Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CD20 at 10 μg/mL can bind Anti-CD20 recombinant antibody (CSB-RA015007MA3HU), the EC50 is 10.65-15.26 ng/mL.The VLPs (CSB-MP3838) is negative control.

Target Names

MS4A1

Uniprot No.

M4ZHZ6

Alternative Names

Membrane spanning 4-domains A1; Membrane-spanning 4-domains subfamily A member 1; CD20

Molecular Characterization

Species

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Source

Mammalian cell

Expression Region

1-297aa

Target Protein Sequence

MTTPRNSVNGTFPAEPMKGPIAMQPGPKPLLRRMSSLVGPTQSFFMRESKALGAVQIMNGLFHIALGGLLMIPAGIYAPICVTVWYPLWGGIMYIISGSLLAATEKNSRKCLVKGKMIMNSLSLFAAISGMILSIMDILNIKISHFLKMESLNFIRVHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWRRTCSRPKSSVVLLSAEEKKEQVIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP

Mol. Weight

34.4 kDa

Protein Length

Full Length

Tag Info

C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CD20 (MS4A1) is a critical B-cell surface marker widely targeted in oncology antibody development, yet evaluating anti-CD20 candidates requires antigen presented in a native membrane context rather than as soluble ectodomain fragments. This recombinant cynomolgus macaque CD20 is displayed on virus-like particles (VLPs) as a full-length protein (aa 1–297) expressed in mammalian cells, preserving multi-pass transmembrane topology that soluble constructs cannot replicate. Functional ELISA confirms that immobilized VLP-CD20 at 10 µg/mL binds an anti-CD20 recombinant antibody with an EC₅₀ of 10.65–15.26 ng/mL, while control VLPs show no binding, supporting use in antibody screening, cross-reactivity profiling between human and cynomolgus targets, and preclinical binding assays critical for therapeutic antibody development. The C-terminal 10×His tag is accessible only under denaturing conditions, and endotoxin levels below 1.0 EU/µg satisfy the criteria typical for cell-based functional assays and in vivo bridging studies.

Code	CSB-MP4516MOV
Abbreviation	Recombinant Cynomolgus monkey MS4A1 protein-VLPs (Active)
MSDS	MSDS Datasheet
Size	$558
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Image	CSB-MP4516MOV is detected by Mouse anti-6*His monoclonal antibody. Activity Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CD20 at 10 μg/mL can bind Anti-CD20 recombinant antibody (CSB-RA015007MA3HU), the EC₅₀ is 10.65-15.26 ng/mL.The VLPs (CSB-MP3838) is negative control. Biological Activity Assay
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Recombinant Macaca fascicularis Membrane spanning 4-domains A1 (MS4A1)-VLPs (Active)

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