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| Code | CSB-MP897523HU1 |
| Size | $428 |
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| Description |
The active recombinant human tumor necrosis factor ligand superfamily member 13B (TNFSF13B) is expressed from mammalian cells, with an N-terminal hFc-tag. Its expression region maps within amino acid residues Ala134-Leu285 of the human TNFSF13B protein. This recombinant human TNFSF13B protein is characterized by high purity (>93%, SDS-PAGE), low endotoxin (<1.0 EU/ug protein, LAL method), and relatively high bioactivity. In the functional ELISA, the immobilized TNFSF13B can bind to the human BCMA or TNFRSF13C, with an EC50 constant of 221.3-298.6 ng/ml and 9.943-15.72 ng/ml, respectively. In the LSPR assay, the human TNFSF13B protein captured on the COOH chip can bind to the human BCMA, with an affinity constant of 39 nM. And it is in stock now. TNFSF13B, also known as BAFF, plays an important role in the proliferation and differentiation of B cells. It also influences antibody class switch. TNFSF13B is involved in the pathophysiology of pulmonary diseases. |
| Purity | Greater than 93% as determined by SDS-PAGE. |
| Endotoxin | Less than 1.0 EU/ug as determined by LAL method. |
| Activity | ①Measured by its binding ability in a functional ELISA. Immobilized TNFSF13B at 10 μg/ml can bind human BCMA (CSB-MP023974HU1), the EC50 of human TNFSF13B protein is 221.3-298.6 ng/ml.②Human TNFSF13B protein Fc tag (CSB-MP897523HU1) captured on COOH chip can bind Human BCMA protein Fc tag (CSB-MP023974HU1) with an affinity constant of 39 nM as detected by LSPR Assay.③Measured by its binding ability in a functional ELISA. Immobilized TNFSF13B at 2 μg/ml can bind TNFRSF13C(CSB-MP853495HU1), the EC50 is 9.943-15.72 ng/ml. |
| Target Names | TNFSF13B |
| Uniprot No. | Q9Y275 |
| Research Area | Cancer |
| Alternative Names | B lymphocyte stimulator (BLyS) (B-cell-activating factor) (BAFF) (Dendritic cell-derived TNF-like molecule) (TNF- and APOL-related leukocyte expressed ligand 1) (TALL-1) |
| Molecular Characterization |  |
| Species | Homo sapiens (Human) |
| Source | Mammalian cell |
| Expression Region | 134-285aa |
| Target Protein Sequence | AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL |
| Mol. Weight | 46.6 kDa |
| Protein Length | Partial |
| Tag Info |
N-terminal hFc-tagged |
| Form |
Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 3-7 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.; Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.; Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis.
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| Gene References into Functions |
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| Subcellular Location | Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor ligand superfamily member 13b, soluble form]: Secreted. |
| Protein Families | Tumor necrosis factor family |
| Tissue Specificity | Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, |
| Database Links |
HGNC: 11929 OMIM: 603969 KEGG: hsa:10673 STRING: 9606.ENSP00000365048 UniGene: Hs.525157 |
