Product Details

Purity

>85% (SDS-PAGE)

Target Names

Gpc1

Uniprot No.

Q9QZF2

Alternative Names

Gpc1; Glypican-1 [Cleaved into: Secreted glypican-1]

Species

Mus musculus (Mouse)

Expression Region

24-529

Target Protein Sequence

DPASKSR SCSEVRQIYG AKGFSLSDVP QAEISGEHLR ICPQGYTCCT SEMEENLANH SRMELESALH DSSRALQATL ATQLHGIDDH FQRLLNDSER TLQEAFPGAF GDLYTQNTRA FRDLYAELRL YYRGANLHLE ETLAEFWARL LERLFKQLHP QLLPDDYLDC LGKQAEALRP FGDAPRELRL RATRAFVAAR SFVQGLGVAS DVVRKVAQVP LAPECSRAIM KLVYCAHCRG VPGARPCPDY CRNVLKGCLA NQADLDAEWR NLLDSMVLIT DKFWGPSGAE SVIGGVHVWL AEAINALQDN KDTLTAKVIQ ACGNPKVNPH GSGPEEKRRR GKLALQEKPS TGTLEKLVSE AKAQLRDIQD FWISLPGTLC SEKMAMSPAS DDRCWNGISK GRYLPEVMGD GLANQINNPE VEVDITKPDM TIRQQIMQLK IMTNRLRGAY GGNDVDFQDA SDDGSGSGSG GGCPDDTCGR RVSKKSSSSR TPLTHALPGL SEQEGQKTS

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. Binds Cu(2+) or Zn(2+) ions.

Gene References into Functions

This study provides new insights into the roles of GPC1 and GPC3 in mandibular morphogenesis. GPC3-knockout reduces mandibular growth, but GPC1 does not. These findings along with previous studies indicate that glypicans could be potential therapeutic agents to manage craniosynostosis and associated bony dysmorphology. PMID: 28426184
Data show that notum and glypican-1 and glypican-3 gene expression during colorectal cancer (CRC) development and present evidence to suggest them as potential new biomarkers of CRC pathogenesis. PMID: 26517809
Amyloid precursor protein (APP)/APP-like protein 2 (APLP2) expression is required to initiate endosome-nucleus-autophagosome PMID: 24898256
The N-terminal growth-factor-like domain of amyloid-beta precursor protein is necessary for protein-receptor binding, whereas the E2-domain mediates interaction with membrane-anchored syndecan 2 and glypican 1. PMID: 23986479
Glypican-1 is expressed by neural stem cells and neurons derived from embryonic stem cells, which are then surrounded with cell bodies and processes, which in certain cases show distinctive expression patterns. PMID: 20689858
Glypican-1 is a novel cellular cofactor for prion conversion. It acts as a scaffold facilitating the interaction of PrP(C) and PrP(Sc) in lipid rafts. PMID: 19936054
data imply that endogenous Gpc1 is involved in control of growth factor signaling, a finding that is both novel and relevant to the general question of how the activities of co-receptors are exploited during development PMID: 19732411
Data describe a relationship between heparan sulfate and copper binding of amyloid precursor protein (APP) and amyloid precursor-like protein 2 (APLP2) in the modulation of nitroxyl anion-catalyzed heparan sulfate degradation in glypican-1. PMID: 15677459
cancer cell- and host-derived GPC1 are crucial for full mitogenic, angiogenic, and metastatic potential of cancer cells PMID: 18064304
GPC1 regulates EC cell cycle progression at least partially by modulating APC/C-mediated degradation of mitotic cyclins and securin PMID: 18417614
These results suggest a role for Gpc-1 autoprocessing in the clearance of PrP(Sc) from infected cells. PMID: 18717736
GPC1 may have an important role in the establishment of a microenvironment that supports early events in hematopoiesis. PMID: 18840400

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Endosome. Note=S-nitrosylated form recycled in endosomes. Localizes to CAV1-containing vesicles close to the cell surface. Cleavage of heparan sulfate side chains takes place mainly in late endosomes. Associates with both forms of PRNP in lipid rafts. Colocalizes with APP in perinuclear compartments and with CP in intracellular compartments. Associates with fibrillar APP amyloid-beta peptides in lipid rafts in Alzheimer disease brains.; [Secreted glypican-1]: Secreted, extracellular space.

Protein Families

Glypican family

Database Links

KEGG: mmu:14733

STRING: 10090.ENSMUSP00000047199

UniGene: Mm.297976

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Recombinant Mouse Glypican-1 (Gpc1)

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