AACS Antibody

Datasheet
Code CSB-PA001012LA01HU
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Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. Q86V21
Image
  • Western Blot
    Positive WB detected in: HEK293 whole cell lysate
    All lanes: AACS antibody at 2.5µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 76, 68, 31 kDa
    Observed band size: 76 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA001012LA01HU at dilution of 1:100

  • Immunoprecipitating AACS in HEK293 whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA001012LA01HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA001012LA01HU (8µg) + HEK293 whole cell lysate (500µg)
    Lane 3: HEK293 whole cell lysate (10µg)

Immunogen Recombinant Human Acetoacetyl-CoA synthetase protein (435-598AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000
Relevance Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development (By similarity).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Acetoacetyl-CoA synthetase (EC 6.2.1.16) (Acyl-CoA synthetase family member 1) (Protein sur-5 homolog), AACS, ACSF1
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Research Area Signal Transduction
Target Names AACS
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development (By similarity).
Subcellular Location Cytoplasm, cytosol
Protein Families ATP-dependent AMP-binding enzyme family
Tissue Specificity Highly expressed in kidney, heart and brain, but low in liver.
Database Links

HGNC: 21298

OMIM: 614364

KEGG: hsa:65985

STRING: 9606.ENSP00000324842

UniGene: Hs.656073

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