AACS Antibody

Datasheet
Code CSB-PA001012LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: HEK293 whole cell lysate
    All lanes: AACS antibody at 2.5µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 76, 68, 31 kDa
    Observed band size: 76 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA001012LA01HU at dilution of 1:100

  • Immunoprecipitating AACS in HEK293 whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA001012LA01HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA001012LA01HU (8µg) + HEK293 whole cell lysate (500µg)
    Lane 3: HEK293 whole cell lysate (10µg)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) AACS Polyclonal antibody
Uniprot No. Q86V21
Target Names AACS
Alternative Names AACS antibody; ACSF1Acetoacetyl-CoA synthetase antibody; EC 6.2.1.16 antibody; Acyl-CoA synthetase family member 1 antibody; Protein sur-5 homolog antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Acetoacetyl-CoA synthetase protein (435-598AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IP 1:200-1:2000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development (By similarity).
Gene References into Functions
  1. the human AACS promoter is a PPARgamma target gene and this nuclear receptor is recruited to the AACS promoter by direct interaction with Sp1 PMID: 20102333
Subcellular Location Cytoplasm, cytosol
Protein Families ATP-dependent AMP-binding enzyme family
Tissue Specificity Highly expressed in kidney, heart and brain, but low in liver.
Database Links

HGNC: 21298

OMIM: 614364

KEGG: hsa:65985

STRING: 9606.ENSP00000324842

UniGene: Hs.656073

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