AKR1B1 Antibody

Code CSB-PA001539LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
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  • Western Blot
    Positive WB detected in: Hela whole cell lysate
    All lanes: AKR1B1 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 36 kDa
    Observed band size: 36, 50 kDa

  • Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA001539LA01HU at dilution of 1:100

  • Immunofluorescent analysis of A549 cells using CSB-PA001539LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) AKR1B1 Polyclonal antibody
Uniprot No. P15121
Target Names AKR1B1
Alternative Names ADR antibody; AKR1B 1 antibody; Akr1b1 antibody; Aldehyde reductase 1 antibody; Aldehyde reductase antibody; Aldo keto reductase family 1; member B1 antibody; Aldo-keto reductase family 1 member B1 antibody; aldo-keto reductase family 1; member B1 (aldose reductase) antibody; Aldose reductase antibody; aldr 1 antibody; ALDR_HUMAN antibody; aldr1 antibody; ALR2 antibody; AR antibody; Lii5 2 CTCL tumor antigen antibody; Low Km aldose reductase antibody; MGC1804 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Aldose reductase protein (114-230AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Association Analysis of Single-Cell RNA Sequencing and Proteomics Reveals a Vital Role of Ca2+ Signaling in the Determination of Skeletal Muscle Development Potential.. K Qiu,Cells,2020

Applications: Western Blot
Sample type: Muscle Myo-lineage cells
Review: In order to verify the reliability of proteomics data, 7 DEPs were randomly selected for Western blot analysis. As shownin FigureS1, there lativea bundance sof selected proteins between Myo-L and Myo-Y determined by Western blot were highly consistent with the data of TMT analysis.
PMID: 32331484

