Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Mouse FGFR2 at 2 μg/mL can bind Anti-FGFR2 recombinant antibody (CSB-RA008645MA1HU). The EC50 is 2.084-2.249 ng/mL.

Target Names

Fgfr2

Uniprot No.

P21803-2

Alternative Names

FGFR-2; Keratinocyte growth factor receptor (KGFR); CD332; Fgfr2; Bek, Ect1

Species

Mus musculus (Mouse)

Source

Mammalian cell

Expression Region

39-263aa

Target Protein Sequence

PYWTNTEKMEKRLHACPAANTVKFRCPAGGNPTSTMRWLKNGKEFKQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCLVENEYGSINHTYHLDVVERSPHRPILQAGLPANASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNGSKNGPDGLPYLKVLKHSGINSSNAEVLALFNVTEMDAGEYICKVSNYIGQANQSAWLTVLPKQQAPVREKEITASPDYLE

Mol. Weight

26.6 kDa

Protein Length

Partial of Isoform Short

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

FGFR2 mediates critical signaling in development and tissue homeostasis, and its dysregulation drives oncogenesis in multiple epithelial cancers, making receptor-ligand interaction studies essential for therapeutic target validation. This mammalian-expressed construct spanning residues 39–263 of the short isoform captures the extracellular ligand-binding domain with native post-translational modifications that preserve conformational epitopes, as demonstrated by its specific binding to an anti-FGFR2 recombinant antibody with an EC50 of 2.084–2.249 ng/mL in functional ELISA. The quantified binding kinetics support its use in competitive inhibition assays for blocking antibody screening, therapeutic antibody epitope mapping, and small-molecule inhibitor discovery campaigns targeting the FGFR2 extracellular interface. Purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg meet the quality thresholds commonly required for surface plasmon resonance affinity characterization and biolayer interferometry studies where low background interference is critical.

Code	CSB-MP008645MO1(F2)
Abbreviation	Recombinant Mouse Fgfr2 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$118
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Mouse FGFR2 at 2 μg/ml can bind Anti-FGFR2 recombinant antibody (CSB-RA008645MA1HU). The EC₅₀ is 2.084-2.249 ng/mL. Biological Activity Assay
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Recombinant Mouse Fibroblast growth factor receptor 2 (Fgfr2), partial (Active)

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