AAK1 Antibody

Datasheet
Code CSB-PA643571LA01HU
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Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. Q2M2I8
Image
  • Western Blot
    Positive WB detected in: Mouse kidney tissue
    All lanes: AAK1 antibody at 2.5μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 104, 94 kDa
    Observed band size: 104 kDa

  • Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA643571LA01HU at dilution of 1:100

  • Immunofluorescent analysis of HepG2 cells using CSB-PA643571LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunogen Recombinant Human AP2-associated protein kinase 1 protein (274-465AA)
Raised in Rabbit
Species Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Relevance Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias AP2-associated protein kinase 1 (EC 2.7.11.1) (Adaptor-associated kinase 1), AAK1, KIAA1048
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Signal Transduction
Target Names AAK1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.
Subcellular Location Cell membrane, Peripheral membrane protein, Membrane, clathrin-coated pit
Protein Families Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.
Database Links

HGNC: 19679

OMIM: 616405

KEGG: hsa:22848

STRING: 9606.ENSP00000386456

UniGene: Hs.468878

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