AMN Antibody

Code CSB-PA880128LA01HU
Size US$166
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Image
  • IHC image of CSB-PA880128LA01HU diluted at 1:600 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA880128LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) AMN Polyclonal antibody
Uniprot No.
Target Names
AMN
Alternative Names
5033428N14Rik antibody; amn antibody; Amnion associated transmembrane protein antibody; Amnionless antibody; Amnionless homolog antibody; AMNLS_HUMAN antibody; AV002116 antibody; PRO1028 antibody; Protein amnionless antibody; Type I transmembrane protein antibody; UNQ513/PRO1028 antibody; Visceral endoderm specific type 1 transmembrane protein antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Protein amnionless protein (25-163AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The AMN Antibody (Product code: CSB-PA880128LA01HU) is Non-conjugated. For AMN Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA880128LB01HU AMN Antibody, HRP conjugated ELISA
FITC CSB-PA880128LC01HU AMN Antibody, FITC conjugated
Biotin CSB-PA880128LD01HU AMN Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:500-1:1000
IF 1:200-1:500
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Membrane-bound component of the endocytic receptor formed by AMN and CUBN. Required for normal CUBN glycosylation and trafficking to the cell surface. The complex formed by AMN and CUBN is required for efficient absorption of vitamin B12. Required for normal CUBN-mediated protein transport in the kidney (Probable).
Gene References into Functions
  1. Study shows that cubilin mutations (novel and some previously reported) and all previously reported amnionless missense mutations resulted in endoplasmic reticulum retention and completely inhibited amnionless-dependent plasma membrane expression of cubilin. PMID: 29402915
  2. heterozygous mutations in AMN in a family from the United Kingdom with clinical features of Imerslund-Grasbeck Syndrome PMID: 26040326
  3. Our genetic screening of 154 families of patients with inherited cobalamin malabsorption revealed population-specific mutations, mutational hotspots, and functionally distinct regions in the three causal genes: CUBN, AMN, and GIF. PMID: 22929189
  4. We present evidence that this founder mutation causes over 50% of the Imerslund-Grasbeck syndrome (IGS) cases among Arabic, Turkish, and Sephardic Jewish families, and the mutation is as old as human civvlization. PMID: 22078000
  5. amnionless is essential for the correct luminal expression of cubilin in humans. PMID: 21750092
  6. homozygous mutations affecting exons 1-4 of human AMN lead to megaloblastic anemia 1 PMID: 12590260
  7. cubilin and amnionless are subunits of a novel cubilin/amnionless (cubam) complex PMID: 14576052
  8. Recurrent spontaneous abortions may be caused by mutations in the Amnionless gene. PMID: 16403802
  9. This review summarizes recent data on the biological function of amnionless and focuses on its implication in embryonic nutrition and central nervous system malformations. PMID: 17979745

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Involvement in disease
Recessive hereditary megaloblastic anemia 1 (RH-MGA1)
Subcellular Location
[Isoform 1]: Apical cell membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Endosome membrane. Membrane, coated pit.; [Soluble protein amnionless]: Secreted.
Tissue Specificity
Detected in proximal tubules in the kidney cortex (at protein level). Long isoforms are highly expressed in small intestine, colon and kidney (renal proximal tubule epithelial cells). Shorter isoforms are detected at lower levels in testis, thymus and per
Database Links

HGNC: 14604

OMIM: 261100

KEGG: hsa:81693

STRING: 9606.ENSP00000299155

UniGene: Hs.534494

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