2-hydroxyisobutyryl-HIST1H2BC (K20) Antibody

Datasheet
Code CSB-PA010403OA20hibHU
Size US$319
Uniprot No. P62807
Image
  • Western Blot
    Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h
    All lanes: HIST1H2BC antibody at 1:100
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 14 kDa
    Observed band size: 14 kDa

  • Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010403OA20hibHU at 1:7.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunogen Peptide sequence around site of 2-hydroxyisobutyryl-Lys (20) derived from Human Histone H2B
Raised in Rabbit
Species Reactivity Human, Rat
Tested Applications ELISA, WB, IF; Recommended dilution: WB:1:100-1:1000, IF:1:50-1:200
Relevance Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Research Area Epigenetics and Nuclear Signaling
Target Names HIST1H2BC
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; FUNCTION
Subcellular Location Nucleus, Chromosome
Protein Families Histone H2B family
Database Links

HGNC: 4757

OMIM: 602798

KEGG: hsa:3017

STRING: 9606.ENSP00000366962

UniGene: Hs.182137

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