Purity
Greater than 90% as determined by SDS-PAGE.
Greater than 90% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized TNFSF13B at 10 μg/ml can bind human BCMA (CSB-MP023974HU1), the EC50 of human TNFSF13B protein is 221.3-298.6 ng/ml.②Human TNFSF13B protein Fc tag (CSB-MP897523HU1) captured on COOH chip can bind Human BCMA protein Fc tag (CSB-MP023974HU1) with an affinity constant of 39 nM as detected by LSPR Assay.③Measured by its binding ability in a functional ELISA. Immobilized TNFSF13B at 2 μg/ml can bind TNFRSF13C(CSB-MP853495HU1), the EC50 is 9.943-15.72 ng/ml.
Alternative Names
B lymphocyte stimulator (BLyS) (B-cell-activating factor) (BAFF) (Dendritic cell-derived TNF-like molecule) (TNF- and APOL-related leukocyte expressed ligand 1) (TALL-1)
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
134-285aa
Target Protein Sequence
AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL
Tag Info
N-terminal hFc-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The active recombinant human tumor necrosis factor ligand superfamily member 13B (TNFSF13B) is expressed from mammalian cells, with an N-terminal hFc-tag. Its expression region maps within amino acid residues Ala134-Leu285 of the human TNFSF13B protein. This recombinant human TNFSF13B protein is characterized by high purity (>90%, SDS-PAGE), low endotoxin (<1.0 EU/ug protein, LAL method), and relatively high bioactivity. In the functional ELISA, the immobilized TNFSF13B can bind to the human BCMA or TNFRSF13C, with an EC50 constant of 221.3-298.6 ng/ml and 9.943-15.72 ng/ml, respectively. In the LSPR assay, the human TNFSF13B protein captured on the COOH chip can bind to the human BCMA, with an affinity constant of 39 nM. And it is in stock now.
TNFSF13B, also known as BAFF, plays an important role in the proliferation and differentiation of B cells. It also influences antibody class switch. TNFSF13B is involved in the pathophysiology of pulmonary diseases.