Target Data

Function Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
Gene References into Functions
  1. Here, we show that treatment of colorectal cancer (CRC) cells with fidarestat increases the efficacy of doxorubicin (DOX)-induced death in HT-29 and SW480 cells and in nude mice xenografts. Aldose reductase inhibition also results in higher intracellular accumulation of DOX and decreases the expression of drug transporter proteins MDR1, MRP1, and ABCG2. PMID: 28600556
  2. AKR1B1 rs759853 polymorphism had no association with diabetic retinopathy (DR) risk under all genetic models. However, after subgroup analysis by diabetes mellitus; type, the rs759853 polymorphism was a protective factor against the DR onset in patients with type 1 diabetes mellitus; Subgroup analysis by genotyping method suggested that rs759853 was significantly correlated with decreased risk of DR under dominate model PMID: 30201105
  3. A combined gene expression signature of AKR1B10 (low) and AKR1B1 (high) showed a better prognostic stratification of CRC patients independent of confounding factors. PMID: 28929377
  4. Data show that cells with higher levels of aldo-keto reductases AKR1B1 and/or AKR1B10 (AKR1Bs) were more sensitive to 2-deoxyglucose (2DG). PMID: 29617059
  5. genetic association studies in population in north India: Data suggest that an SNP in promoter region of aldose reductase (C-106T) is associated with peripheral neuropathy in patients with type 2 diabetes mellitus in the population studied. PMID: 28495421
  6. Under hyperglycemic conditions, aldose reductase (AR)-mediated sorbitol formation and associated rise in cell volume, which subsequently results in platelet hyperactivation, occur. PMID: 28820747
  7. In the Eastern Asians with type 2 diabetes mellitus, the AR gene C-106T gene polymorphism is correlated with an increased risk of diabetic nephropathy; the Eastern Asians with the T allele of AR gene C-106T gene polymorphism might be susceptible to DN PMID: 28651212
  8. An meta-analysis showed that aldose reductase C-106T variants appear to influence the risk for diabetic retinopathy in Chinese Han persons (meta-analysis). PMID: 26580232
  9. AKR1B1 as a key modulator of tumor aggressiveness and suggests that pharmacologic inhibition of AKR1B1 has the potential to become a valuable therapeutic strategy for Basal-like breast cancer (BLBC). PMID: 28270406
  10. inhibiting AR or degrading H2O2 could protect endothelial function and maintain the antioxidant activities of uric acid. PMID: 28057038
  11. Result indicate that the differential scanning fluorimetry (DSF) method is useful for enzyme inhibitor drug screening for the AKR superfamily, including AKR1B10 and a structurally similar isoform AKR1B1. PMID: 28003428
  12. The hyperosmotic AR gene expression was dependent on activation of metalloproteinases, autocrine/paracrine TGF-beta signaling, activation of p38 MAPK, ERK1/2, and PI3K signal transduction pathways, and the transcriptional activity of NFAT5. PMID: 27628063
  13. Aberrant DNA methylation of AKR1B1 could be potential screening markers of colorectal cancer. PMID: 27493446
  14. -106T allele of AKR1B1 C-106T polymorphism may be associated with increased risk for essential hypertension in Chinese Han population. PMID: 27343777
  15. These findings suggest a statistically significant association of AKR1B1 -106C>T polymorphism with retinopathy in North Indian patients PMID: 27640118
  16. mRNA expression in macrophages correlates positively with M1 polarization, and depends on hyperglycemia PMID: 26873505
  17. Meta-analysis shows that the AR rs759853 polymorphism may correlate with the susceptibility of DN. However, data do not support the association between this DNA variation and the progression of DN. PMID: 25885804
  18. ALR C(-106)T polymorphism was not associated with an increased risk of Diabetic Retinopathy; subgroup analysis showed a genetic association between ALR C(-106)T polymorphism and the risk of Diabetic Retinopathy of type 1 Diabetes but not Diabetic Retinopathy of type 2 Diabetes(Meta-Analysis) PMID: 25722213
  19. Higher expression of PLA2G2A, PTGS2, AKR1B1, AKR1C3 and ABCC4 was seen in 22-B endometriosis cells. PMID: 25446850
  20. Data conclude that AKR1B1 and TM6SF1 may serve as candidate methylation biomarkers for early breast cancer detection. PMID: 25123395
  21. L-idose is the best alternative to D-glucose in studies on aldose reductase. PMID: 25528584
  22. role of the human aldose reductase AKR1B1 in prostaglandin (PG) F2 alpha synthesis in human subcutaneous and omental adipose tissue PMID: 24663124
  23. One of the most striking changes involved sorbitol dehydrogenase, a key enzyme in the polyol pathway. Validation studies revealed dramatically increased sorbitol dehydrogenase concentrations and activity in adenomas and cancer cell lines, along with important changes in the expression of other enzymes in the same (AKR1B1) and related (KHK) pathways. PMID: 24567419
