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| Code | CSB-MP897523HU1-B |
| Size | $498 |
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| Description |
The recombinant human TNFSF13B was produced in mammalian cells. The DNA fragment used to prepare the recombinant TNFSF13B protein corresponds to amino acid 134-285 of the human TNFSF13B protein containing an N-terminal hFc-Avi-tag. This TNFSF13B protein is an active protein, whose bio-activity has been validated in the functional ELISA. It binds to the human BCMA or human TNFRSF13C, with the EC50 of 0.1752-0.3657 ng/ml or 0.2699-0.5613 ng/ml, respectively. Its purity reaches up to 92%, determined by SDS-PAGE. This TNFSF13B protein has an apparent molecular mass of 46 kDa on the gel while its predicted mass is 46.2 kDa. Its endotoxin is less than 1.0 EU/ug measured by the LAL method. It is available now. TNFSF13B, also called BAFF, modulates B cell survival, maturation, and differentiation by binding to its receptors, BAFF-R, TACI, and BCMA. Upregulation of BAFF is related to autoimmune diseases, including systemic lupus erythematosus, rheumatoid arthritis, and multiple myeloma. BAFF/BAFFR signaling is involved in the regulation of protein synthesis and energy metabolism required to extend the half-life of immature, transitional, and mature B cells, as well as other survival functions. |
| Purity | Greater than 92% as determined by SDS-PAGE. |
| Endotoxin | Less than 1.0 EU/ug as determined by LAL method. |
| Activity | ①Measured by its binding ability in a functional ELISA. Immobilized human BCMA (CSB-MP023974HU1) at 5 μg/ml can bind Biotinylated human TNFSF13B, the EC50 is 0.1752-0.3657 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF13C (CSB-MP853495HU) at 2 μg/ml can bind Biotinylated human TNFSF13B, the EC50 is 0.2699-0.5613 ng/ml. |
| Target Names | TNFSF13B |
| Uniprot No. | Q9Y275 |
| Alternative Names |
B lymphocyte stimulator （BLyS） （B-cell-activating factor） （BAFF） （Dendritic cell-derived TNF-like molecule） （TNF- and APOL-related leukocyte expressed ligand 1） （TALL-1）
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| Molecular Characterization |  |
| Species | Homo sapiens (Human) |
| Source | Mammalian cell |
| Expression Region | 134-285aa |
| Target Protein Sequence | AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL |
| Mol. Weight | 46.2 kDa |
| Protein Length | Partial |
| Tag Info |
N-terminal hFc-Avi-tagged |
| Form |
Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4 |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 3-7 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.; Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.; Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis.
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| Gene References into Functions |
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| Subcellular Location | Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor ligand superfamily member 13b, soluble form]: Secreted. |
| Protein Families | Tumor necrosis factor family |
| Tissue Specificity | Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, |
| Database Links |
HGNC: 11929 OMIM: 603969 KEGG: hsa:10673 STRING: 9606.ENSP00000365048 UniGene: Hs.525157 |
