Purity

Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis PTK7 at 2 μg/mL can bind Anti-PTK7 recombinant antibody (CSB-RA622651MA1HU). The EC50 is 1.399 - 1.666 ng/mL.

Target Names

PTK7

Uniprot No.

A0A2K5VNI5

Species

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Source

Mammalian cell

Expression Region

39-712aa

Target Protein Sequence

AIVFIKQPSSQDALQGRRALLRCEVEAPGLVHVYWLLDGAPVQDTERRFTQGSSLSFAAVDRLQDSGTFQCVARDDVTGEEARSANASFNIKWIEAGPVVLKHPASEAEIQPQTQVTLRCHIDGHPRPTYQWFRDGTPLSDGQSNNTVSSKERNLTLRPAGPEHSGLYSCCAHNAFGQACSSQNFTLSIADESFARVVLAPQDVIVARNEEAMFHCQFSAQPPPNLQWLFEDETPITNRSRPPHLRRATVFANGSLLLTQVRPRNAGVYRCIGQGQRGPPVILEATLHLAEIEDMPLFEPRVFTAGSEERVTCLPPKGLPEPSVWWEHAGVRLPTHGRVYQKGHELVLASIAESDAGVYTCHAANLAGQRRQDVNITVATVPTWLKKPQDSQLEEGKPGYLHCLTQATPKPTVVWYRNQMLISEDSRFEVFKNGTLRINNVEVYDGTWYRCVSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTIKWERADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNGPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLQCEAQGDPKPLIQWKGKDRILDPTKLGPRMHIFQNGSLVIHDVAPEDSGRYTCIAGNSCNIKHTEAPLYVVDKPVPEESEGPGSPPPYKMIQT

Mol. Weight

76.1 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

PTK7 functions as a pseudokinase receptor implicated in Wnt signaling and cell polarity, making it a target of interest in developmental biology and oncology research. This cynomolgus macaque ortholog, spanning residues 39–712 and expressed in mammalian cells to preserve native glycosylation and disulfide architecture, demonstrates quantifiable antibody-binding activity with an EC50 of 1.399–1.666 ng/mL in functional ELISA, confirming that the extracellular domain retains conformational epitopes required for immunological recognition. The validated binding profile supports its use in therapeutic antibody epitope mapping, competitive inhibition screening to identify blocking candidates, and as a positive control in ELISA or surface plasmon resonance assays characterizing anti-PTK7 affinity reagents. Purity exceeding 95% by SDS-PAGE and endotoxin below 1.0 EU/μg align with standards expected in antibody development workflows and receptor-ligand interaction studies where low contaminant levels are critical for reproducible kinetic measurements.

Code	CSB-MP5478MOV
Abbreviation	Recombinant Cynomolgus monkey PTK7 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$9.9
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis PTK7 at 2 μg/ml can bind Anti-PTK7 recombinant antibody (CSB-RA622651MA1HU). The EC₅₀ is 1.399 - 1.666 ng/mL. Biological Activity Assay The purity of PTK7 was greater than 95% as determined by SEC-HPLC
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Recombinant Macaca fascicularis Inactive tyrosine-protein kinase 7 (PTK7), partial (Active)

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