  24. In type 2 diabetic patients with suboptimal glycaemic control, the z-4 allele of ALR2 (CA)n polymorphism was independently associated with increased susceptibility to cataracts. PMID: 24360973
  25. prostaglandin F synthase activity of human and bovine aldo-keto reductases PMID: 23747692
  26. Aldose reductase contributes to diabetes-mediated mitochondrial dysfunction and damage through the activation of p53. PMID: 24474649
  27. Aldose reductase gene may not be significantly associated with diabetic retinopathy in Chinese patients with type 2 diabetes mellitus. PMID: 24698671
  28. These studies demonstrated sustained activation of Egr-1 with subsequent induction of its downstream target genes in diabetic mouse aortas and in high glucose-treated primary murine aortic endothelial cells expressing human aldose reductase. PMID: 24186862
  29. molecular dynamics simulations were carried out to compute the electric field shift in human aldose reductase PMID: 23517423
  30. A hydrogen bond stabilized active site tryptophan conformation restricts inhibitor access in AKR1B1 compared with the more open AKR1B10 active site. PMID: 24100137
  31. AR inhibition regulates AKT/PI3K-dependent activation of forkhead transcription factor FOXO3a PMID: 23732517
  32. these results show a novel role of AR in mediation of growth factor-induced colon Aldose reductase inhibition prevents colon cancer growth by restoring phosphatase and tensin homolog through modulation of miR-21 and FOXO3a. PMID: 22978663
  33. There were significantly lower mRNA and protein levels of AKR1B1 in cancerous tissues. PMID: 23146748
  34. aldose reductase C-106T genetic polymorphism is not associated with essential hypertension PMID: 22561432
  35. Overexpression of aldose reductase in cardiomyocytes leads to cardiac dysfunction with aging and in the setting of reduced fatty acid and increased glucose metabolism. PMID: 23029549
  36. Data suggest that aldose reductase (AR) plays a mediatory role in ocular neovascularization as seen in diabetic retinopathy; inhibition of AR may have therapeutic potential in diabetic retinopathy. PMID: 22658411
  37. analysis of the inhibition of aldose reductase by Gentiana lutea extracts PMID: 22844269
  38. Site-directed mutagenesis of catalytic tetrad of AKR1B1, composed of Tyr, Lys, His and Asp, revealed triad of Asp43, Lys77 and His110, but not Tyr48, acts as a proton donor in most AKR activities, and is crucial for PGD(2) and PGF(2alpha) synthase activities PMID: 21306562
  39. Molecular dynamics simulation has shown the versatile nature of water molecules in bridge H bonding during interaction. Occupancy and life time of water molecules depend on the type of cocrystallized ligand present in the structure. PMID: 22649481
  40. AR has a role in regulating iNOS expression induced by TNF-alpha in cultured human mesangial cells, indicating the novel function of AR in glomerulonephritis. PMID: 21637955
  41. The commonly reported association of AKR1B1 with diabetic retinopathy may be due to an association of the gene with younger age at onset of diabetes. PMID: 20424224
  42. Aldose reductase C-106T gene polymorphism is associated with diabetic retinopathy in Japanese patients with type 2 diabetes. PMID: 21420193
  43. Inhibition of AR prevented infiltration of blood cells, invasion, migration and formation of capillary like structures, and expression of blood vessels markers. PMID: 21409599
  44. ALR2 over-expression is associated with an alteration in the balance between proliferation and apoptosis of epithelial cells in the mouse lens PMID: 21329682
  45. the enzyme activity of AKR1B10 and AKR1B1 toward alpha, beta-unsaturated carbonyl compounds with cellular and dietary origins PMID: 21329684
  46. findings suggest that AR plays an important role in the cellular response to oxidative stress by sequestering ROS and reactive aldehydes generated in keratinocytes PMID: 21182935
  47. study shows that ALR C-106T polymorphism is not associated with carotid atherosclerosis in Chinese patients with type 2 diabetes. PMID: 21294693
  48. activated hAR arises from oxidative modification of Cys-298, a residue near the nicotinamide binding pocket. PMID: 21084309
  49. The human aldose reductase AKR1B1 is a highly functional PGF synthase responsible for PGF2alpha production in the human endometrium and a potential target for treatment of menstrual disorders. PMID: 20943776
  50. AR is a potent regulator of TGF-beta1 induced expression of FN in human mesangial cells. PMID: 19847669
  51. AR could mediate the TGF-beta1-induced FN production, which may associate with AP-1 activation. PMID: 19760097
  52. Exposure to high glucose and overexpression AR increase the expression of fibronectin. PMID: 19821053
