Purity
Greater than 90% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized human BCMA (CSB-MP023974HU1) at 5 μg/ml can bind Biotinylated human TNFSF13B, the EC50 is 0.1752-0.3657 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF13C (CSB-MP853495HU) at 2 μg/ml can bind Biotinylated human TNFSF13B, the EC50 is 0.2699-0.5613 ng/ml.
Alternative Names
B lymphocyte stimulator （BLyS） （B-cell-activating factor） （BAFF） （Dendritic cell-derived TNF-like molecule） （TNF- and APOL-related leukocyte expressed ligand 1） （TALL-1）
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
134-285aa
Target Protein Sequence
AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL
Tag Info
N-terminal hFc-Avi-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant human TNFSF13B was produced in mammalian cells. The DNA fragment used to prepare the recombinant TNFSF13B protein corresponds to amino acid 134-285 of the human TNFSF13B protein containing an N-terminal hFc-Avi-tag. This TNFSF13B protein is an active protein, whose bio-activity has been validated in the functional ELISA. It binds to the human BCMA or human TNFRSF13C, with the EC50 of 0.1752-0.3657 ng/ml or 0.2699-0.5613 ng/ml, respectively. Its purity reaches up to 90%, determined by SDS-PAGE. This TNFSF13B protein has an apparent molecular mass of 46 kDa on the gel while its predicted mass is 46.2 kDa. Its endotoxin is less than 1.0 EU/ug measured by the LAL method. It is available now.
TNFSF13B, also called BAFF, modulates B cell survival, maturation, and differentiation by binding to its receptors, BAFF-R, TACI, and BCMA. Upregulation of BAFF is related to autoimmune diseases, including systemic lupus erythematosus, rheumatoid arthritis, and multiple myeloma. BAFF/BAFFR signaling is involved in the regulation of protein synthesis and energy metabolism required to extend the half-life of immature, transitional, and mature B cells, as well as other survival functions.
