ADH5 Antibody

Code CSB-PA001357LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
  • Western Blot
    Positive WB detected in: Rat liver tissue
    All lanes: ADH5 antibody at 2μg/ml
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA001357LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA001357LA01HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA001357LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

The Latest Promotion Free Antibody trial simple
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) ADH5 Polyclonal antibody
Uniprot No. P11766
Target Names ADH5
Alternative Names ADH 3 antibody; ADH5 antibody; ADHX antibody; ADHX_HUMAN antibody; Alcohol dehydrogenase (class III) chi polypeptide antibody; alcohol dehydrogenase 5 (class III) chi polypeptide antibody; Alcohol dehydrogenase 5 antibody; Alcohol dehydrogenase class 3 antibody; Alcohol dehydrogenase class chi chain antibody; Alcohol dehydrogenase class III antibody; Alcohol dehydrogenase class-3 antibody; Alcohol dehydrogenase class-III antibody; class III alcohol dehydrogenase 5 chi subunit antibody; FALDH antibody; FDH antibody; formaldehyde dehydrogenase antibody; Glutathione dependent formaldehyde dehydrogenase antibody; Glutathione-dependent formaldehyde dehydrogenase antibody; GSH-FDH antibody; GSNOR antibody; hydroxymethyllutathione dehydrogenase antibody; S-(hydroxymethyl)glutathione dehydrogenase antibody
Raised in Rabbit
Species Reactivity Human, Rat
Immunogen Recombinant Human Alcohol dehydrogenase class-3 protein (14-130AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.
Gene References into Functions
  1. Data (including data from studies using knockout and transgenic mice) suggest that obesity and diabetes are accompanied by decreases in GSNOR activity in hepatocytes engendering nitrosative stress; obesity promotes S-nitrosylation of lysosomal proteins in liver, thereby impairing lysosomal enzyme activities and compromising autophagy. PMID: 29074597
  2. GSNOR represents the prototype enzyme to disclose how denitrosylation plays a crucial role in tuning NO-bioactivity and how much it deeply impacts on cell homeostasis and human pathophysiology. (Review) PMID: 28533171
  3. It was concluded that in HepG2 cells, ADH5 is a source of formate for de novo purine biosynthesis, especially during folate deficiency when folate-dependent formate production is limited. PMID: 28228507
  4. GSNOR expression has different effect on neuronal viability in dependence on the stimulus applied, and plays opposite roles in SH-SY5Y models of Parkinson's disease and amyotrophic lateral sclerosis PMID: 26491229
  5. ADH5 counteracts neuronal differentiation of neural stem cells and this effect can be reversed by pharmacological inhibition of ADH5. PMID: 24895131
  6. study compared individuals occupationally exposed to formaldehyde and controls to effects of XRCC3 Thr241Met, ADH5 Val309Ile and Asp353Glu polymorphisms; ADH5 polymorphisms did not show significant association with genotoxicity biomarkers PMID: 23355119
  7. N6022 binds in the GSNO binding pocket like a competitive inhibitor, although in kinetic assays it behaves with a mixed uncompetitive mode of inhibition (MOI) toward GSNO and a mixed competitive MOI toward formaldehyde adduct S-hydroxymethylglutathione. PMID: 22335564
  8. biological significance of SNPs rs11568816, rs17028487 and rs13832 PMID: 21920416
  9. Data suggest that GSNOR deficiency, through dysregulated S-nitrosylation, may promote hepatocellular carcinoma, possibly by inactivating a DNA repair system. PMID: 20371487
  10. Significant associations were found however, for reactions to alcohol with a SNP in ADH5 (rs6827292, p = .001) and a SNP just upstream of ADH5 (rs6819724, p = .0007) that is in strong LD with rs6827292. PMID: 20158305
  11. POZ domain of FBI1 represses transcription of ADH5. PMID: 12004059
  12. The structural determination of apo, binary alcohol, and inhibitory ternary FDH complexes provides new insight into the enzyme's metal-assisted catalysis and substrate-binding properties. PMID: 12196016
  13. Formation of a FDH-S-(hydroxymethyl)glutathione-NADH ternary complex causes movement of glutathione-dependent formaldehyde dehydrogenase catalytic domain toward the coenzyme-binding domain, and a change in active-site zinc coordination. PMID: 12484756
  14. No evidence that alcohol dehydrogenase 3genotype modifies risk related to alcohol and lung cancer. PMID: 14608084
  15. data suggest that genetic variation in S-nitrosoglutathione reductase (GSNOR) might play a role in asthma susceptibility PMID: 17543375
  16. A statistically significant increase of class III alcohol dehydrogenase isoenzymes was found in the sera of pancreatic cancer patients. PMID: 18095160
  17. GSNOR inhibitors to be novel tools for regulating nitric oxide bioactivity and assessing the role of SNOs in vivo. PMID: 19596685

Show More

Hide All

Subcellular Location Cytoplasm
Protein Families Zinc-containing alcohol dehydrogenase family, Class-III subfamily
Database Links

HGNC: 253

OMIM: 103710

KEGG: hsa:128

STRING: 9606.ENSP00000296412

UniGene: Hs.78989


Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1