ADGRA2 Antibody

Datasheet

Code	CSB-PA857001LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: A549 whole cell lysate, Rat heart tissue All lanes: ADGRA2 antibody at 3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 143, 120, 20 kDa Observed band size: 143, 120 kDa Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA857001LA01HU at dilution of 1:100 Immunofluorescent analysis of HepG2 cells using CSB-PA857001LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ADGRA2 Polyclonal antibody

Uniprot No.

Q96PE1

Target Names

ADGRA2

Alternative Names

DKFZp434C211 antibody; DKFZp434J0911 antibody; FLJ14390 antibody; G protein coupled receptor 124 antibody; G-protein coupled receptor 124 antibody; GP124_HUMAN antibody; GPR 124 antibody; Gpr124 antibody; KIAA1531 antibody; TEM 5 antibody; TEM5 antibody; Tumor endothelial marker 5 antibody; Tumor endothelial marker 5 precursor antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Adhesion G protein-coupled receptor A2 protein (1069-1338AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ADGRA2 Antibody (Product code: CSB-PA857001LA01HU) is Non-conjugated. For ADGRA2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA857001LB01HU	ADGRA2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA857001LC01HU	ADGRA2 Antibody, FITC conjugated
Biotin	CSB-PA857001LD01HU	ADGRA2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Endothelial receptor which functions together with RECK to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B). Plays a key role in Wnt7-specific responses, such as endothelial cell sprouting and migration in the forebrain and neural tube, and establishment of the blood-brain barrier. Acts as a Wnt7-specific coactivator of canonical Wnt signaling: required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex. ADGRA2-tethering function does not rely on its G-protein coupled receptor (GPCR) structure but instead on its combined capacity to interact with RECK extracellularly and recruit the Dishevelled scaffolding protein intracellularly. Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate.

Gene References into Functions

Data suggest that GPR124 promotes cell adhesion via interaction with Elmo1-Dock180 and intersectin 1/2; this constitutes a previously unrecognized heteromeric complex that is putatively involved in GPR124-dependent adhesive/angiogenic responses in vascular endothelial cells. (GPR124 = G-protein coupled receptor 124; Elmo1 = ELMO domain-containing protein 1; Dock180 = dedicator of cytokinesis protein 1 180 kDa) PMID: 28600358
Observed an inverse correlation between the expression of miR-138-5p and GPR124 in lung adenocarcinoma specimens. Knockdown of GPR124 mimicked the effects of miR-138-5p on the sensitivity to gefitinib. PMID: 24582749
Thrombin-induced shedding of tumour endothelial marker 5 and exposure of its RGD motif are regulated by cell-surface protein disulfide-isomerase. PMID: 22013897
TEM5 expression during capillary morphogenesis is induced by the small GTPase Rac and mediates contact inhibition of proliferation in endothelial cells. PMID: 19853600
Proteolytically processed soluble tumor endothelial marker TEM5 mediates endothelial cell survival during angiogenesis by linking integrin alpha(v)beta3 to glycosaminoglycans PMID: 16982628

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Cell projection, filopodium.

Protein Families

G-protein coupled receptor 2 family, Adhesion G-protein coupled receptor (ADGR) subfamily

Tissue Specificity

Expressed in endothelial cells (at protein level). Abundantly expressed in heart, placenta, ovary, small intestine, and colon.

Database Links

HGNC: 17849

OMIM: 606823

KEGG: hsa:25960

STRING: 9606.ENSP00000406367

UniGene: Hs.274136

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

G protein coupled receptor signaling pathway Target Therapy

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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