ACOT1 Antibody

Code CSB-PA768206LA01HU
Size US$166
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Image
  • IHC image of CSB-PA768206LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA768206LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) ACOT1 Polyclonal antibody
Uniprot No.
Target Names
ACOT1
Alternative Names
ACOT1 antibody; CTE1Acyl-coenzyme A thioesterase 1 antibody; Acyl-CoA thioesterase 1 antibody; EC 3.1.2.- antibody; CTE-I antibody; CTE-Ib antibody; Inducible cytosolic acyl-coenzyme A thioester hydrolase antibody; Long chain acyl-CoA thioester hydrolase antibody; Long chain acyl-CoA hydrolase antibody; Palmitoyl-coenzyme A thioesterase antibody; EC 3.1.2.2 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Acyl-coenzyme A thioesterase 1 protein (258-350AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The ACOT1 Antibody (Product code: CSB-PA768206LA01HU) is Non-conjugated. For ACOT1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA768206LB01HU ACOT1 Antibody, HRP conjugated ELISA
FITC CSB-PA768206LC01HU ACOT1 Antibody, FITC conjugated
Biotin CSB-PA768206LD01HU ACOT1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:200-1:500
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels. More active towards saturated and unsaturated long chain fatty acyl-CoAs (C12-C20).
Subcellular Location
Cytoplasm, cytosol.
Protein Families
C/M/P thioester hydrolase family
Database Links

HGNC: 33128

OMIM: 614313

KEGG: hsa:641371

STRING: 9606.ENSP00000311224

UniGene: Hs.446685

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