Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Mouse CD93 at 2 μg/mL can bind Mouse IGFBP7 (CSB-MP713980MO), the EC50 is 373.4-836.8 ng/mL.

Target Names

Cd93

Uniprot No.

O89103

Research Area

Cardiovascular

Alternative Names

(C1q/MBL/SPA receptor)(C1qR(p))(C1qRp)(Cell surface antigen AA4)(Complement component 1 q subcomponent receptor 1)(Lymphocyte antigen 68)(Ly-68)(CD antigen CD93)

Molecular Characterization

Species

Mus musculus (Mouse)

Source

Mammalian cell

Expression Region

23-572aa

Target Protein Sequence

ADSQAVVCEGTACYTAHWGKLSAAEAQHRCNENGGNLATVKSEEEARHVQQALTQLLKTKAPLEAKMGKFWIGLQREKGNCTYHDLPMRGFSWVGGGEDTAYSNWYKASKSSCIFKRCVSLILDLSLTPHPSHLPKWHESPCGTPEAPGNSIEGFLCKFNFKGMCRPLALGGPGRVTYTTPFQATTSSLEAVPFASVANVACGDEAKSETHYFLCNEKTPGIFHWGSSGPLCVSPKFGCSFNNGGCQQDCFEGGDGSFRCGCRPGFRLLDDLVTCASRNPCSSNPCTGGGMCHSVPLSENYTCRCPSGYQLDSSQVHCVDIDECQDSPCAQDCVNTLGSFHCECWVGYQPSGPKEEACEDVDECAAANSPCAQGCINTDGSFYCSCKEGYIVSGEDSTQCEDIDECSDARGNPCDSLCFNTDGSFRCGCPPGWELAPNGVFCSRGTVFSELPARPPQKEDNDDRKESTMPPTEMPSSPSGSKDVSNRAQTTGLFVQSDIPTASVPLEIEIPSEVSDVWFELGTYLPTTSGHSKPTHEDSVSAHSDTDGQN

Mol. Weight

60.9 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This Mouse CD93 recombinant protein was produced in mammalian cell, where the gene sequence encoding Mouse CD93 (23-572aa) was expressed with the C-terminal 10xHis tag. The purity of this CD93 protein was greater than 95% by SDS-PAGE. The activity was validated.

CD93 (C1qRP, AA4.1) is a C-type lectin-like domain (CTLD) protein. The CD93 gene is considered to be derived from the gene duplication of the group XIV member thrombomodulin (TM), so it is classified as the XIV group in transmembrane glycoprotein.
CD93 was originally thought to be a C1q receptor that enhances phagocytosis. Subsequent studies showed that in CD93-null mice, phagocytosis of C1q by macrophages is enhanced despite defective clearance of apoptotic cells in vivo, and thus CD93 is not a receptor for C1q and regulates phagocytosis through an unknown mechanism. The function of CD93 is currently mainly considered to be involved in intercellular adhesion and clearance of apoptotic cells, and is associated with various inflammatory and immune-related diseases, including asthma.
CD93 is a transmembrane receptor that is upregulated in tumor blood vessels in many cancers. Studies have demonstrated that CD93 regulates β1 integrin signaling activation and fibronectin fibrillation during tumor angiogenesis.
A recent study comparing gene expression profiling of tumors under VEGF inhibitor treatment in vivo identified CD93 as a candidate receptor downregulated in VEGF inhibition and a potential target for mediating vascular normalization. This confirms the pro-angiogenic effect of CD93 in endothelial cells.

Target Background

Function

Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion. Marker for early multipotent hematopoietic precursor cells. May play a role in cell-cell interactions during hematopoietic and vascular development.

Gene References into Functions

serum CD93 is a biomarker for allergic asthma PMID: 29498549
The expression of cd93 was observed in pancreatic islets, and leaky vessels were apparent in cd93-deficient pancreases. We further demonstrated that stress-induced release of sCD93 is impaired by hyperglycemia. Study propose CD93 as an important component in glucometabolic regulation. PMID: 27659228
soluble EGF-like domain containing CD93 protein is a novel angiogenic factor acting on the endothelium PMID: 23272129
[review] CD93 regulates inflammation as demonstrated in a murine model of cerebral ischemia reperfusion-induced injury and peritonitis; recent data suggests that a soluble form of CD93 regulates the inflammatory function of monocytes/ macrophages. PMID: 22206251
these data suggest that cell-associated CD93 regulates leukocyte recruitment and complement activation during murine peritonitis. PMID: 21849679
we speculate that CD93-neuroprotection is mediated via suppression of the neuroinflammatory response through downregulation of CCL21. PMID: 20439917
Cd93 may be an autoimmune susceptibility gene residing within the Idd13 locus, which plays a role in regulating absolute numbers of CD4(+) NKT cells. PMID: 20387063
Data demonstrate that inflammation triggers release of sCD93 in vivo, identify the inflammatory macrophage as a source of sCD93, and provide insight into the mechanism by which CD93 contributes to engulfment of apoptotic cells. PMID: 19603257
Impaired uptake of apoptotic cells in C1qRp-deficient mice indicates that the C1qRp receptor may contribute to the removal of dying cells in vivo. PMID: 15004139
CD93-deficient mice were unable to maintain antibody secretion and bone-marrow plasma-cell numbers, demonstrating that CD93 is important for the maintenance of plasma cells in bone marrow niches. PMID: 19228948
These data suggest that AA4.1 is a cell surface marker that can identify the earliest lymphohematopoietic progenitors in mouse development. PMID: 19458045

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed in lung, heart and bone marrow. Expressed at lower level in ovary, whole embryo and fetal liver. Not detected in brain, adult liver or thymus. Highly expressed in peritoneal cavity and bone marrow macrophages. Not detected in epithelial cells.

Database Links

KEGG: mmu:17064

STRING: 10090.ENSMUSP00000096876

UniGene: Mm.681

Code	CSB-MP004970MO
Abbreviation	Recombinant Mouse Cd93 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$114
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Mouse CD93 at 2 μg/mL can bind Mouse IGFBP7 (CSB-MP713980MO), the EC₅₀ is 373.4-836.8 ng/mL.
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Recombinant Mouse Complement component C1q receptor (Cd93), partial (Active)

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