation and an increase of the AP1 complex, which is phosphorylated and binds to the pS2/TFF1 promoter, allowing recruitment of the chromatin remodeling factor Brg1 followed by binding of ERalpha via its interaction with c-Jun PMID: 17317669
  85. reveal a new property of TFF1 and suggest that copper could influence its biological activities by interfering with the dimerization of the peptide and/or the interaction with mucins or putative TFF receptors. PMID: 17610997
  86. The effect of plant-derived and synthetic estrogens on estrogen receptor alpha medicated cell proliferation and estrogen-regulated pS2 expression in MCF-2 cells are reported. PMID: 17651883
  87. The TFF1-GKN2 heterodimer and TFF2 differ characteristically by their binding to gastric mucins. PMID: 17982272
  88. Periodic, strand-specific methylation/demethylation occurs during transcriptional cycling of the pS2/TFF1 gene promoter on activation by oestrogens PMID: 18322525
  89. TFF was present in term and preterm human milk with rapidly declining concentrations during the first weeks post partum. PMID: 18502057
  90. TFF1 protects Chang conjunctival cells from apoptosis induced by UV irradiation and benzalkonium chloride, by preventing caspase-8 activation. PMID: 18515572
  91. Results describe the effects of estradiol on cyclin D1, PS2, and c-fos gene expression by measuring levels of RNA polymerase II on DNA templates, levels of nascent transcripts associated with RNA polymerase II, and levels of RNAs. PMID: 18644870
  92. These data suggest that highly active chromatin is not only permissive for transcription, but can override the requirement for the transcription factor at an inducible promoter. PMID: 18662686
  93. in gastric cancer, expression of TFF1 in the absence of TFIZ1 is associated with lymph node metastasis PMID: 18722547
  94. Breast cancer patients with pre-operative elevated bone marrow levels of Mammaglobin Aand/or trefoil factor 1 mRNA seem to constitute a small group of patients with a very poor prognosis. PMID: 18846421
  95. Decreased expression of TFF1 is associated with barrett's esophagus-associated high-grade dysplasia. PMID: 18927283
  96. High sensitivity of pS2 expression in prostate carcinoma could make it a suitable marker for diagnosis of prostate carcinoma, especially in metastatic cases of unknown origin. PMID: 19132993
  97. This study demonstrates the over-expression in CTSE, in particular, and TFF1 in sessile serrated adenomas compared to both hyperplastic polyps and tubular adenomas. PMID: 19172291
  98. circulating TFFs are not candidate markers of trisomy 21 in first-trimester pregnancies PMID: 19172695
  99. RNA interference-mediated depletion of TFF1 in mammary carcinoma cells significantly reduced anchorage-independent growth and migration. PMID: 19589871
  100. The study demonstrated a selective binding affinity of TFF1 for copper. PMID: 17610997

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Subcellular Location Secreted
Tissue Specificity Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level). Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal g
Database Links

HGNC: 11755

OMIM: 113710

KEGG: hsa:7031

STRING: 9606.ENSP00000291527

UniGene: Hs.162807

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