  53. AKR1B3 acts as the PGFS in adipocytes and AKR1B3-produced PGF(2alpha) suppresses adipocyte differentiation by acting through FP receptors PMID: 20093363
  54. AR and SOD2 are renal antigens of human membranous nephropathy and oxidative stress may drive glomerular SOD2 expression. PMID: 20150532
  55. Data show that curcumin inhibits ALR2 with an IC(50) of 10 microM in a non-competitive manner. PMID: 19850041
  56. X-ray structure of human aldose reductase holoenzyme in complex with statil determined at a resolution of 2.1 A PMID: 12486717
  57. Polymorphisms in aldose reductase is associated with those diabetic retinopathy patients who had proliferative retinopathy and maculopathy PMID: 12660865
  58. AKR1B1 and CTSH may be good markers for prediction of sensitivity to certain drugs PMID: 14662023
  59. polymorphic in diabetic nephropathy and retinopathy in type 2 diabetes PMID: 14694017
  60. polymorphic in diabetic nephropathy and in retinopathy in type 2 diabetes PMID: 14694018
  61. crystal structure analysis and molecular dynamics simulations PMID: 15162486
  62. expression of cAMP-regulated AKR1B1 is decreased in adrenocortical cancer. PMID: 15181092
  63. AR is a critical regulator of TNF-alpha-induced apoptotic signaling in endothelial cells PMID: 15251463
  64. The C-106T polymorphism of the aldose reductase gene may contribute to an early development of neurophysiologic deterioration in type 2 diabetic patients. PMID: 15277434
  65. Transgenic mice broadly overexpressing human aldose reductase show that AR plays a key role in ischemic injury and impairment of functional and metabolic recovery after ischemia. PMID: 15284219
  66. AR is an obligatory mediator of TNF-alpha signaling leading to an increase in the expression of adhesion molecules and increased binding of monocytes to the endothelium. PMID: 15284221
  67. C-106T polymorphism in the AR gene is a risk factor for development of diabetic nephropathy in type 2 diabetes in patients with poor glycaemic control PMID: 15637423
  68. AKR1B1 polymorphisms were strongly associated with the rate of functional decline of diabetic complications. PMID: 16936152
  69. AR is an obligatory mediator of growth factor-induced up-regulation of COX-2, PGE2, and growth of Caco-2 colon cancer cells. PMID: 17018629
  70. Two X-ray data sets for a complex of human aldose reductase (h-AR) with the inhibitor IDD 594 and the cofactor NADP(+) were collected from two different parts of the same crystal to a resolution of 0.81 A at 15 and 60 K using cold helium gas as cryogen. PMID: 17139089
  71. Expression of AKR1B1 was significantly decreased in PD cases. PMID: 17270157
  72. A novel binding site conformation has been identified in a region of ALR2 where previous complex structures suggested only low adaptability of the binding pocket. PMID: 17418233
  73. Aldose reductase acceleration may affect the peritoneum in nondiabetic patients undergoing peritoneal dialysis via carbonyl and oxidative stress. PMID: 17851230
  74. Aldose reductase gene was identified in the genome-wide loss-of-function genetic screen as putative tumor suppressor located at 7q35. PMID: 17968325
  75. Levels of ALR2 activity as well as sorbitol in erythrocytes may have value as a quantitative trait to be included among other markers to establish a risk profile for development of diabetic retinopathy. PMID: 18385795
  76. Meta-analysis study shows the correlation between the (AC)n dinucleotide repeat polymorphism at the 5' end and the occurrence of diabetic nephropathy in type 1 diabetic subjects in contrast to type 2 diabetic subjects, in which there was no association. PMID: 18434430
  77. oxLDL-induced upregulation of aldose reductase in human macrophages is proinflammatory in foam cells and may represent a potential link among hyperlipidemia, atherosclerosis, and diabetes mellitus. PMID: 18451330
  78. the binding site residues deviating between ALR1 and ALR2 influence ligand affinity in a complex interplay, presumably involving changes of dynamic properties and differences of the solvation/desolvation balance upon ligand binding PMID: 18495158
  79. Genetic polymorphisms of ALR2 independently predicted new onset of renal and cardiorenal end points, with the latter being largely mediated through renal disease in Chinese type 2 diabetic patients. PMID: 18716049
  80. By western blot analysis, AKR1B1 is present in human liver and brain tissue obtained at autopsy. PMID: 19273550
  81. Triiodothyronine regulates AKR1B1 gene expression via a thyroxine receptor response element-dependant mechanism and associates liver cancer. PMID: 19422879

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Subcellular Location Cytoplasm
Protein Families Aldo/keto reductase family
Tissue Specificity Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.
Database Links

HGNC: 381

OMIM: 103880

KEGG: hsa:231

STRING: 9606.ENSP00000285930

UniGene: Hs.521212

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