AACS Antibody

Code CSB-PA001012LA01HU
Size US$299 How to order?
Image
  • Western Blot
    Positive WB detected in: HEK293 whole cell lysate
    All lanes: AACS antibody at 2.5µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 76, 68, 31 kDa
    Observed band size: 76 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA001012LA01HU at dilution of 1:100

  • Immunoprecipitating AACS in HEK293 whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA001012LA01HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA001012LA01HU (8µg) + HEK293 whole cell lysate (500µg)
    Lane 3: HEK293 whole cell lysate (10µg)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) AACS Polyclonal antibody
Uniprot No. Q86V21
Target Names AACS
Alternative Names AACS antibody; ACSF1Acetoacetyl-CoA synthetase antibody; EC 6.2.1.16 antibody; Acyl-CoA synthetase family member 1 antibody; Protein sur-5 homolog antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Acetoacetyl-CoA synthetase protein (435-598AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated

The AACS Antibody (Product code: CSB-PA001012LA01HU) is Non-conjugated. For AACS Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA001012LB01HU AACS Antibody, HRP conjugated ELISA
FITC CSB-PA001012LC01HU AACS Antibody, FITC conjugated
Biotin CSB-PA001012LD01HU AACS Antibody, Biotin conjugated ELISA
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IP 1:200-1:2000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development.
Gene References into Functions
  1. the human AACS promoter is a PPARgamma target gene and this nuclear receptor is recruited to the AACS promoter by direct interaction with Sp1 PMID: 20102333
Subcellular Location Cytoplasm, cytosol.
Protein Families ATP-dependent AMP-binding enzyme family
Tissue Specificity Highly expressed in kidney, heart and brain, but low in liver.
Database Links

HGNC: 21298

OMIM: 614364

KEGG: hsa:65985

STRING: 9606.ENSP00000324842

UniGene: Hs.656073

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