Recombinant Mouse Glypican-1(Gpc1) (Active)

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Code CSB-AP005761MO
Size US$3267Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity The ED50 as determined by its ability to bind Human FGFb in functional ELISA is less than 10 ug/ml.
Target Names Gpc1
Uniprot No. Q9QZF2
Research Area Signal Transduction
Alternative Names Gpc1; Glypican-1 [Cleaved into: Secreted glypican-1]
Species Mus musculus (Mouse)
Source Mammalian cell
Expression Region 24-529aa
Complete Sequence DPASKSRSCSEVRQIYGAKGFSLSDVPQAEISGEHLRICPQGYTCCTSEMEENLANHSRMELESALHDSSRALQATLATQLHGIDDHFQRLLNDSERTLQEAFPGAFGDLYTQNTRAFRDLYAELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLPDDYLDCLGKQAEALRPFGDAPRELRLRATRAFVAARSFVQGLGVASDVVRKVAQVPLAPECSRAIMKLVYCAHCRGVPGARPCPDYCRNVLKGCLANQADLDAEWRNLLDSMVLITDKFWGPSGAESVIGGVHVWLAEAINALQDNKDTLTAKVIQACGNPKVNPHGSGPEEKRRRGKLALQEKPSTGTLEKLVSEAKAQLRDIQDFWISLPGTLCSEKMAMSPASDDRCWNGISKGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRGAYGGNDVDFQDASDDGSGSGSGGGCPDDTCGRRVSKKSSSSRTPLTHALPGLSEQEGQKTS
Mol. Weight 56.7 kDa
Protein Length Full Length of Mature Protein
Tag Info C-terminal 6xHis-tagged
Form Lyophilized powder
Buffer Lyophilized from a 0.2 μm filtered 1xPBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
(From Uniprot)
Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. Binds Cu(2+) or Zn(2+) ions.
Gene References into Functions
  1. This study provides new insights into the roles of GPC1 and GPC3 in mandibular morphogenesis. GPC3-knockout reduces mandibular growth, but GPC1 does not. These findings along with previous studies indicate that glypicans could be potential therapeutic agents to manage craniosynostosis and associated bony dysmorphology. PMID: 28426184
  2. Data show that notum and glypican-1 and glypican-3 gene expression during colorectal cancer (CRC) development and present evidence to suggest them as potential new biomarkers of CRC pathogenesis. PMID: 26517809
  3. Amyloid precursor protein (APP)/APP-like protein 2 (APLP2) expression is required to initiate endosome-nucleus-autophagosome PMID: 24898256
  4. The N-terminal growth-factor-like domain of amyloid-beta precursor protein is necessary for protein-receptor binding, whereas the E2-domain mediates interaction with membrane-anchored syndecan 2 and glypican 1. PMID: 23986479
  5. Glypican-1 is expressed by neural stem cells and neurons derived from embryonic stem cells, which are then surrounded with cell bodies and processes, which in certain cases show distinctive expression patterns. PMID: 20689858
  6. Glypican-1 is a novel cellular cofactor for prion conversion. It acts as a scaffold facilitating the interaction of PrP(C) and PrP(Sc) in lipid rafts. PMID: 19936054
  7. data imply that endogenous Gpc1 is involved in control of growth factor signaling, a finding that is both novel and relevant to the general question of how the activities of co-receptors are exploited during development PMID: 19732411
  8. Data describe a relationship between heparan sulfate and copper binding of amyloid precursor protein (APP) and amyloid precursor-like protein 2 (APLP2) in the modulation of nitroxyl anion-catalyzed heparan sulfate degradation in glypican-1. PMID: 15677459
  9. cancer cell- and host-derived GPC1 are crucial for full mitogenic, angiogenic, and metastatic potential of cancer cells PMID: 18064304
  10. GPC1 regulates EC cell cycle progression at least partially by modulating APC/C-mediated degradation of mitotic cyclins and securin PMID: 18417614
  11. These results suggest a role for Gpc-1 autoprocessing in the clearance of PrP(Sc) from infected cells. PMID: 18717736
  12. GPC1 may have an important role in the establishment of a microenvironment that supports early events in hematopoiesis. PMID: 18840400

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Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side, Endosome
Protein Families Glypican family
Database Links

KEGG: mmu:14733

STRING: 10090.ENSMUSP00000047199

UniGene: Mm.297976

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