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Recombinant Macaca fascicularis Transmembrane 4 L6 family member 1 (TM4SF1)-VLPs (Active)

Recombinant Macaca fascicularis Transmembrane 4 L6 family member 1 (TM4SF1)-VLPs (Active)

^{In Stock}

Code	CSB-MP5031MOV
Abbreviation	Recombinant Cynomolgus monkey TM4SF1 protein-VLPs (Active)
MSDS	MSDS Datasheet
Size	$620
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Image	CSB-MP5031MOV is detected by Mouse anti-6*His monoclonal antibody.The two bands respectively correspond to monomer, Homodimer. Activity Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus TM4SF1 at 5μg/mL can bind Anti-TM4SF1 recombinant antibody(CSB-RA023615MA1HU)， the EC₅₀ is 4.480-4.930 ng/mL.VLPs (CSB-MP3838) is negative control. Biological Activity Assay The purity of VLPs was greater than 95% as determined by SEC-HPLC TM4SF1 Recombinant Monoclonal Antibody (CSB-RA023615MA1HU) captured on Protein A Chip can bind Recombinant Cynomolgus monkey TM4SF1 protein-VLPs with an affinity constant of 0.833 nM as detected by MetaSPR Assay (WeSPRTM 200).
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Product Details
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Product Details

Purity

≥ 95% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus TM4SF1 at 5 μg/mL can bind Anti-TM4SF1 recombinant antibody(CSB-RA023615MA1HU).The EC50 is 4.480-4.930 ng/mL.The VLPs (CSB-MP3838) is negative control.

Target Names

TM4SF1

Uniprot No.

A0A2K5VKY2

Species

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Source

Mammalian cell

Expression Region

1-202aa

Target Protein Sequence

MCYGKCARCIGHSLVGLALLCIIANILLYFPNGETKYASENHLSRFVWFFSGIVGGGLLMLLPAFVFIGLEQDDCCGCCGHENCGKRCAMLSSVLAALIGIAGSGYCVIVAALGLAEGPLCLDSFGQWNYTFASTEGQYLLDTSTWSQCTEPKHIVEWNVSLFSILLALGGIEFILCLIQVINGVLGGICGFCCSRQQQYDC

Mol. Weight

23.1 kDa

Protein Length

Full Length

Tag Info

C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

TM4SF1, a tetraspanin family member implicated in tumor cell migration and metastasis, is presented here as a full-length construct (aa 1–202) displayed on virus-like particles to preserve native membrane topology and quaternary structure. Functional ELISA validation demonstrates specific antibody binding with an EC50 of 4.480–4.930 ng/mL when the protein is immobilized at 5 μg/mL, confirming that conformational epitopes remain accessible in this VLP format. This quantitative binding profile supports use in antibody screening campaigns, epitope mapping studies, and as a positive control in immunoassays targeting TM4SF1, while the matched negative control VLP (CSB-MP3838) enables rigorous specificity testing. Endotoxin levels below 1.0 EU/μg satisfy the contamination thresholds commonly required for cell-based binding assays and functional studies investigating TM4SF1's role in cancer cell adhesion and invasion.