Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse CD93 at 2 μg/mL can bind Mouse IGFBP7 (CSB-MP713980MO), the EC50 is 373.4-836.8 ng/mL.
Research Area
Cardiovascular
Alternative Names
(C1q/MBL/SPA receptor)(C1qR(p))(C1qRp)(Cell surface antigen AA4)(Complement component 1 q subcomponent receptor 1)(Lymphocyte antigen 68)(Ly-68)(CD antigen CD93)
Molecular Characterization
Species
Mus musculus (Mouse)
Expression Region
23-572aa
Target Protein Sequence
ADSQAVVCEGTACYTAHWGKLSAAEAQHRCNENGGNLATVKSEEEARHVQQALTQLLKTKAPLEAKMGKFWIGLQREKGNCTYHDLPMRGFSWVGGGEDTAYSNWYKASKSSCIFKRCVSLILDLSLTPHPSHLPKWHESPCGTPEAPGNSIEGFLCKFNFKGMCRPLALGGPGRVTYTTPFQATTSSLEAVPFASVANVACGDEAKSETHYFLCNEKTPGIFHWGSSGPLCVSPKFGCSFNNGGCQQDCFEGGDGSFRCGCRPGFRLLDDLVTCASRNPCSSNPCTGGGMCHSVPLSENYTCRCPSGYQLDSSQVHCVDIDECQDSPCAQDCVNTLGSFHCECWVGYQPSGPKEEACEDVDECAAANSPCAQGCINTDGSFYCSCKEGYIVSGEDSTQCEDIDECSDARGNPCDSLCFNTDGSFRCGCPPGWELAPNGVFCSRGTVFSELPARPPQKEDNDDRKESTMPPTEMPSSPSGSKDVSNRAQTTGLFVQSDIPTASVPLEIEIPSEVSDVWFELGTYLPTTSGHSKPTHEDSVSAHSDTDGQN
Tag Info
C-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
This Mouse CD93 recombinant protein was produced in mammalian cell, where the gene sequence encoding Mouse CD93 (23-572aa) was expressed with the C-terminal 10xHis tag. The purity of this CD93 protein was greater than 95% by SDS-PAGE. The activity was validated.
CD93 (C1qRP, AA4.1) is a C-type lectin-like domain (CTLD) protein. The CD93 gene is considered to be derived from the gene duplication of the group XIV member thrombomodulin (TM), so it is classified as the XIV group in transmembrane glycoprotein.
CD93 was originally thought to be a C1q receptor that enhances phagocytosis. Subsequent studies showed that in CD93-null mice, phagocytosis of C1q by macrophages is enhanced despite defective clearance of apoptotic cells in vivo, and thus CD93 is not a receptor for C1q and regulates phagocytosis through an unknown mechanism. The function of CD93 is currently mainly considered to be involved in intercellular adhesion and clearance of apoptotic cells, and is associated with various inflammatory and immune-related diseases, including asthma.
CD93 is a transmembrane receptor that is upregulated in tumor blood vessels in many cancers. Studies have demonstrated that CD93 regulates β1 integrin signaling activation and fibronectin fibrillation during tumor angiogenesis.
A recent study comparing gene expression profiling of tumors under VEGF inhibitor treatment in vivo identified CD93 as a candidate receptor downregulated in VEGF inhibition and a potential target for mediating vascular normalization. This confirms the pro-angiogenic effect of CD93 in endothelial cells.