AMN Antibody

Datasheet

Code	CSB-PA880128LA01HU
Size	US$166
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Image	IHC image of CSB-PA880128LA01HU diluted at 1:600 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA880128LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) AMN Polyclonal antibody

Uniprot No.

Q9BXJ7

Target Names

AMN

Alternative Names

5033428N14Rik antibody; amn antibody; Amnion associated transmembrane protein antibody; Amnionless antibody; Amnionless homolog antibody; AMNLS_HUMAN antibody; AV002116 antibody; PRO1028 antibody; Protein amnionless antibody; Type I transmembrane protein antibody; UNQ513/PRO1028 antibody; Visceral endoderm specific type 1 transmembrane protein antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Protein amnionless protein (25-163AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The AMN Antibody (Product code: CSB-PA880128LA01HU) is Non-conjugated. For AMN Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA880128LB01HU	AMN Antibody, HRP conjugated	ELISA
FITC	CSB-PA880128LC01HU	AMN Antibody, FITC conjugated
Biotin	CSB-PA880128LD01HU	AMN Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Membrane-bound component of the endocytic receptor formed by AMN and CUBN. Required for normal CUBN glycosylation and trafficking to the cell surface. The complex formed by AMN and CUBN is required for efficient absorption of vitamin B12. Required for normal CUBN-mediated protein transport in the kidney (Probable).

Gene References into Functions

Study shows that cubilin mutations (novel and some previously reported) and all previously reported amnionless missense mutations resulted in endoplasmic reticulum retention and completely inhibited amnionless-dependent plasma membrane expression of cubilin. PMID: 29402915
heterozygous mutations in AMN in a family from the United Kingdom with clinical features of Imerslund-Grasbeck Syndrome PMID: 26040326
Our genetic screening of 154 families of patients with inherited cobalamin malabsorption revealed population-specific mutations, mutational hotspots, and functionally distinct regions in the three causal genes: CUBN, AMN, and GIF. PMID: 22929189
We present evidence that this founder mutation causes over 50% of the Imerslund-Grasbeck syndrome (IGS) cases among Arabic, Turkish, and Sephardic Jewish families, and the mutation is as old as human civvlization. PMID: 22078000
amnionless is essential for the correct luminal expression of cubilin in humans. PMID: 21750092
homozygous mutations affecting exons 1-4 of human AMN lead to megaloblastic anemia 1 PMID: 12590260
cubilin and amnionless are subunits of a novel cubilin/amnionless (cubam) complex PMID: 14576052
Recurrent spontaneous abortions may be caused by mutations in the Amnionless gene. PMID: 16403802
This review summarizes recent data on the biological function of amnionless and focuses on its implication in embryonic nutrition and central nervous system malformations. PMID: 17979745

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Involvement in disease

Recessive hereditary megaloblastic anemia 1 (RH-MGA1)

Subcellular Location

[Isoform 1]: Apical cell membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Endosome membrane. Membrane, coated pit.; [Soluble protein amnionless]: Secreted.

Tissue Specificity

Detected in proximal tubules in the kidney cortex (at protein level). Long isoforms are highly expressed in small intestine, colon and kidney (renal proximal tubule epithelial cells). Shorter isoforms are detected at lower levels in testis, thymus and per

Database Links

HGNC: 14604

OMIM: 261100

KEGG: hsa:81693

STRING: 9606.ENSP00000299155

UniGene: Hs.534494

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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