AACS Antibody

Datasheet

Code	CSB-PA001012LA01HU
Size	US$299Purchase it in Cusabio online store (only available for customers from the US)
Image	Western Blot Positive WB detected in: HEK293 whole cell lysate All lanes: AACS antibody at 2.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76, 68, 31 kDa Observed band size: 76 kDa Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA001012LA01HU at dilution of 1:100 Immunoprecipitating AACS in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA001012LA01HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA001012LA01HU (8µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (10µg)
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Product Details
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Target Data

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) AACS Polyclonal antibody

Uniprot No.

Q86V21

Target Names

AACS

Alternative Names

AACS antibody; ACSF1Acetoacetyl-CoA synthetase antibody; EC 6.2.1.16 antibody; Acyl-CoA synthetase family member 1 antibody; Protein sur-5 homolog antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Acetoacetyl-CoA synthetase protein (435-598AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function	Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development (By similarity).
Gene References into Functions	the human AACS promoter is a PPARgamma target gene and this nuclear receptor is recruited to the AACS promoter by direct interaction with Sp1 PMID: 20102333
Subcellular Location	Cytoplasm, cytosol
Protein Families	ATP-dependent AMP-binding enzyme family
Tissue Specificity	Highly expressed in kidney, heart and brain, but low in liver.
Database Links	HGNC: 21298 OMIM: 614364 KEGG: hsa:65985 STRING: 9606.ENSP00000324842 UniGene: Hs.656073